ID   GNSA_ECOLI              Reviewed;          57 AA.
AC   P0AC92; P52635; Q2EER1;
DT   08-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   08-NOV-2005, sequence version 1.
DT   03-AUG-2022, entry version 94.
DE   RecName: Full=Protein GnsA;
GN   Name=gnsA; Synonyms=yccL; OrderedLocusNames=b4517, JW0976;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12;
RX   PubMed=8808925; DOI=10.1128/jb.178.18.5382-5387.1996;
RA   Rock C.O., Tsay J.-T., Heath R., Jackowski S.;
RT   "Increased unsaturated fatty acid production associated with a suppressor
RT   of the fabA6(Ts) mutation in Escherichia coli.";
RL   J. Bacteriol. 178:5382-5387(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA   Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA   Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA   Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA   Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA   Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA   Horiuchi T.;
RT   "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT   the 12.7-28.0 min region on the linkage map.";
RL   DNA Res. 3:137-155(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [5]
RP   FUNCTION.
RX   PubMed=11544213; DOI=10.1128/jb.183.19.5523-5528.2001;
RA   Sugai R., Shimizu H., Nishiyama K., Tokuda H.;
RT   "Overexpression of yccL (gnsA) and ydfY (gnsB) increases levels of
RT   unsaturated fatty acids and suppresses both the temperature-sensitive fabA6
RT   mutation and cold-sensitive secG null mutation of Escherichia coli.";
RL   J. Bacteriol. 183:5523-5528(2001).
CC   -!- FUNCTION: Overexpression increases levels of unsaturated fatty acids
CC       and suppresses both the temperature-sensitive fabA6 mutation and cold-
CC       sensitive secG null mutation. {ECO:0000269|PubMed:11544213}.
CC   -!- INTERACTION:
CC       P0AC92; P0AC92: gnsA; NbExp=2; IntAct=EBI-1121356, EBI-1121356;
CC   -!- SIMILARITY: Belongs to the gns family. {ECO:0000305}.
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DR   EMBL; U38541; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; U00096; ABD18647.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA35758.1; -; Genomic_DNA.
DR   RefSeq; WP_001019197.1; NZ_STEB01000006.1.
DR   RefSeq; YP_588446.1; NC_000913.3.
DR   PDB; 4XO1; X-ray; 1.80 A; A=1-57.
DR   PDB; 4XO2; X-ray; 1.95 A; A/B=1-57.
DR   PDBsum; 4XO1; -.
DR   PDBsum; 4XO2; -.
DR   AlphaFoldDB; P0AC92; -.
DR   SMR; P0AC92; -.
DR   BioGRID; 4262837; 14.
DR   BioGRID; 853386; 3.
DR   IntAct; P0AC92; 9.
DR   STRING; 511145.b4517; -.
DR   jPOST; P0AC92; -.
DR   PaxDb; P0AC92; -.
DR   PRIDE; P0AC92; -.
DR   EnsemblBacteria; ABD18647; ABD18647; b4517.
DR   EnsemblBacteria; BAA35758; BAA35758; BAA35758.
DR   GeneID; 1450249; -.
DR   GeneID; 66670731; -.
DR   KEGG; ecj:JW0976; -.
DR   KEGG; eco:b4517; -.
DR   PATRIC; fig|1411691.4.peg.1280; -.
DR   EchoBASE; EB3022; -.
DR   eggNOG; ENOG50332DR; Bacteria.
DR   HOGENOM; CLU_197432_0_0_6; -.
DR   PhylomeDB; P0AC92; -.
DR   BioCyc; EcoCyc:MON0-1701; -.
DR   PRO; PR:P0AC92; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0005829; C:cytosol; IDA:EcoCyc.
DR   GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR   InterPro; IPR012563; Gns.
DR   Pfam; PF08178; GnsAB_toxin; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Reference proteome.
FT   CHAIN           1..57
FT                   /note="Protein GnsA"
FT                   /id="PRO_0000201722"
FT   HELIX           3..29
FT                   /evidence="ECO:0007829|PDB:4XO1"
FT   STRAND          30..43
FT                   /evidence="ECO:0007829|PDB:4XO1"
FT   STRAND          46..57
FT                   /evidence="ECO:0007829|PDB:4XO1"
SQ   SEQUENCE   57 AA;  6576 MW;  7BD48340D6D2966C CRC64;
     MNIEELKKQA ETEIADFIAQ KIAELNKNTG KEVSEIRFTA REKMTGLESY DVKIKIM