GNRA_DICDI
ID GNRA_DICDI Reviewed; 1087 AA.
AC O96923; Q54F43;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 25-MAY-2022, entry version 96.
DE RecName: Full=Gelsolin-related protein of 125 kDa;
DE Short=GRP125;
GN Name=gnrA; ORFNames=DDB_G0291125;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], DEVELOPMENTAL STAGE, DISRUPTION
RP PHENOTYPE, FUNCTION, AND SUBCELLULAR LOCATION.
RC STRAIN=AX2;
RX PubMed=9880334; DOI=10.1091/mbc.10.1.161;
RA Stocker S., Hiery M., Marriott G.;
RT "Phototactic migration of Dictyostelium cells is linked to a new type of
RT gelsolin-related protein.";
RL Mol. Biol. Cell 10:161-178(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3]
RP INTERACTION WITH RASD AND ABPC.
RX PubMed=16735078; DOI=10.1016/j.ejcb.2006.04.005;
RA Bandala-Sanchez E., Annesley S.J., Fisher P.R.;
RT "A phototaxis signalling complex in Dictyostelium discoideum.";
RL Eur. J. Cell Biol. 85:1099-1106(2006).
CC -!- FUNCTION: Involved in phototaxis. Required for coupling photodetection
CC to the locomotory machinery of slugs. May be essential in the natural
CC environment for the propagation of spores.
CC {ECO:0000269|PubMed:9880334}.
CC -!- SUBUNIT: Interacts with rasD and abpC. {ECO:0000269|PubMed:16735078}.
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle {ECO:0000269|PubMed:9880334}.
CC -!- DEVELOPMENTAL STAGE: Expression is tightly regulated. Barely present in
CC vegetative cells and peaks at the transition to the multicellular
CC pseudoplasmodial stage of development. {ECO:0000269|PubMed:9880334}.
CC -!- DISRUPTION PHENOTYPE: Severe defect in the ability of slugs to orient
CC properly towards the light. {ECO:0000269|PubMed:9880334}.
CC -!- SIMILARITY: Belongs to the villin/gelsolin family. {ECO:0000305}.
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DR EMBL; U95159; AAD00774.1; -; Genomic_DNA.
DR EMBL; AAFI02000175; EAL61846.1; -; Genomic_DNA.
DR PIR; T30330; T30330.
DR RefSeq; XP_635347.1; XM_630255.1.
DR AlphaFoldDB; O96923; -.
DR SMR; O96923; -.
DR STRING; 44689.DDB0191398; -.
DR PaxDb; O96923; -.
DR PRIDE; O96923; -.
DR EnsemblProtists; EAL61846; EAL61846; DDB_G0291125.
DR GeneID; 8627995; -.
DR KEGG; ddi:DDB_G0291125; -.
DR dictyBase; DDB_G0291125; gnrA.
DR eggNOG; KOG0443; Eukaryota.
DR HOGENOM; CLU_285204_0_0_1; -.
DR InParanoid; O96923; -.
DR OMA; FILDCEH; -.
DR PhylomeDB; O96923; -.
DR Reactome; R-DDI-6798695; Neutrophil degranulation.
DR PRO; PR:O96923; -.
DR Proteomes; UP000002195; Chromosome 5.
DR GO; GO:0015629; C:actin cytoskeleton; IBA:GO_Central.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0031410; C:cytoplasmic vesicle; IDA:dictyBase.
DR GO; GO:0051015; F:actin filament binding; IBA:GO_Central.
DR GO; GO:0008154; P:actin polymerization or depolymerization; IBA:GO_Central.
DR GO; GO:0042331; P:phototaxis; IMP:dictyBase.
DR Gene3D; 3.40.20.10; -; 5.
DR InterPro; IPR029006; ADF-H/Gelsolin-like_dom_sf.
DR InterPro; IPR007123; Gelsolin-like_dom.
DR InterPro; IPR007122; Villin/Gelsolin.
DR PANTHER; PTHR11977; PTHR11977; 1.
DR Pfam; PF00626; Gelsolin; 3.
DR PRINTS; PR00597; GELSOLIN.
DR SMART; SM00262; GEL; 5.
PE 1: Evidence at protein level;
KW Coiled coil; Cytoplasmic vesicle; Reference proteome; Repeat.
FT CHAIN 1..1087
FT /note="Gelsolin-related protein of 125 kDa"
FT /id="PRO_0000384375"
FT REPEAT 73..146
FT /note="Gelsolin-like 1"
FT REPEAT 183..286
FT /note="Gelsolin-like 2"
FT REPEAT 335..442
FT /note="Gelsolin-like 3"
FT REPEAT 465..538
FT /note="Gelsolin-like 4"
FT REPEAT 614..692
FT /note="Gelsolin-like 5"
FT REGION 1..40
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 555..592
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 732..1087
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 550..598
FT /evidence="ECO:0000255"
FT COILED 710..912
FT /evidence="ECO:0000255"
FT COMPBIAS 8..24
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 25..40
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 563..586
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 732..765
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 777..841
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 848..900
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 921..938
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 983..998
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1002..1067
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1068..1087
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1087 AA; 124778 MW; 8C431F5A0B51E677 CRC64;
MEEDNIVDSK EIENNVEDKK EETPSSSPSP SSSLQQQQEE GGVVKQAVSN LVSSSITLHD
DAKLIQVTGK DEPFHFISVP MSVEFLNLED VFIMQSDAYI FVWCSEQANI KKKAKAVQMA
QKLKVEIGCQ RAVQVLEIGE EHPTFLFCLG VPKGTKLNVT KEKNDIFQVD EDDEEQVLEP
EFFLFKIFTG TDGKPSIKPM EEDEGINQEM LESSACFILD CEHEMYIWLG KGVKKSTKDT
LIPVAKKIWT QYDRPEYYGK LKLQPIITWV FDGAESCLFK SKFSKWVEKA QPLQTSYLSL
SSKKKEALNF DVSSMHQDKE VPVINIGAAS DYSNGKLLVW CSGGGSGNKW NKVEEDDFGI
FYSNKSYVCH FIYKPENKNS IRSVIFFWEG LYSNQRNYIG YKFGLYKEIQ KKMEGLKSDD
PVEYRISQNK EPQEFINLFG TELLVLNEEL ALKPMIFQVR ANRGTQLFPD PDSCNAKLLN
SLDSFVFLFP NKYIIVWHGK VSTEHQRELA ADLFTFLPPE YEAGVKEFDQ GKESDNFWKI
IGGNSNDIVI NTFINENKEE KEKEEEEKEE EEEEEEEEEE EEEEEKDNNK TTTIIKHLRP
KKIKLFLCTD NSGIFKADQI NPFSQVDLNS QECVLLDVYH KVFLWKGSKS TDQKLNDTQD
LAKQYIETAN DQRPSDCSVE LVEQYNESPL FKSYFHSWKV TPPKVFIDPI ISYKQKLAER
LQKEKEDLEK LKQQQEQEQE QQQKENNKIV EEVKEEVKEE DVKEEVKEEE VKEEEVKEEE
VKEVAKEETK EEIKEEVNDE ATEVKEVNQV EEEVKEEEVK EEVKVEVKEE EVKGEAKEEE
VKEEEVKEEE VKEEVKEVKE EVKEEVKQDK EEEVNEEIKE ETKEEETKED DNKEDEKVNE
ENETVNEENE VGIIVSPPSE KVDEANSSST ISSPENEGSV SVKDKRKSNE PITPSVVSSS
GDLLFAYEPY HGPPIHSSNS PKQGRKGGRK SHGKNQPQHK KNHSVDQSPL SSPIIPNKSL
NLDIDNQSFD LNSINNNNSV EVLDGASSPL SFSSSSINSN STHNTPSKKN KNKNKKKHNR
SSSLTHA
