AMPA_ANAPZ
ID AMPA_ANAPZ Reviewed; 578 AA.
AC Q2GIB5;
DT 07-SEP-2016, integrated into UniProtKB/Swiss-Prot.
DT 21-MAR-2006, sequence version 1.
DT 03-AUG-2022, entry version 58.
DE RecName: Full=SUMOylated effector protein AmpA {ECO:0000303|PubMed:25308709};
DE AltName: Full=P100 {ECO:0000303|PubMed:20212090};
GN Name=ampA {ECO:0000303|PubMed:25308709};
GN OrderedLocusNames=APH_1387 {ECO:0000312|EMBL:ABD44281.1};
OS Anaplasma phagocytophilum (strain HZ).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales;
OC Anaplasmataceae; Anaplasma; phagocytophilum group.
OX NCBI_TaxID=212042;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=HZ;
RX PubMed=16482227; DOI=10.1371/journal.pgen.0020021;
RA Dunning Hotopp J.C., Lin M., Madupu R., Crabtree J., Angiuoli S.V.,
RA Eisen J.A., Seshadri R., Ren Q., Wu M., Utterback T.R., Smith S., Lewis M.,
RA Khouri H., Zhang C., Niu H., Lin Q., Ohashi N., Zhi N., Nelson W.C.,
RA Brinkac L.M., Dodson R.J., Rosovitz M.J., Sundaram J.P., Daugherty S.C.,
RA Davidsen T., Durkin A.S., Gwinn M.L., Haft D.H., Selengut J.D.,
RA Sullivan S.A., Zafar N., Zhou L., Benahmed F., Forberger H., Halpin R.,
RA Mulligan S., Robinson J., White O., Rikihisa Y., Tettelin H.;
RT "Comparative genomics of emerging human ehrlichiosis agents.";
RL PLoS Genet. 2:208-222(2006).
RN [2]
RP SUBCELLULAR LOCATION, INDUCTION, AND REPEAT.
RC STRAIN=HZ;
RX PubMed=20212090; DOI=10.1128/iai.01418-09;
RA Huang B., Troese M.J., Ye S., Sims J.T., Galloway N.L., Borjesson D.L.,
RA Carlyon J.A.;
RT "Anaplasma phagocytophilum APH_1387 is expressed throughout bacterial
RT intracellular development and localizes to the pathogen-occupied vacuolar
RT membrane.";
RL Infect. Immun. 78:1864-1873(2010).
RN [3]
RP FUNCTION, SUMOYLATION, AND SUBCELLULAR LOCATION.
RX PubMed=25308709; DOI=10.1111/cmi.12380;
RA Beyer A.R., Truchan H.K., May L.J., Walker N.J., Borjesson D.L.,
RA Carlyon J.A.;
RT "The Anaplasma phagocytophilum effector AmpA hijacks host cell
RT SUMOylation.";
RL Cell. Microbiol. 17:504-519(2015).
CC -!- FUNCTION: Secreted effector that hijacks host cell SUMOylation during
CC A.phagocytophilum infection and is important for the pathogen's
CC intracellular survival. {ECO:0000269|PubMed:25308709}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:20212090,
CC ECO:0000269|PubMed:25308709}. Host membrane
CC {ECO:0000269|PubMed:20212090}; Multi-pass membrane protein
CC {ECO:0000305|PubMed:20212090}. Host cytoplasm, host cytosol
CC {ECO:0000269|PubMed:25308709}. Note=Localizes to the A.phagocytophilum-
CC occupied vacuolar membrane (AVM) (PubMed:20212090). Potentially
CC traverses the AVM 3 or fewer times by means of the 3 bilobed
CC hydrophobic alpha-helical domains that are predicted to be in each
CC direct repeat (PubMed:20212090). Colocalizes with SUMO2/3 on the AVM
CC and in the host cytosol throughout the infection cycle
CC (PubMed:25308709). Only late during the infection cycle AmpA
CC colocalizes with SUMO1, which terminally caps poly-SUMO2/3 chains
CC (PubMed:25308709). Is probably not secreted via the type IV secretion
CC system (T4SS) (PubMed:20212090). {ECO:0000269|PubMed:20212090,
CC ECO:0000269|PubMed:25308709}.
CC -!- INDUCTION: Is induced within the initial hours of A.phagocytophilum
CC infection and is strongly expressed during the bacterium's
CC intracellular replication phase. {ECO:0000269|PubMed:20212090}.
CC -!- PTM: Polysumoylated during infection on at least two lysine residues,
CC in the N- and C-terminal section. SUMO2/3 modification of AmpA
CC throughout the infection cycle is likely critical for bacterial
CC intracellular survival, while terminal SUMO1 conjugation of AmpA may
CC promote a late-stage infection cycle event. Only a small portion of the
CC available AmpA pool is actually SUMOylated at any given time.
CC {ECO:0000269|PubMed:25308709}.
CC -!- MISCELLANEOUS: Pharmacologically inhibiting sumoylation in infected
CC cells significantly reduces the bacterial load.
CC {ECO:0000269|PubMed:25308709}.
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DR EMBL; CP000235; ABD44281.1; -; Genomic_DNA.
DR RefSeq; WP_011451392.1; NC_007797.1.
DR AlphaFoldDB; Q2GIB5; -.
DR STRING; 212042.APH_1387; -.
DR EnsemblBacteria; ABD44281; ABD44281; APH_1387.
DR GeneID; 56369209; -.
DR KEGG; aph:APH_1387; -.
DR HOGENOM; CLU_471483_0_0_5; -.
DR OrthoDB; 2228900at2; -.
DR Proteomes; UP000001943; Chromosome.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0044162; C:host cell cytoplasmic vesicle membrane; IDA:UniProtKB.
DR GO; GO:0044164; C:host cell cytosol; IDA:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW Host cytoplasm; Host membrane; Membrane; Reference proteome; Repeat;
KW Secreted; Transmembrane; Transmembrane helix; Ubl conjugation.
FT CHAIN 1..578
FT /note="SUMOylated effector protein AmpA"
FT /id="PRO_0000436967"
FT REPEAT 180..272
FT /note="1"
FT /evidence="ECO:0000305|PubMed:20212090"
FT REPEAT 304..425
FT /note="2"
FT /evidence="ECO:0000305|PubMed:20212090"
FT REPEAT 428..557
FT /note="3"
FT /evidence="ECO:0000305|PubMed:20212090"
FT REGION 144..169
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 180..557
FT /note="3 X approximate tandem repeats"
FT /evidence="ECO:0000305|PubMed:20212090"
FT REGION 516..578
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 149..163
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 578 AA; 61403 MW; 8E365826661C3BCE CRC64;
MYGIDIELSD YRIGSETISS GDDGYYEGCA CDKDASTNAY SYDKCRVVRG TWRPSELVLY
VGDEHVACRD VASGMHHGNL PGKVYFIEAE AGRAATAEGG VYTTVVEALS LVQEEEGTGM
YLINAPEKAV VRFFKIEKSA AEEPQTVDPS VVESATGSGV DTQEEQEIDQ EAPAIEEVET
EEQEVILEEG TLIDLEQPVA QVPVVAEAEL PGVEAAEAIV PSLEENKLQE VVVAPEAQQL
ESAPEVSAPA QPESTVLGVA EGDLKSEVSV EANADVAQKE VISGQQEQEI AEALEGTEAP
VEVKEETEVL LKEDTLIDLE QPVAQVPVVA EAELPGVEAA EAIVPSLEEN KLQEVVVAPE
AQQLESAPEV SAPAQPESTV LGVTEGDLKS EVSVEADAGM QQEAGISDQE TQATEEVEKV
EVSVETKTEE PEVILEEGTL IDLEQPVAQV PVVAEAELPG VEAAEAIVPS LEENKLQEVV
VAPEAQQLES APEVSAPVQP ESTVLGVTEG DLKSEVSVEA DAGMQQEAGI SDQETQATEE
VEKVEVSVEA DAGMQQELVD VPTALPLKDP DDEDVLSY
