GNPI_AEDAE
ID GNPI_AEDAE Reviewed; 278 AA.
AC Q16HW7; Q16HW8; Q16HW9;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 25-JUL-2006, sequence version 1.
DT 25-MAY-2022, entry version 95.
DE RecName: Full=Glucosamine-6-phosphate isomerase;
DE EC=3.5.99.6;
DE AltName: Full=Glucosamine-6-phosphate deaminase;
DE Short=GNPDA;
DE Short=GlcN6P deaminase;
GN Name=Gnpda1; ORFNames=AAEL013877;
OS Aedes aegypti (Yellowfever mosquito) (Culex aegypti).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Nematocera; Culicoidea; Culicidae;
OC Culicinae; Aedini; Aedes; Stegomyia.
OX NCBI_TaxID=7159;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND ALTERNATIVE SPLICING
RP (ISOFORMS A; B AND E).
RC STRAIN=LVPib12;
RX PubMed=17510324; DOI=10.1126/science.1138878;
RA Nene V., Wortman J.R., Lawson D., Haas B.J., Kodira C.D., Tu Z.J.,
RA Loftus B.J., Xi Z., Megy K., Grabherr M., Ren Q., Zdobnov E.M., Lobo N.F.,
RA Campbell K.S., Brown S.E., Bonaldo M.F., Zhu J., Sinkins S.P.,
RA Hogenkamp D.G., Amedeo P., Arensburger P., Atkinson P.W., Bidwell S.L.,
RA Biedler J., Birney E., Bruggner R.V., Costas J., Coy M.R., Crabtree J.,
RA Crawford M., DeBruyn B., DeCaprio D., Eiglmeier K., Eisenstadt E.,
RA El-Dorry H., Gelbart W.M., Gomes S.L., Hammond M., Hannick L.I.,
RA Hogan J.R., Holmes M.H., Jaffe D., Johnston S.J., Kennedy R.C., Koo H.,
RA Kravitz S., Kriventseva E.V., Kulp D., Labutti K., Lee E., Li S.,
RA Lovin D.D., Mao C., Mauceli E., Menck C.F., Miller J.R., Montgomery P.,
RA Mori A., Nascimento A.L., Naveira H.F., Nusbaum C., O'Leary S.B., Orvis J.,
RA Pertea M., Quesneville H., Reidenbach K.R., Rogers Y.-H.C., Roth C.W.,
RA Schneider J.R., Schatz M., Shumway M., Stanke M., Stinson E.O.,
RA Tubio J.M.C., Vanzee J.P., Verjovski-Almeida S., Werner D., White O.R.,
RA Wyder S., Zeng Q., Zhao Q., Zhao Y., Hill C.A., Raikhel A.S., Soares M.B.,
RA Knudson D.L., Lee N.H., Galagan J., Salzberg S.L., Paulsen I.T.,
RA Dimopoulos G., Collins F.H., Bruce B., Fraser-Liggett C.M., Severson D.W.;
RT "Genome sequence of Aedes aegypti, a major arbovirus vector.";
RL Science 316:1718-1723(2007).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=alpha-D-glucosamine 6-phosphate + H2O = beta-D-fructose 6-
CC phosphate + NH4(+); Xref=Rhea:RHEA:12172, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:28938, ChEBI:CHEBI:57634, ChEBI:CHEBI:75989; EC=3.5.99.6;
CC -!- SUBUNIT: Homohexamer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=E; Synonyms=F, G;
CC IsoId=Q16HW7-1; Sequence=Displayed;
CC Name=B; Synonyms=C, D;
CC IsoId=Q16HW7-2; Sequence=VSP_032747;
CC Name=A;
CC IsoId=Q16HW7-3; Sequence=VSP_032746;
CC -!- SIMILARITY: Belongs to the glucosamine/galactosamine-6-phosphate
CC isomerase family. {ECO:0000305}.
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DR EMBL; CH478133; EAT33845.1; -; Genomic_DNA.
DR EMBL; CH478133; EAT33846.1; -; Genomic_DNA.
DR EMBL; CH478133; EAT33847.1; -; Genomic_DNA.
DR EMBL; CH478133; EAT33848.1; -; Genomic_DNA.
DR EMBL; CH478133; EAT33849.1; -; Genomic_DNA.
DR EMBL; CH478133; EAT33850.1; -; Genomic_DNA.
DR EMBL; CH478133; EAT33851.1; -; Genomic_DNA.
DR RefSeq; XP_001657190.1; XM_001657140.1.
DR RefSeq; XP_001657191.1; XM_001657141.1.
DR RefSeq; XP_001657192.1; XM_001657142.1.
DR RefSeq; XP_001657193.1; XM_001657143.1.
DR RefSeq; XP_001657194.1; XM_001657144.1.
DR RefSeq; XP_001657195.1; XM_001657145.1.
DR RefSeq; XP_001657196.1; XM_001657146.1.
DR AlphaFoldDB; Q16HW7; -.
DR SMR; Q16HW7; -.
DR STRING; 7159.AAEL013877-PG; -.
DR VEuPathDB; VectorBase:AAEL013877; -.
DR eggNOG; KOG3148; Eukaryota.
DR InParanoid; Q16HW7; -.
DR OMA; FNEPCSS; -.
DR OrthoDB; 1425290at2759; -.
DR PhylomeDB; Q16HW7; -.
DR Proteomes; UP000008820; Chromosome 2.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0004342; F:glucosamine-6-phosphate deaminase activity; ISS:UniProtKB.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0006091; P:generation of precursor metabolites and energy; ISS:UniProtKB.
DR GO; GO:0006043; P:glucosamine catabolic process; ISS:UniProtKB.
DR GO; GO:0006044; P:N-acetylglucosamine metabolic process; IEA:InterPro.
DR CDD; cd01399; GlcN6P_deaminase; 1.
DR HAMAP; MF_01241; GlcN6P_deamin; 1.
DR InterPro; IPR006148; Glc/Gal-6P_isomerase.
DR InterPro; IPR004547; Glucosamine6P_isomerase.
DR InterPro; IPR018321; Glucosamine6P_isomerase_CS.
DR InterPro; IPR037171; NagB/RpiA_transferase-like.
DR PANTHER; PTHR11280; PTHR11280; 1.
DR Pfam; PF01182; Glucosamine_iso; 1.
DR SUPFAM; SSF100950; SSF100950; 1.
DR TIGRFAMs; TIGR00502; nagB; 1.
DR PROSITE; PS01161; GLC_GALNAC_ISOMERASE; 1.
PE 3: Inferred from homology;
KW Alternative splicing; Carbohydrate metabolism; Cytoplasm; Hydrolase;
KW Reference proteome.
FT CHAIN 1..278
FT /note="Glucosamine-6-phosphate isomerase"
FT /id="PRO_0000328089"
FT ACT_SITE 72
FT /note="Proton acceptor; for enolization step"
FT /evidence="ECO:0000250"
FT ACT_SITE 141
FT /note="For ring-opening step"
FT /evidence="ECO:0000250"
FT ACT_SITE 143
FT /note="Proton acceptor; for ring-opening step"
FT /evidence="ECO:0000250"
FT ACT_SITE 148
FT /note="For ring-opening step"
FT /evidence="ECO:0000250"
FT VAR_SEQ 257..278
FT /note="ALSNVHHKLIEEGSTDVRKLCK -> SLYDVHSKLIEGT (in isoform
FT A)"
FT /evidence="ECO:0000305"
FT /id="VSP_032746"
FT VAR_SEQ 257..278
FT /note="ALSNVHHKLIEEGSTDVRKLCK -> DCYVLAAASGDLDDATRPN (in
FT isoform B)"
FT /evidence="ECO:0000305"
FT /id="VSP_032747"
SQ SEQUENCE 278 AA; 31400 MW; 7EE9426265D208BA CRC64;
MRLIILDTSD YVGEWSAKYV MKRINDFKPG PSRFFTLGLP TGSTPLGLYR NLIKFHQEGK
ISFKYVKTFN MDEYVDLPRD HPESYHYFMW HNFFKHIDID PVNVHILDGN APDLVAECNA
FEDKIKAAGG IELFIGGIGP DGHIAFNEPG SSLVSRTRVK TLAQDTLEAN ARFFGNDISK
VPKQALTVGV GTVMDAREVM ILIIGAHKAF ALYKAIEEGV NHMWTVSAFQ QHPHTIMICD
EDATLELRVK TVKYFKALSN VHHKLIEEGS TDVRKLCK
