GNTR_BACSU
ID GNTR_BACSU Reviewed; 243 AA.
AC P10585;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1989, sequence version 2.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=Gluconate operon transcriptional repressor;
DE AltName: Full=P28 protein;
GN Name=gntR; OrderedLocusNames=BSU40050;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3020045; DOI=10.1016/s0021-9258(18)67083-8;
RA Fujita Y., Fujita T., Miwa Y., Nihashi J., Aratani Y.;
RT "Organization and transcription of the gluconate operon, gnt, of Bacillus
RT subtilis.";
RL J. Biol. Chem. 261:13744-13753(1986).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / BGSC1A1;
RA Fujita Y.;
RL Submitted (MAY-1994) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / BGSC1A1;
RX PubMed=7584049; DOI=10.1093/dnares/2.2.61;
RA Yoshida K., Seki S., Fujimura M., Miwa Y., Fujita Y.;
RT "Cloning and sequencing of a 36-kb region of the Bacillus subtilis genome
RT between the gnt and iol operons.";
RL DNA Res. 2:61-69(1995).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-169.
RX PubMed=2419835; DOI=10.1093/nar/14.3.1237;
RA Fujita Y., Fujita T.;
RT "Identification and nucleotide sequence of the promoter region of the
RT Bacillus subtilis gluconate operon.";
RL Nucleic Acids Res. 14:1237-1252(1986).
RN [6]
RP MUTAGENESIS.
RX PubMed=8510140; DOI=10.1006/jmbi.1993.1270;
RA Yoshida K., Fujita Y., Sarai A.;
RT "Missense mutations in the Bacillus subtilis gnt repressor that diminish
RT operator binding ability.";
RL J. Mol. Biol. 231:167-174(1993).
CC -!- FUNCTION: Transcriptional repressor of the gluconate operon (gntRKPZ),
CC which encodes the proteins for gluconate utilization. Represses mRNA
CC synthesis by binding to the gnt operator; the binding is suppressed by
CC gluconate or glucono-delta-lactone.
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DR EMBL; J02584; AAA56924.1; -; Genomic_DNA.
DR EMBL; AB005554; BAA21579.1; -; Genomic_DNA.
DR EMBL; X03510; CAA27222.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB16042.1; -; Genomic_DNA.
DR PIR; C26190; C26190.
DR RefSeq; NP_391885.1; NC_000964.3.
DR RefSeq; WP_003243132.1; NZ_JNCM01000034.1.
DR AlphaFoldDB; P10585; -.
DR SMR; P10585; -.
DR STRING; 224308.BSU40050; -.
DR PaxDb; P10585; -.
DR PRIDE; P10585; -.
DR EnsemblBacteria; CAB16042; CAB16042; BSU_40050.
DR GeneID; 937710; -.
DR KEGG; bsu:BSU40050; -.
DR PATRIC; fig|224308.179.peg.4332; -.
DR eggNOG; COG1802; Bacteria.
DR InParanoid; P10585; -.
DR OMA; DNHELYI; -.
DR PhylomeDB; P10585; -.
DR BioCyc; BSUB:BSU40050-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0019521; P:D-gluconate metabolic process; IEA:UniProtKB-KW.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 1.20.120.530; -; 1.
DR InterPro; IPR011711; GntR_C.
DR InterPro; IPR008920; TF_FadR/GntR_C.
DR InterPro; IPR000524; Tscrpt_reg_HTH_GntR.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR Pfam; PF07729; FCD; 1.
DR Pfam; PF00392; GntR; 1.
DR PRINTS; PR00035; HTHGNTR.
DR SMART; SM00895; FCD; 1.
DR SMART; SM00345; HTH_GNTR; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR SUPFAM; SSF48008; SSF48008; 1.
DR PROSITE; PS50949; HTH_GNTR; 1.
PE 4: Predicted;
KW DNA-binding; Gluconate utilization; Reference proteome; Repressor;
KW Transcription; Transcription regulation.
FT CHAIN 1..243
FT /note="Gluconate operon transcriptional repressor"
FT /id="PRO_0000050648"
FT DOMAIN 17..83
FT /note="HTH gntR-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00307"
FT DNA_BIND 44..63
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00307"
SQ SEQUENCE 243 AA; 28279 MW; 9757EC5B36EF2BB1 CRC64;
MLDSKDLLYP AKWLSKASTG VRVAYELRMR IVSGLIESGT ILSENTIAAE FSVSRSPVRE
ALKILASEKI IRLERMGAVV IGLTEKKIAE IYDVRLLLET FVFERLVKID IEPLVKDLSK
ILEMMKVSIK YEDADEFSFQ DVLFHETIIR AIDHSYIQMI WNNLKPVMES FILLSMRVRL
KEKYEDFTRI LDNHELYIQA IKTKDRALMI QSLHQNFDDV QDKVEDLWLS QQMLAKGAEY
NND
