GNPI_DROME
ID GNPI_DROME Reviewed; 273 AA.
AC Q9VMP9;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Glucosamine-6-phosphate isomerase;
DE EC=3.5.99.6;
DE AltName: Full=Glucosamine-6-phosphate deaminase;
DE Short=GNPDA;
DE Short=GlcN6P deaminase;
DE AltName: Full=Oscillin homolog;
GN Name=Oscillin; Synonyms=Gnpda1; ORFNames=CG6957;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Larva, and Pupae;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP IDENTIFICATION.
RX PubMed=10964516; DOI=10.1006/geno.2000.6287;
RA Nakamura Y., Miura K., Fujino Y., Iwao H., Ogita S., Yamanaka S.;
RT "Evolution, structure, and expression of GNPI/Oscillin orthologous genes.";
RL Genomics 68:179-186(2000).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=alpha-D-glucosamine 6-phosphate + H2O = beta-D-fructose 6-
CC phosphate + NH4(+); Xref=Rhea:RHEA:12172, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:28938, ChEBI:CHEBI:57634, ChEBI:CHEBI:75989; EC=3.5.99.6;
CC -!- SUBUNIT: Homohexamer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the glucosamine/galactosamine-6-phosphate
CC isomerase family. {ECO:0000305}.
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DR EMBL; AE014134; AAF52263.1; -; Genomic_DNA.
DR EMBL; AE014134; AAN10546.1; -; Genomic_DNA.
DR EMBL; AY122220; AAM52732.1; -; mRNA.
DR RefSeq; NP_001245894.1; NM_001258965.2.
DR RefSeq; NP_001245897.1; NM_001258968.1.
DR RefSeq; NP_001260101.1; NM_001273172.1.
DR RefSeq; NP_608938.1; NM_135094.2.
DR RefSeq; NP_723092.1; NM_164649.2.
DR AlphaFoldDB; Q9VMP9; -.
DR SMR; Q9VMP9; -.
DR BioGRID; 59951; 7.
DR IntAct; Q9VMP9; 1.
DR STRING; 7227.FBpp0297903; -.
DR PaxDb; Q9VMP9; -.
DR DNASU; 33783; -.
DR EnsemblMetazoa; FBtr0079101; FBpp0078734; FBgn0031717.
DR EnsemblMetazoa; FBtr0079102; FBpp0078735; FBgn0031717.
DR EnsemblMetazoa; FBtr0307060; FBpp0297903; FBgn0031717.
DR EnsemblMetazoa; FBtr0307063; FBpp0297906; FBgn0031717.
DR EnsemblMetazoa; FBtr0331675; FBpp0304065; FBgn0031717.
DR GeneID; 33783; -.
DR KEGG; dme:Dmel_CG6957; -.
DR UCSC; CG6957-RA; d. melanogaster.
DR CTD; 33783; -.
DR FlyBase; FBgn0031717; Oscillin.
DR VEuPathDB; VectorBase:FBgn0031717; -.
DR eggNOG; KOG3148; Eukaryota.
DR GeneTree; ENSGT00390000014316; -.
DR InParanoid; Q9VMP9; -.
DR OMA; FNEPCSS; -.
DR PhylomeDB; Q9VMP9; -.
DR Reactome; R-DME-70171; Glycolysis.
DR BioGRID-ORCS; 33783; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 33783; -.
DR PRO; PR:Q9VMP9; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0031717; Expressed in saliva-secreting gland and 29 other tissues.
DR ExpressionAtlas; Q9VMP9; baseline and differential.
DR Genevisible; Q9VMP9; DM.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0004342; F:glucosamine-6-phosphate deaminase activity; ISS:UniProtKB.
DR GO; GO:0042802; F:identical protein binding; IBA:GO_Central.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0006091; P:generation of precursor metabolites and energy; ISS:UniProtKB.
DR GO; GO:0006043; P:glucosamine catabolic process; ISS:UniProtKB.
DR GO; GO:0006046; P:N-acetylglucosamine catabolic process; IBA:GO_Central.
DR GO; GO:0006044; P:N-acetylglucosamine metabolic process; ISS:FlyBase.
DR GO; GO:0019262; P:N-acetylneuraminate catabolic process; IBA:GO_Central.
DR CDD; cd01399; GlcN6P_deaminase; 1.
DR HAMAP; MF_01241; GlcN6P_deamin; 1.
DR InterPro; IPR006148; Glc/Gal-6P_isomerase.
DR InterPro; IPR004547; Glucosamine6P_isomerase.
DR InterPro; IPR018321; Glucosamine6P_isomerase_CS.
DR InterPro; IPR037171; NagB/RpiA_transferase-like.
DR PANTHER; PTHR11280; PTHR11280; 1.
DR Pfam; PF01182; Glucosamine_iso; 1.
DR SUPFAM; SSF100950; SSF100950; 1.
DR TIGRFAMs; TIGR00502; nagB; 1.
DR PROSITE; PS01161; GLC_GALNAC_ISOMERASE; 1.
PE 2: Evidence at transcript level;
KW Carbohydrate metabolism; Cytoplasm; Hydrolase; Reference proteome.
FT CHAIN 1..273
FT /note="Glucosamine-6-phosphate isomerase"
FT /id="PRO_0000328091"
FT ACT_SITE 72
FT /note="Proton acceptor; for enolization step"
FT /evidence="ECO:0000250"
FT ACT_SITE 141
FT /note="For ring-opening step"
FT /evidence="ECO:0000250"
FT ACT_SITE 143
FT /note="Proton acceptor; for ring-opening step"
FT /evidence="ECO:0000250"
FT ACT_SITE 148
FT /note="For ring-opening step"
FT /evidence="ECO:0000250"
SQ SEQUENCE 273 AA; 30824 MW; BE351A15C652E955 CRC64;
MRLVILETSD SVGKWAAKYV MKRINDFQPS ADRYFVLGLP TGSTPLGMYK ELIEFHKQGK
VSFQYVKTFN MDEYVGLARD HHESYHYFMW NNFFKHIDIE PKNVHILDGN AADLVAECNK
FEDQIREAGG VELFIGGIGP DGHIAFNEPG SSLVSRTRVK TLAQDTLEAN ARFFDNDMSK
VPKQALTVGV GTVMDSKEVM ILITGAHKAF ALYKAIEEGV NHMWTVSAFQ QHANTLMICD
EDATLELRVK TVKYFKGILR DLDEGSPLEG YKN
