GNT1_YEAST
ID GNT1_YEAST Reviewed; 491 AA.
AC Q12096; D6W318;
DT 25-OCT-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Glucose N-acetyltransferase 1;
DE EC=2.4.1.-;
DE AltName: Full=N-acetylglucosaminyltransferase;
GN Name=GNT1; OrderedLocusNames=YOR320C; ORFNames=O6145;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 96604 / S288c / FY1679;
RX PubMed=8896266;
RX DOI=10.1002/(sici)1097-0061(199609)12:10b<1021::aid-yea981>3.0.co;2-7;
RA Pearson B.M., Hernando Y., Payne J., Wolf S.S., Kalogeropoulos A.,
RA Schweizer M.;
RT "Sequencing of a 35.71 kb DNA segment on the right arm of yeast chromosome
RT XV reveals regions of similarity to chromosomes I and XIII.";
RL Yeast 12:1021-1031(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169874;
RA Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J.,
RA Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A.,
RA Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B.,
RA Dang V.-D., de Haan M., Delius H., Durand P., Fairhead C., Feldmann H.,
RA Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E.,
RA Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U.,
RA Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B.,
RA Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A.,
RA Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C.,
RA Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G.,
RA Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B.,
RA Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B.,
RA Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F.,
RA Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E.,
RA Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I.,
RA Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H.,
RA Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.";
RL Nature 387:98-102(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP FUNCTION, GLYCOSYLATION, AND SUBCELLULAR LOCATION.
RX PubMed=12651885; DOI=10.1093/glycob/cwg063;
RA Yoko-o T., Wiggins C.A.R., Stolz J., Peak-Chew S.Y., Munro S.;
RT "An N-acetylglucosaminyltransferase of the Golgi apparatus of the yeast
RT Saccharomyces cerevisiae that can modify N-linked glycans.";
RL Glycobiology 13:581-589(2003).
RN [5]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [7]
RP SUBCELLULAR LOCATION, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=16107716; DOI=10.1128/mcb.25.17.7696-7710.2005;
RA Inadome H., Noda Y., Adachi H., Yoda K.;
RT "Immunoisolation of the yeast Golgi subcompartments and characterization of
RT a novel membrane protein, Svp26, discovered in the Sed5-containing
RT compartments.";
RL Mol. Cell. Biol. 25:7696-7710(2005).
CC -!- FUNCTION: N-acetylglucosaminyltransferase involved in the Golgi-
CC specific modification of N-linked glycans.
CC {ECO:0000269|PubMed:12651885}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000305}; Single-
CC pass type II membrane protein {ECO:0000305}. Vacuole membrane
CC {ECO:0000305}; Single-pass type II membrane protein {ECO:0000305}.
CC -!- PTM: N-glycosylated. {ECO:0000269|PubMed:12651885}.
CC -!- MISCELLANEOUS: Present with 238 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the GNT1 family. {ECO:0000305}.
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DR EMBL; X90565; CAA62175.1; -; Genomic_DNA.
DR EMBL; Z75228; CAA99640.1; -; Genomic_DNA.
DR EMBL; BK006948; DAA11084.1; -; Genomic_DNA.
DR PIR; S58330; S58330.
DR RefSeq; NP_014965.3; NM_001183740.3.
DR AlphaFoldDB; Q12096; -.
DR BioGRID; 34706; 62.
DR DIP; DIP-2557N; -.
DR STRING; 4932.YOR320C; -.
DR CAZy; GT8; Glycosyltransferase Family 8.
DR MaxQB; Q12096; -.
DR PaxDb; Q12096; -.
DR PRIDE; Q12096; -.
DR EnsemblFungi; YOR320C_mRNA; YOR320C; YOR320C.
DR GeneID; 854498; -.
DR KEGG; sce:YOR320C; -.
DR SGD; S000005847; GNT1.
DR VEuPathDB; FungiDB:YOR320C; -.
DR eggNOG; KOG1950; Eukaryota.
DR HOGENOM; CLU_034860_1_0_1; -.
DR InParanoid; Q12096; -.
DR OMA; ILPHRVY; -.
DR BioCyc; YEAST:G3O-33800-MON; -.
DR Reactome; R-SCE-3322077; Glycogen synthesis.
DR Reactome; R-SCE-6798695; Neutrophil degranulation.
DR Reactome; R-SCE-70221; Glycogen breakdown (glycogenolysis).
DR PRO; PR:Q12096; -.
DR Proteomes; UP000002311; Chromosome XV.
DR RNAct; Q12096; protein.
DR GO; GO:0005797; C:Golgi medial cisterna; IDA:SGD.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008375; F:acetylglucosaminyltransferase activity; IDA:SGD.
DR GO; GO:0016757; F:glycosyltransferase activity; IBA:GO_Central.
DR GO; GO:0006487; P:protein N-linked glycosylation; IMP:SGD.
DR Gene3D; 3.90.550.10; -; 1.
DR InterPro; IPR030518; GNT1.
DR InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR PANTHER; PTHR11183:SF121; PTHR11183:SF121; 1.
DR SUPFAM; SSF53448; SSF53448; 1.
PE 1: Evidence at protein level;
KW Glycoprotein; Golgi apparatus; Membrane; Reference proteome; Signal-anchor;
KW Transferase; Transmembrane; Transmembrane helix; Vacuole.
FT CHAIN 1..491
FT /note="Glucose N-acetyltransferase 1"
FT /id="PRO_0000087536"
FT TOPO_DOM 1..10
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 11..31
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 32..491
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT MOTIF 198..200
FT /note="DXD"
FT CARBOHYD 136
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 177
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 187
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 425
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 491 AA; 57578 MW; 22BD797B1EBF7A32 CRC64;
MRLISKRRIR FIVFILFGVL TVFVVSRLVV HFQYNQEIKF YKKYFQQRKD GLHEIYNPLE
IKQIPKETID DLYTARLDKE LKNGEVIEWS KFAYVNYVTN ADYLCNTLII FNDLKQEFET
KAKLVLLISK DLLDPNTSSN VAYISSLLNK IQAIDEDQVV IKLIDNIVKP KDTTPWNESL
TKLLVFNQTE FDRVIYLDND AILRSSLDEL FFLPNYIKFA APLTYWFLSN SDLEKSYHET
RHREKQPINL QSYTKVLTKR IGKGQMIYNH LPSLPHSLYL NSNNIAQDII SSTSSLSPLF
DFQSSKKVGK LKFASNLMVI NPSKEAFDEI VNVMLPKILN KKEKYDMDLI NEEMYNLKKI
IYKQFIFFRK VRKLFKPEVL VLPFARYGLL TGSLRNPRHY SIIYNDVLGY KTLDNDGNDI
PVGLNDSVAY SKYIHFSDYP LAKPWNYPSM KEFECIVKEE DAEDSKLEHQ ACDLWNSVYA
SYIQSREICL V
