GN_HCMVA
ID GN_HCMVA Reviewed; 138 AA.
AC P16795; Q7M6L9;
DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1990, sequence version 1.
DT 03-AUG-2022, entry version 78.
DE RecName: Full=Envelope glycoprotein N {ECO:0000255|HAMAP-Rule:MF_04037};
DE Flags: Precursor;
GN Name=gN {ECO:0000255|HAMAP-Rule:MF_04037}; ORFNames=UL73;
OS Human cytomegalovirus (strain AD169) (HHV-5) (Human herpesvirus 5).
OC Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC Herpesvirales; Herpesviridae; Betaherpesvirinae; Cytomegalovirus.
OX NCBI_TaxID=10360;
OH NCBI_TaxID=9606; Homo sapiens (Human).
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=2161319; DOI=10.1007/978-3-642-74980-3_6;
RA Chee M.S., Bankier A.T., Beck S., Bohni R., Brown C.M., Cerny R.,
RA Horsnell T., Hutchison C.A. III, Kouzarides T., Martignetti J.A.,
RA Preddie E., Satchwell S.C., Tomlinson P., Weston K.M., Barrell B.G.;
RT "Analysis of the protein-coding content of the sequence of human
RT cytomegalovirus strain AD169.";
RL Curr. Top. Microbiol. Immunol. 154:125-169(1990).
RN [2]
RP GENOME REANNOTATION.
RX PubMed=12533697; DOI=10.1099/vir.0.18606-0;
RA Davison A.J., Dolan A., Akter P., Addison C., Dargan D.J., Alcendor D.J.,
RA McGeoch D.J., Hayward G.S.;
RT "The human cytomegalovirus genome revisited: comparison with the chimpanzee
RT cytomegalovirus genome.";
RL J. Gen. Virol. 84:17-28(2003).
RN [3]
RP ERRATUM OF PUBMED:12533697.
RA Davison A.J., Dolan A., Akter P., Addison C., Dargan D.J., Alcendor D.J.,
RA McGeoch D.J., Hayward G.S.;
RL J. Gen. Virol. 84:1053-1053(2003).
RN [4]
RP IDENTIFICATION.
RX PubMed=15452216; DOI=10.1128/jvi.78.20.10960-10966.2004;
RA Varnum S.M., Streblow D.N., Monroe M.E., Smith P., Auberry K.J.,
RA Pasa-Tolic L., Wang D., Camp D.G. II, Rodland K., Wiley S., Britt W.,
RA Shenk T., Smith R.D., Nelson J.A.;
RT "Identification of proteins in human cytomegalovirus (HCMV) particles: the
RT HCMV proteome.";
RL J. Virol. 78:10960-10966(2004).
RN [5]
RP ERRATUM OF PUBMED:15452216.
RA Varnum S.M., Streblow D.N., Monroe M.E., Smith P., Auberry K.J.,
RA Pasa-Tolic L., Wang D., Camp D.G. II, Rodland K., Wiley S., Britt W.,
RA Shenk T., Smith R.D., Nelson J.A.;
RL J. Virol. 78:13395-13395(2004).
RN [6]
RP SUBUNIT, SUBCELLULAR LOCATION, GLYCOSYLATION, AND DISULFIDE BOND.
RX PubMed=11090188; DOI=10.1128/jvi.74.24.11881-11892.2000;
RA Mach M., Kropff B., Dal Monte P., Britt W.;
RT "Complex formation by human cytomegalovirus glycoproteins M (gpUL100) and N
RT (gpUL73).";
RL J. Virol. 74:11881-11892(2000).
RN [7]
RP SUBCELLULAR LOCATION.
RX PubMed=12111433; DOI=10.1007/s00705-002-0810-x;
RA Pignatelli S., Dal Monte P., Zini N., Valmori A., Maraldi N.M.,
RA Landini M.P.;
RT "Immunoelectron microscopy analysis of HCMV gpUL73 (gN) localization.";
RL Arch. Virol. 147:1247-1256(2002).
RN [8]
RP SUBUNIT, AND DISULFIDE BOND.
RX PubMed=15681419; DOI=10.1128/jvi.79.4.2160-2170.2005;
RA Mach M., Kropff B., Kryzaniak M., Britt W.;
RT "Complex formation by glycoproteins M and N of human cytomegalovirus:
RT structural and functional aspects.";
RL J. Virol. 79:2160-2170(2005).
RN [9]
RP FUNCTION.
RX PubMed=17229708; DOI=10.1128/jvi.01463-06;
RA Mach M., Osinski K., Kropff B., Schloetzer-Schrehardt U., Krzyzaniak M.,
RA Britt W.;
RT "The carboxy-terminal domain of glycoprotein N of human cytomegalovirus is
RT required for virion morphogenesis.";
RL J. Virol. 81:5212-5224(2007).
CC -!- FUNCTION: Envelope glycoprotein necessary for proper maturation of gM
CC and modulation of its membrane fusion activity. Also plays a critical
CC role in virion morphogenesis. {ECO:0000255|HAMAP-Rule:MF_04037,
CC ECO:0000269|PubMed:17229708}.
CC -!- SUBUNIT: Interacts (via N-terminus) with gM (via N-terminus). The gM-gN
CC heterodimer forms the gCII complex. {ECO:0000255|HAMAP-Rule:MF_04037,
CC ECO:0000269|PubMed:11090188, ECO:0000269|PubMed:15681419}.
CC -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000255|HAMAP-Rule:MF_04037,
CC ECO:0000269|PubMed:12111433}; Single-pass type I membrane protein
CC {ECO:0000255|HAMAP-Rule:MF_04037}. Host membrane {ECO:0000255|HAMAP-
CC Rule:MF_04037, ECO:0000269|PubMed:12111433}; Single-pass type I
CC membrane protein {ECO:0000255|HAMAP-Rule:MF_04037}. Host Golgi
CC apparatus, host trans-Golgi network {ECO:0000255|HAMAP-Rule:MF_04037}.
CC Note=When coexpressed with gM, localizes in the host trans-Golgi
CC network. {ECO:0000255|HAMAP-Rule:MF_04037}.
CC -!- PTM: O-glycosylated. {ECO:0000269|PubMed:11090188}.
CC -!- SIMILARITY: Belongs to the herpesviridae glycoprotein N family.
CC {ECO:0000255|HAMAP-Rule:MF_04037}.
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DR EMBL; X17403; CAA35388.1; -; Genomic_DNA.
DR EMBL; BK000394; DAA00169.1; -; Genomic_DNA.
DR PIR; S09836; QQBEB2.
DR Proteomes; UP000008991; Genome.
DR Proteomes; UP000008992; Genome.
DR GO; GO:0044177; C:host cell Golgi apparatus; IEA:UniProtKB-SubCell.
DR GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0019031; C:viral envelope; IEA:UniProtKB-KW.
DR GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR HAMAP; MF_04037; HSV_GN; 1.
DR InterPro; IPR021003; Cytomegalovirus_glycopN_N.
DR InterPro; IPR005211; Herpes_glycoprotein_N_domain.
DR InterPro; IPR034707; HSV_GN.
DR Pfam; PF03554; Herpes_UL73; 1.
DR Pfam; PF12522; UL73_N; 1.
PE 1: Evidence at protein level;
KW Disulfide bond; Host Golgi apparatus; Host membrane; Membrane;
KW Reference proteome; Signal; Transmembrane; Transmembrane helix;
KW Viral envelope protein; Virion.
FT SIGNAL 1..21
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04037"
FT CHAIN 22..138
FT /note="Envelope glycoprotein N"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04037"
FT /id="PRO_0000116220"
FT TOPO_DOM 22..101
FT /note="Virion surface"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04037"
FT TRANSMEM 102..122
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04037"
FT TOPO_DOM 123..138
FT /note="Intravirion"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04037"
FT DISULFID 90
FT /note="Interchain (with gM)"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04037,
FT ECO:0000269|PubMed:11090188, ECO:0000269|PubMed:15681419"
SQ SEQUENCE 138 AA; 14868 MW; 5AEB2A7CA0F4CA09 CRC64;
MEWNTLVLGL LVLSVVAESS GNNSSTSTSA TTSKSSASVS TTKLTTVATT SATTTTTTTL
STTSTKLSST THDPNVMRRH ANDDFYKAHC TSHMYELSLS SFAAWWTMLN ALILMGAFCI
VLRHCCFQNF TATTTKGY
