ID   GNPI_CAEEL              Reviewed;         267 AA.
AC   Q9XVJ2;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1999, sequence version 1.
DT   03-AUG-2022, entry version 127.
DE   RecName: Full=Probable glucosamine-6-phosphate isomerase;
DE            EC=3.5.99.6;
DE   AltName: Full=Glucosamine-6-phosphate deaminase;
DE            Short=GNPDA;
DE            Short=GlcN6P deaminase;
GN   ORFNames=T03F6.3;
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=alpha-D-glucosamine 6-phosphate + H2O = beta-D-fructose 6-
CC         phosphate + NH4(+); Xref=Rhea:RHEA:12172, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:28938, ChEBI:CHEBI:57634, ChEBI:CHEBI:75989; EC=3.5.99.6;
CC   -!- SUBUNIT: Homohexamer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the glucosamine/galactosamine-6-phosphate
CC       isomerase family. {ECO:0000305}.
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DR   EMBL; Z81113; CAB03280.1; -; Genomic_DNA.
DR   PIR; T24397; T24397.
DR   RefSeq; NP_499758.1; NM_067357.4.
DR   AlphaFoldDB; Q9XVJ2; -.
DR   SMR; Q9XVJ2; -.
DR   BioGRID; 41929; 3.
DR   DIP; DIP-25269N; -.
DR   IntAct; Q9XVJ2; 1.
DR   STRING; 6239.T03F6.3; -.
DR   EPD; Q9XVJ2; -.
DR   PaxDb; Q9XVJ2; -.
DR   PeptideAtlas; Q9XVJ2; -.
DR   EnsemblMetazoa; T03F6.3.1; T03F6.3.1; WBGene00011399.
DR   GeneID; 176760; -.
DR   KEGG; cel:CELE_T03F6.3; -.
DR   UCSC; T03F6.3; c. elegans.
DR   CTD; 176760; -.
DR   WormBase; T03F6.3; CE16337; WBGene00011399; -.
DR   eggNOG; KOG3148; Eukaryota.
DR   GeneTree; ENSGT00390000014316; -.
DR   HOGENOM; CLU_049611_0_1_1; -.
DR   InParanoid; Q9XVJ2; -.
DR   OMA; FNEPCSS; -.
DR   OrthoDB; 1425290at2759; -.
DR   PhylomeDB; Q9XVJ2; -.
DR   Reactome; R-CEL-70171; Glycolysis.
DR   PRO; PR:Q9XVJ2; -.
DR   Proteomes; UP000001940; Chromosome III.
DR   Bgee; WBGene00011399; Expressed in germ line (C elegans) and 4 other tissues.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0004342; F:glucosamine-6-phosphate deaminase activity; IBA:GO_Central.
DR   GO; GO:0042802; F:identical protein binding; IBA:GO_Central.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0006043; P:glucosamine catabolic process; IBA:GO_Central.
DR   GO; GO:0006046; P:N-acetylglucosamine catabolic process; IBA:GO_Central.
DR   GO; GO:0019262; P:N-acetylneuraminate catabolic process; IBA:GO_Central.
DR   CDD; cd01399; GlcN6P_deaminase; 1.
DR   HAMAP; MF_01241; GlcN6P_deamin; 1.
DR   InterPro; IPR006148; Glc/Gal-6P_isomerase.
DR   InterPro; IPR004547; Glucosamine6P_isomerase.
DR   InterPro; IPR018321; Glucosamine6P_isomerase_CS.
DR   InterPro; IPR037171; NagB/RpiA_transferase-like.
DR   PANTHER; PTHR11280; PTHR11280; 1.
DR   Pfam; PF01182; Glucosamine_iso; 1.
DR   SUPFAM; SSF100950; SSF100950; 1.
DR   TIGRFAMs; TIGR00502; nagB; 1.
DR   PROSITE; PS01161; GLC_GALNAC_ISOMERASE; 1.
PE   3: Inferred from homology;
KW   Carbohydrate metabolism; Cytoplasm; Hydrolase; Reference proteome.
FT   CHAIN           1..267
FT                   /note="Probable glucosamine-6-phosphate isomerase"
FT                   /id="PRO_0000160126"
FT   ACT_SITE        71
FT                   /note="Proton acceptor; for enolization step"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        140
FT                   /note="For ring-opening step"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        142
FT                   /note="Proton acceptor; for ring-opening step"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        147
FT                   /note="For ring-opening step"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   267 AA;  29913 MW;  A0A30B74D7B253CB CRC64;
     MKLIIEENAD KVAEFAARYV VTKINEATEN GKYLVLGLPT GSTPLGMYKK LIEFYNAGVI
     SFEKVKTFNM DEYVDLPRDH TESYHSFMFD NFFRHIDINP ANIHILDGNT SDHEKECEEY
     ERKIKESGGI DLFVGGIGPD GHIAFNEPGS SLASRTRIKT LNEDTIQANA RFFGGDITKV
     PTQALTVGVQ TVMDAREVMI LITGSHKALA LHQAIECGIS HMCTVSAMQM HRCATFIADE
     DATLELKVKT VKYFKGLMNH HRSLVMF