GNR1_SCHPO
ID GNR1_SCHPO Reviewed; 399 AA.
AC O59762;
DT 01-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 25-MAY-2022, entry version 135.
DE RecName: Full=Guanine nucleotide-binding protein negative regulator 1;
DE Short=G protein negative regulator 1;
DE AltName: Full=WD repeat-containing protein gnr1;
GN Name=gnr1; ORFNames=SPCC1020.09;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP FUNCTION, AND INTERACTION WITH GPA1.
RX PubMed=16884933; DOI=10.1016/j.fgb.2006.06.005;
RA Goddard A., Ladds G., Forfar R., Davey J.;
RT "Identification of Gnr1p, a negative regulator of G alpha signalling in
RT Schizosaccharomyces pombe, and its complementation by human G beta
RT subunits.";
RL Fungal Genet. Biol. 43:840-851(2006).
RN [3]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
CC -!- FUNCTION: Negatively regulates the pheromone-response pathway. Acts as
CC a structural mimic of the G protein beta subunit thereby interacting
CC with gpa1 which then inhibits gpa1 signaling.
CC {ECO:0000269|PubMed:16884933}.
CC -!- SUBUNIT: Interacts with gpa1. {ECO:0000269|PubMed:16884933}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16823372}.
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DR EMBL; CU329672; CAA18997.1; -; Genomic_DNA.
DR PIR; T40831; T40831.
DR RefSeq; NP_587950.1; NM_001022941.2.
DR AlphaFoldDB; O59762; -.
DR SMR; O59762; -.
DR BioGRID; 275871; 26.
DR STRING; 4896.SPCC1020.09.1; -.
DR MaxQB; O59762; -.
DR PaxDb; O59762; -.
DR EnsemblFungi; SPCC1020.09.1; SPCC1020.09.1:pep; SPCC1020.09.
DR GeneID; 2539303; -.
DR KEGG; spo:SPCC1020.09; -.
DR PomBase; SPCC1020.09; gnr1.
DR VEuPathDB; FungiDB:SPCC1020.09; -.
DR eggNOG; KOG2919; Eukaryota.
DR HOGENOM; CLU_691098_0_0_1; -.
DR InParanoid; O59762; -.
DR OMA; THTAGPQ; -.
DR PhylomeDB; O59762; -.
DR PRO; PR:O59762; -.
DR Proteomes; UP000002485; Chromosome III.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0005834; C:heterotrimeric G-protein complex; IPI:PomBase.
DR GO; GO:0043409; P:negative regulation of MAPK cascade; IMP:PomBase.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR SMART; SM00320; WD40; 6.
DR SUPFAM; SSF50978; SSF50978; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Reference proteome; Repeat; WD repeat.
FT CHAIN 1..399
FT /note="Guanine nucleotide-binding protein negative
FT regulator 1"
FT /id="PRO_0000343431"
FT REPEAT 44..83
FT /note="WD 1"
FT REPEAT 105..145
FT /note="WD 2"
FT REPEAT 150..194
FT /note="WD 3"
FT REPEAT 207..247
FT /note="WD 4"
FT REPEAT 252..292
FT /note="WD 5"
FT REPEAT 296..337
FT /note="WD 6"
SQ SEQUENCE 399 AA; 45747 MW; 8F779B1AACFA00FD CRC64;
MDNCVNSFED QKDDLVHKKK SQNFGYVCGS INLGTNVIAQ SPTKPLNFFH SSRWSPDGST
ILSLTEDQCL NCWNVPFSDL SKKADGPLNF SKHLSYKYQS PETVYSYSWY SRMKLDDPSS
NLFAVSSRDQ PIKLINFTTG KNKASYHMID HQERYQGSHC LQFTNDGEYL IAGDKNCLHH
FNIRTGCKEP VMTTVTHGYK VPLWEFSLKG IQSCFSLNPM DSKTLAVGTY SNRVGIYNDC
GRRPCQLEFS IERGNGVTHL QWCEDGEKLY VGSRCSDKIE VWDIRYVRDM VYALEGHRGD
TNQRILFDTD KKDEILAGGT DGSIRRWRNK DLVEETHVTG NYDLTVNTVQ ANPINMQIKC
VCYGNRIYKY EKDESEEEDE SKEKDLWTGT VSALQVWMD
