GNT15_DICDI
ID GNT15_DICDI Reviewed; 516 AA.
AC Q555X4;
DT 20-APR-2010, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 25-MAY-2022, entry version 67.
DE RecName: Full=Glycosyltransferase-like protein gnt15;
GN Name=gnt15; ORFNames=DDB_G0274741;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=12097910; DOI=10.1038/nature00847;
RA Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA Noegel A.A.;
RT "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL Nature 418:79-85(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3]
RP DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE, AND FUNCTION.
RX PubMed=17588537; DOI=10.1016/j.bbrc.2007.06.016;
RA Pang T.L., Wu C.J., Chen P.A., Weng Y.L., Chen M.Y.;
RT "Dictyostelium gnt15 encodes a protein with similarity to LARGE and plays
RT an essential role in development.";
RL Biochem. Biophys. Res. Commun. 360:83-89(2007).
CC -!- FUNCTION: May have a role in modulating cell adhesion and
CC glycosylation. Essential for development.
CC {ECO:0000269|PubMed:17588537}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type II
CC membrane protein {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: Highest expression at vegetative and pre-
CC aggregation stages. {ECO:0000269|PubMed:17588537}.
CC -!- DISRUPTION PHENOTYPE: Slow growth, aberrant morphology and development.
CC Produces a severe defect in spore formation. Cells are more adhesive.
CC Alterations in glycosylation on membrane proteins and decreased gp130
CC and gp150 levels are observed. {ECO:0000269|PubMed:17588537}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 8 family. Highly
CC divergent. {ECO:0000305}.
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DR EMBL; AAFI02000012; EAL70263.1; -; Genomic_DNA.
DR RefSeq; XP_643936.1; XM_638844.1.
DR AlphaFoldDB; Q555X4; -.
DR PaxDb; Q555X4; -.
DR EnsemblProtists; EAL70263; EAL70263; DDB_G0274741.
DR GeneID; 8619363; -.
DR KEGG; ddi:DDB_G0274741; -.
DR dictyBase; DDB_G0274741; gnt15.
DR eggNOG; KOG3765; Eukaryota.
DR HOGENOM; CLU_528327_0_0_1; -.
DR InParanoid; Q555X4; -.
DR PhylomeDB; Q555X4; -.
DR PRO; PR:Q555X4; -.
DR Proteomes; UP000002195; Chromosome 2.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015020; F:glucuronosyltransferase activity; IBA:GO_Central.
DR GO; GO:0042285; F:xylosyltransferase activity; IBA:GO_Central.
DR GO; GO:0016338; P:calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules; IMP:dictyBase.
DR GO; GO:0035269; P:protein O-linked mannosylation; IBA:GO_Central.
DR GO; GO:0030587; P:sorocarp development; IMP:dictyBase.
DR Gene3D; 3.90.550.10; -; 1.
DR InterPro; IPR029044; Nucleotide-diphossugar_trans.
PE 2: Evidence at transcript level;
KW Glycoprotein; Membrane; Reference proteome; Signal-anchor; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..516
FT /note="Glycosyltransferase-like protein gnt15"
FT /id="PRO_0000393410"
FT TOPO_DOM 1..24
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 25..45
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 46..516
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT REGION 199..250
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 199..247
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 152
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 386
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 412
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 516 AA; 59898 MW; 491B655C3D1BB935 CRC64;
MSNFYNNNPR RNTFRLTERI KKKPYQTLIV FILIFLFLYV FGPFGEKKSN NNNNNHPVSK
TSSFTESLYT KFQTETFAYR ANGDLKKYDI SIITQFTVDR FDRIAMMADK WRAPISAAVY
ITSFKDIDEV FKLVRNSFAV TEFVDLHFLF ANKTRYPVNN LRNLALRNAR TEWCLLLDVD
FISPLGMYDY LHSTLEKLDT SNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNENNDNDNG
NNNNNNDNEK NFKKKQEDLK IDPNDFEGDL KAIEKLKRNG EKLYVKNLKK VLDGSENNLG
KNINFNNNNN DNNNKDDGGG GGYYLNSDNS NINNNNKIAF VIPSFSSSIS RFDFPDNKKD
LLDFIKQDLI KEINSGVCPK CHGPTNYSRW YLSSEPYLVQ YKWIYEPFLL YNRSQIHDYD
ERLKGYGFDK NSHTFGMAAA GFDFVVLPDA WIIHMNHVSK PWEGADTFNE QMFDCLSIVC
ESILPDAKSK NGYDPNAKLF NEPLKNNDNC LTREHW
