GOE_DROME
ID GOE_DROME Reviewed; 879 AA.
AC Q9XZ14;
DT 07-SEP-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Protein gone early {ECO:0000312|FlyBase:FBgn0027528};
GN Name=goe {ECO:0000312|FlyBase:FBgn0027528};
GN ORFNames=CG9634 {ECO:0000312|FlyBase:FBgn0027528};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:AAD34757.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAD34757.1};
RC TISSUE=Embryo {ECO:0000312|EMBL:AAD34757.1};
RX PubMed=10731138; DOI=10.1126/science.287.5461.2222;
RA Rubin G.M., Hong L., Brokstein P., Evans-Holm M., Frise E., Stapleton M.,
RA Harvey D.A.;
RT "A Drosophila complementary DNA resource.";
RL Science 287:2222-2224(2000).
RN [4] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP AND DISRUPTION PHENOTYPE.
RX PubMed=25420147; DOI=10.1371/journal.pone.0113423;
RA Matsuoka S., Gupta S., Suzuki E., Hiromi Y., Asaoka M.;
RT "gone early, a novel germline factor, ensures the proper size of the stem
RT cell precursor pool in the Drosophila ovary.";
RL PLoS ONE 9:E113423-E113423(2014).
CC -!- FUNCTION: Plays a role in the ovary in limiting the number of
CC primordial germ cells (PGCs) that develop directly into gametes,
CC allowing them instead to enter the developmental pathway that produces
CC germline stem cells (GSCs) from PGCs and ensuring lifelong production
CC of gametes from these GSCs. Negatively regulates epidermal growth
CC factor receptor (Egfr) signaling. Probably down-regulates EGFR
CC signaling on intermingled cells, a type of somatic stromal cell which
CC contacts PGCs, and the resultant low level of signaling limits the
CC proportion of PGCs which start gametogenesis, maintaining them in an
CC undifferentiated proliferative state. {ECO:0000269|PubMed:25420147}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:25420147};
CC Single-pass type II membrane protein {ECO:0000255}. Note=Not uniformly
CC present at the cell membrane but is concentrated at the interface
CC between germ cells. {ECO:0000269|PubMed:25420147}.
CC -!- TISSUE SPECIFICITY: In late third larval instar ovaries, detected in
CC germ cells but not in somatic intermingled cells and is expressed
CC uniformly in the wing disk. In the embryo, expressed in the ventral
CC midline, tracheal placodes, and cells forming the anterior-most row in
CC each segment. {ECO:0000269|PubMed:25420147}.
CC -!- DEVELOPMENTAL STAGE: In the larva, becomes detectable at the late
CC second larval instar stage and continues to be expressed throughout the
CC larval period. {ECO:0000269|PubMed:25420147}.
CC -!- DISRUPTION PHENOTYPE: Increase in the number of differentiating germ
CC cells. {ECO:0000269|PubMed:25420147}.
CC -!- SIMILARITY: Belongs to the peptidase M13 family. {ECO:0000255|PROSITE-
CC ProRule:PRU01233, ECO:0000305}.
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DR EMBL; AE014298; AAF48659.1; -; Genomic_DNA.
DR EMBL; AE014298; AHN59820.1; -; Genomic_DNA.
DR EMBL; AF132169; AAD34757.1; -; mRNA.
DR RefSeq; NP_001285350.1; NM_001298421.1.
DR RefSeq; NP_573160.1; NM_132932.3.
DR AlphaFoldDB; Q9XZ14; -.
DR SMR; Q9XZ14; -.
DR IntAct; Q9XZ14; 1.
DR STRING; 7227.FBpp0074133; -.
DR GlyGen; Q9XZ14; 4 sites.
DR PaxDb; Q9XZ14; -.
DR EnsemblMetazoa; FBtr0074359; FBpp0074133; FBgn0027528.
DR EnsemblMetazoa; FBtr0343364; FBpp0310021; FBgn0027528.
DR GeneID; 32660; -.
DR KEGG; dme:Dmel_CG9634; -.
DR UCSC; CG9634-RA; d. melanogaster.
DR CTD; 32660; -.
DR FlyBase; FBgn0027528; goe.
DR VEuPathDB; VectorBase:FBgn0027528; -.
DR eggNOG; KOG3624; Eukaryota.
DR GeneTree; ENSGT00940000164877; -.
DR InParanoid; Q9XZ14; -.
DR OrthoDB; 198642at2759; -.
DR PhylomeDB; Q9XZ14; -.
DR Reactome; R-DME-2022377; Metabolism of Angiotensinogen to Angiotensins.
DR Reactome; R-DME-6798695; Neutrophil degranulation.
DR BioGRID-ORCS; 32660; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 32660; -.
DR PRO; PR:Q9XZ14; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0027528; Expressed in capitellum (Drosophila) and 33 other tissues.
DR ExpressionAtlas; Q9XZ14; baseline and differential.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:FlyBase.
DR GO; GO:2000737; P:negative regulation of stem cell differentiation; IMP:FlyBase.
DR GO; GO:0048477; P:oogenesis; IMP:FlyBase.
DR GO; GO:0016485; P:protein processing; IBA:GO_Central.
DR Gene3D; 1.10.1380.10; -; 1.
DR Gene3D; 3.40.390.10; -; 1.
DR InterPro; IPR024079; MetalloPept_cat_dom_sf.
DR InterPro; IPR000718; Peptidase_M13.
DR InterPro; IPR042089; Peptidase_M13_dom_2.
DR InterPro; IPR008753; Peptidase_M13_N.
DR PANTHER; PTHR11733; PTHR11733; 1.
DR Pfam; PF05649; Peptidase_M13_N; 1.
DR PROSITE; PS51885; NEPRILYSIN; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Differentiation; Disulfide bond; Glycoprotein; Membrane;
KW Oogenesis; Reference proteome; Signal-anchor; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..879
FT /note="Protein gone early"
FT /evidence="ECO:0000305"
FT /id="PRO_0000437210"
FT TOPO_DOM 1..127
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 128..148
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 149..879
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT DOMAIN 162..878
FT /note="Peptidase M13"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01233"
FT CARBOHYD 180
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 539
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 701
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 853
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT DISULFID 163..168
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01233"
FT DISULFID 186..868
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01233"
FT DISULFID 194..826
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01233"
FT DISULFID 254..504
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01233"
FT DISULFID 732..875
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01233"
SQ SEQUENCE 879 AA; 100068 MW; 5D06C9000BF5BB48 CRC64;
MAINQGKYKP SICAIDEVDD RSFKLHNSQD EIGGKQQLQQ IEGSETAPLT TNQQKLHMDD
GSHLTPKDLE AARQDALNEV AAGGAGGRTP AAITPNSTHP SANGLRALLL PGRRSFDALM
SLSRKRRILY VTTACLCALL LLIIILLLAF WPEVPFYLRA PLCLDKECVE SSRQLLLWAN
TSKSPCHETY EWACGNFASD YANHEYFVIK RGEWNYETYN EYQELNELNR FIAMLPNSQE
GSVESTVSSL YRSCRETDVL DKSQSDLLLK RAINAVYGWQ AFRDSNRLQS WEYKRVLVVL
QAKHGIFPYY RVTVENGFSN PHDYVITLDE GELGLPDKYF YGNDADEEVV RGYKLLLRDF
AINMGIVSRE ADLFADDIFH YERRIVNHIE DAKADANRPI NKLMRLADLK IKAPSLPILE
SLQAIFPKTK ITEDTEVLVR DPEVMHALSV LLSTSDKKPI NNFIVWSLAR KMLPHLSKEY
RTLAETFDHA LYGRTASYPR WLICSKVVRD WLPFAVDALQ QQPPRQQFET MTSKSRGLNK
TQGNEELLKL MFFSLRNQLK DSLEQAKWLE PAARQFIQKK LNEMRLQFGI PDEVLQQPTY
LAQYYNELIL NNVFFVEHLE WIWTFRRSQM EKKLGPLAIL DVIVSEMYTR DNPQAIAYSN
KLNMLLVSKV LISSNYYDYR YPVAVNFARI GTDILEALID NFSTFLLQFN TQSTDISDAA
PEIRYAQPDV NCLAAGQPPR LVHELNELSS NALKSFHVTL SAARTAARAL SNFVNAIDAG
SAIQGSGIDQ ANTYEALGLT RRLRIPGLRS FNENELFTLS YMQQHCSTTI ADKDYARIKP
LVESQLAESY LFNATWQHIQ FLPRSTNCAA AEATCSNLL
