ID   GNPI_DICDI              Reviewed;         267 AA.
AC   Q54XK9;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 96.
DE   RecName: Full=Glucosamine-6-phosphate isomerase;
DE            EC=3.5.99.6;
DE   AltName: Full=Glucosamine-6-phosphate deaminase;
DE            Short=GNPDA;
DE            Short=GlcN6P deaminase;
GN   Name=gnpda1; Synonyms=gniA, nagB2; ORFNames=DDB_G0278873;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=alpha-D-glucosamine 6-phosphate + H2O = beta-D-fructose 6-
CC         phosphate + NH4(+); Xref=Rhea:RHEA:12172, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:28938, ChEBI:CHEBI:57634, ChEBI:CHEBI:75989; EC=3.5.99.6;
CC   -!- SUBUNIT: Homohexamer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the glucosamine/galactosamine-6-phosphate
CC       isomerase family. {ECO:0000305}.
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DR   EMBL; AAFI02000024; EAL68038.1; -; Genomic_DNA.
DR   RefSeq; XP_647794.1; XM_642702.1.
DR   AlphaFoldDB; Q54XK9; -.
DR   SMR; Q54XK9; -.
DR   STRING; 44689.DDB0234126; -.
DR   PaxDb; Q54XK9; -.
DR   EnsemblProtists; EAL68038; EAL68038; DDB_G0278873.
DR   GeneID; 8621753; -.
DR   KEGG; ddi:DDB_G0278873; -.
DR   dictyBase; DDB_G0278873; gnpda1.
DR   eggNOG; KOG3148; Eukaryota.
DR   HOGENOM; CLU_049611_0_1_1; -.
DR   InParanoid; Q54XK9; -.
DR   OMA; FNEPCSS; -.
DR   PhylomeDB; Q54XK9; -.
DR   Reactome; R-DDI-70171; Glycolysis.
DR   PRO; PR:Q54XK9; -.
DR   Proteomes; UP000002195; Chromosome 3.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0004342; F:glucosamine-6-phosphate deaminase activity; ISS:UniProtKB.
DR   GO; GO:0042802; F:identical protein binding; IBA:GO_Central.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0006091; P:generation of precursor metabolites and energy; ISS:UniProtKB.
DR   GO; GO:0006043; P:glucosamine catabolic process; ISS:UniProtKB.
DR   GO; GO:0006046; P:N-acetylglucosamine catabolic process; IBA:GO_Central.
DR   GO; GO:0019262; P:N-acetylneuraminate catabolic process; IBA:GO_Central.
DR   CDD; cd01399; GlcN6P_deaminase; 1.
DR   HAMAP; MF_01241; GlcN6P_deamin; 1.
DR   InterPro; IPR006148; Glc/Gal-6P_isomerase.
DR   InterPro; IPR004547; Glucosamine6P_isomerase.
DR   InterPro; IPR018321; Glucosamine6P_isomerase_CS.
DR   InterPro; IPR037171; NagB/RpiA_transferase-like.
DR   PANTHER; PTHR11280; PTHR11280; 1.
DR   Pfam; PF01182; Glucosamine_iso; 1.
DR   SUPFAM; SSF100950; SSF100950; 1.
DR   TIGRFAMs; TIGR00502; nagB; 1.
DR   PROSITE; PS01161; GLC_GALNAC_ISOMERASE; 1.
PE   3: Inferred from homology;
KW   Carbohydrate metabolism; Cytoplasm; Hydrolase; Reference proteome.
FT   CHAIN           1..267
FT                   /note="Glucosamine-6-phosphate isomerase"
FT                   /id="PRO_0000328093"
FT   ACT_SITE        76
FT                   /note="Proton acceptor; for enolization step"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        145
FT                   /note="For ring-opening step"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        147
FT                   /note="Proton acceptor; for ring-opening step"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        152
FT                   /note="For ring-opening step"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   267 AA;  30571 MW;  99E3CE06E78FB9A9 CRC64;
     MRLIIKNDSC EAGEYISQVI KKRINDYFLN RKSGDNRPFV IGLPTGSSPL PIYKRLIEMN
     KNKEISFQEV VTFNMDEYVG LESNHKFSYH YFMWENFFNH IDIKKENINI LNGTTDNHEN
     ECKLYEEKIQ SYGGIDLFLG GMGVDGHIAF NEPGSSLSSR TRIKTLTRDT IIANSRFFNN
     INQVPTQALT VGVGTIMDAR EIILIVTGHS KAIALYRTIE EGVNHMWTAS AIQMHKKSII
     VCDEDATAEL KVKTYKYFKQ VESNSNY