GO45_RAT
ID GO45_RAT Reviewed; 400 AA.
AC Q6AYB8;
DT 10-OCT-2018, integrated into UniProtKB/Swiss-Prot.
DT 13-SEP-2004, sequence version 1.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=Golgin-45;
DE AltName: Full=Basic leucine zipper nuclear factor 1 {ECO:0000312|Ensembl:ENSRNOP00000046014};
GN Name=Blzf1 {ECO:0000312|Ensembl:ENSRNOP00000046014,
GN ECO:0000312|RGD:1562452};
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116 {ECO:0000312|EMBL:AAH79114.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Brown Norway;
RX PubMed=15057822; DOI=10.1038/nature02426;
RA Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J.,
RA Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G.,
RA Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G.,
RA Morgan M., Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G.,
RA Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S.,
RA Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T.,
RA Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D.,
RA Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L.,
RA Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D.,
RA Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M.,
RA Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C.,
RA Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J.,
RA Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H.,
RA Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X.,
RA Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q.,
RA Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P.,
RA Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A.,
RA Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C.,
RA Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J.,
RA Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J.,
RA Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F.,
RA Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A.,
RA Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A.,
RA Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J.,
RA Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E.,
RA Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M.,
RA Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C.,
RA Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L.,
RA Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W.,
RA Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y.,
RA Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V.,
RA Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M.,
RA Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S.,
RA Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B.,
RA Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R.,
RA Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J.,
RA Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D.,
RA Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S.,
RA Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S.,
RA Mockrin S., Collins F.S.;
RT "Genome sequence of the Brown Norway rat yields insights into mammalian
RT evolution.";
RL Nature 428:493-521(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Kidney {ECO:0000312|EMBL:AAH79114.1};
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP INTERACTION WITH GORASP2 AND RAB2, SUBCELLULAR LOCATION, AND IDENTIFICATION
RP BY MASS SPECTROMETRY.
RX PubMed=11739401; DOI=10.1083/jcb.200108079;
RA Short B., Preisinger C., Koerner R., Kopajtich R., Byron O., Barr F.A.;
RT "A GRASP55-rab2 effector complex linking Golgi structure to membrane
RT traffic.";
RL J. Cell Biol. 155:877-883(2001).
CC -!- FUNCTION: Required for normal Golgi structure and for protein transport
CC from the endoplasmic reticulum (ER) through the Golgi apparatus to the
CC cell surface. {ECO:0000250|UniProtKB:Q9H2G9}.
CC -!- SUBUNIT: Interacts with GORASP2 (PubMed:11739401). Interacts with the
CC GTP-bound form of RAB2, but not with other Golgi Rab proteins
CC (PubMed:11739401). Identified in a complex with RAB2 and GORASP2
CC (PubMed:11739401). {ECO:0000269|PubMed:11739401}.
CC -!- INTERACTION:
CC Q6AYB8; Q9R064: Gorasp2; NbExp=5; IntAct=EBI-4422894, EBI-4422912;
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000269|PubMed:11739401}.
CC -!- DOMAIN: The tankyrase-binding motif (also named TBD) is required for
CC interaction with tankyrase TNKS and TNKS2.
CC {ECO:0000250|UniProtKB:Q9H2G9}.
CC -!- PTM: ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated
CC protein is recognized by RNF146, followed by ubiquitination.
CC {ECO:0000250|UniProtKB:Q9H2G9}.
CC -!- PTM: Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its
CC degradation. {ECO:0000250|UniProtKB:Q9H2G9}.
CC -!- CAUTION: Was initially thought to be a potential transcription factor,
CC localized in the nucleus. {ECO:0000305}.
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DR EMBL; AABR07021628; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH473958; EDM09357.1; -; Genomic_DNA.
DR EMBL; BC079114; AAH79114.1; -; mRNA.
DR RefSeq; NP_001017494.1; NM_001017494.1.
DR AlphaFoldDB; Q6AYB8; -.
DR SMR; Q6AYB8; -.
DR CORUM; Q6AYB8; -.
DR IntAct; Q6AYB8; 2.
DR STRING; 10116.ENSRNOP00000046014; -.
DR PaxDb; Q6AYB8; -.
DR Ensembl; ENSRNOT00000051763; ENSRNOP00000046014; ENSRNOG00000002884.
DR GeneID; 498266; -.
DR KEGG; rno:498266; -.
DR UCSC; RGD:1562452; rat.
DR CTD; 8548; -.
DR RGD; 1562452; Blzf1.
DR eggNOG; KOG4074; Eukaryota.
DR GeneTree; ENSGT00390000009400; -.
DR HOGENOM; CLU_057527_0_0_1; -.
DR InParanoid; Q6AYB8; -.
DR OMA; HVVPTKN; -.
DR OrthoDB; 1218216at2759; -.
DR PhylomeDB; Q6AYB8; -.
DR Reactome; R-RNO-162658; Golgi Cisternae Pericentriolar Stack Reorganization.
DR PRO; PR:Q6AYB8; -.
DR Proteomes; UP000002494; Chromosome 13.
DR Proteomes; UP000234681; Chromosome 13.
DR Bgee; ENSRNOG00000002884; Expressed in esophagus and 19 other tissues.
DR GO; GO:0005794; C:Golgi apparatus; ISO:RGD.
DR GO; GO:0000139; C:Golgi membrane; IDA:RGD.
DR GO; GO:0005634; C:nucleus; ISO:RGD.
DR GO; GO:0019899; F:enzyme binding; ISO:RGD.
DR GO; GO:0031625; F:ubiquitin protein ligase binding; ISO:RGD.
DR GO; GO:0007030; P:Golgi organization; ISO:RGD.
DR GO; GO:0043001; P:Golgi to plasma membrane protein transport; ISO:RGD.
DR InterPro; IPR027095; Golgin-45.
DR InterPro; IPR013183; Hsk3-like.
DR PANTHER; PTHR13066; PTHR13066; 1.
DR Pfam; PF08227; DASH_Hsk3; 1.
PE 1: Evidence at protein level;
KW ADP-ribosylation; Coiled coil; ER-Golgi transport; Golgi apparatus;
KW Membrane; Phosphoprotein; Protein transport; Reference proteome; Transport;
KW Ubl conjugation.
FT CHAIN 1..400
FT /note="Golgin-45"
FT /id="PRO_0000445242"
FT REGION 1..36
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 394..400
FT /note="Essential for interaction with GORASP2"
FT /evidence="ECO:0000250|UniProtKB:Q9H2G9"
FT COILED 126..263
FT /evidence="ECO:0000255"
FT MOTIF 22..26
FT /note="Tankyrase-binding motif"
FT /evidence="ECO:0000250|UniProtKB:Q9H2G9"
FT MOD_RES 53
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9H2G9"
FT MOD_RES 356
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9H2G9"
SQ SEQUENCE 400 AA; 45089 MW; F5AABB4D0D52E307 CRC64;
MEKMTTLKSF ESKGILTSTP IRGAGDGMET EEPPKSVEVT HGVQPINQHV LPSPRKKVPS
DSPGVLQLGK ILNEKTVEVE AVRIFVPKAA ITHDIPTKSA KVKSLGHHRE ELHNQSEVVT
DPRKELSEVK KVLEKLKNSE RRLLQDKEGL SNQLRVQTEV NRELKKLLVA SVGDDPQYHF
ERLAREKNQL ILENEALGRN TAQLSEQLER MSIQCDVWRS KFLASRVMAD ELTNFRVVLQ
HQNRDAQSAI QDLLSEREQF RQEMISTQKF LEELLVSLQW GREQTYSPNT QPHSTADLAL
TNHRLAEAVH AHLLGNVGIS HPKRTPATAE VCSTPAEKMA EKVLRILDPV ACTESSPDNA
FAESTLLTTK KNIGRFHPYT RYENITFNCC SHCQGELLAL
