ID   GNTDO_RALSP             Reviewed;         355 AA.
AC   O86041;
DT   06-MAR-2013, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1999, sequence version 2.
DT   03-AUG-2022, entry version 58.
DE   RecName: Full=Gentisate 1,2-dioxygenase {ECO:0000303|PubMed:11133965};
DE            Short=GDO {ECO:0000303|PubMed:11133965};
DE            EC=1.13.11.4 {ECO:0000269|PubMed:11133965};
DE   AltName: Full=Naphthalene degradation protein I;
GN   Name=nagI {ECO:0000312|EMBL:AAD12619.1};
OS   Ralstonia sp.
OG   Plasmid pWWU2 {ECO:0000312|EMBL:AAD12619.1}.
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Ralstonia.
OX   NCBI_TaxID=54061;
RN   [1] {ECO:0000305, ECO:0000312|EMBL:AAD12619.1}
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, SUBSTRATE
RP   SPECIFICITY, BIOPHYSICOCHEMICAL PROPERTIES, AND PATHWAY.
RC   STRAIN=U2 {ECO:0000312|EMBL:AAD12619.1};
RX   PubMed=11133965; DOI=10.1128/jb.183.2.700-708.2001;
RA   Zhou N.Y., Fuenmayor S.L., Williams P.A.;
RT   "nag genes of Ralstonia (formerly Pseudomonas) sp. strain U2 encoding
RT   enzymes for gentisate catabolism.";
RL   J. Bacteriol. 183:700-708(2001).
CC   -!- FUNCTION: Catalyzes the oxygen-dependent ring fission of gentisate
CC       between the carboxyl and proximal hydroxyl groups at positions 1 and 2
CC       of the aromatic ring to form maleylpyruvate. Can also catalyze
CC       oxidation of alkyl- and halogenated gentisates. Exhibits higher
CC       affinity for 3-substituted gentisates than for gentisate but has higher
CC       activity with gentisate. {ECO:0000269|PubMed:11133965}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2,5-dihydroxybenzoate + O2 = 3-maleylpyruvate + H(+);
CC         Xref=Rhea:RHEA:18237, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:16727, ChEBI:CHEBI:58044; EC=1.13.11.4;
CC         Evidence={ECO:0000269|PubMed:11133965};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=22.4 uM for gentisate {ECO:0000269|PubMed:11133965};
CC         KM=10.7 uM for 3-methylgentisate {ECO:0000269|PubMed:11133965};
CC         KM=5.3 uM for 3-bromogentisate {ECO:0000269|PubMed:11133965};
CC   -!- PATHWAY: Aromatic compound metabolism; naphthalene degradation.
CC       {ECO:0000269|PubMed:11133965}.
CC   -!- SIMILARITY: Belongs to the gentisate 1,2-dioxygenase family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AF036940; AAD12619.1; -; Genomic_DNA.
DR   AlphaFoldDB; O86041; -.
DR   SMR; O86041; -.
DR   PRIDE; O86041; -.
DR   BioCyc; MetaCyc:MON-14769; -.
DR   SABIO-RK; O86041; -.
DR   UniPathway; UPA00082; -.
DR   GO; GO:0047922; F:gentisate 1,2-dioxygenase activity; IDA:UniProtKB.
DR   GO; GO:1901170; P:naphthalene catabolic process; IDA:UniProtKB.
DR   Gene3D; 2.60.120.10; -; 1.
DR   InterPro; IPR013096; Cupin_2.
DR   InterPro; IPR014710; RmlC-like_jellyroll.
DR   InterPro; IPR011051; RmlC_Cupin_sf.
DR   Pfam; PF07883; Cupin_2; 1.
DR   SUPFAM; SSF51182; SSF51182; 1.
PE   1: Evidence at protein level;
KW   Aromatic hydrocarbons catabolism; Dioxygenase; Oxidoreductase; Plasmid.
FT   CHAIN           1..355
FT                   /note="Gentisate 1,2-dioxygenase"
FT                   /id="PRO_0000421467"
SQ   SEQUENCE   355 AA;  39886 MW;  F16D986FCBA08FB2 CRC64;
     MLDEEERITM SHELGRLEDL PQDYRDELKQ LNLVPLWPSL RAVLPPNVPT RQTQPTYWSY
     QTLKPLLLKA GELTPIEKAE RRVLVLANPG HGLEKMQASA AIYLGMQLLL PGEWAPSHRH
     TPNAVRMIVE GEGAYTTVDG EKCPMSRGDL ILTPTGLWHE HGHDGNEPVV WLDVLDLPLV
     YYMEASYHID GERQQVDPGR GDCAWTRAGV VPTPVFQRSD KRYPLLRYPW ADTRAALLSL
     AADQPEQECV QVTYVNPETG DDAENILGFY ALMLKPGQTL RLPVRSPAVV FHQIEGRSEA
     RIAESTFALR EADTCCAPGY TEVTLKNLSA DQPSFIFMAD ESPLHRKLGV FENRG