GOGA4_MOUSE
ID GOGA4_MOUSE Reviewed; 2238 AA.
AC Q91VW5; O70365; Q8CGH6;
DT 03-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT 03-JUL-2003, sequence version 2.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Golgin subfamily A member 4;
DE AltName: Full=tGolgin-1;
GN Name=Golga4;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND SUBCELLULAR LOCATION.
RC STRAIN=BALB/cJ; TISSUE=Brain;
RX PubMed=12162805; DOI=10.1089/104454902320219068;
RA Cowan D.A., Gay D., Bieler B.M., Zhao H., Yoshino A., Davis J.G.,
RA Tomayko M.M., Murali R., Greene M.I., Marks M.S.;
RT "Characterization of mouse tGolgin-1 (golgin-245/trans-Golgi p230/256 kD
RT golgin) and its upregulation during oligodendrocyte development.";
RL DNA Cell Biol. 21:505-517(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-980 AND 991-2231, AND VARIANTS
RP ARG-61; SER-280; GLU-293; SER-638; THR-819; THR-829 AND ALA-859.
RC STRAIN=C57BL/6J, and Czech II; TISSUE=Brain, and Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PROTEIN SEQUENCE OF 1840-1856, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC STRAIN=OF1; TISSUE=Hippocampus;
RA Lubec G., Sunyer B., Chen W.-Q.;
RL Submitted (JAN-2009) to UniProtKB.
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT "Large-scale phosphorylation analysis of mouse liver.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-39, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas,
RC Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [6]
RP INTERACTION WITH TBC1D23.
RX PubMed=29084197; DOI=10.1038/ncb3627;
RA Shin J.J.H., Gillingham A.K., Begum F., Chadwick J., Munro S.;
RT "TBC1D23 is a bridging factor for endosomal vesicle capture by golgins at
RT the trans-Golgi.";
RL Nat. Cell Biol. 19:1424-1432(2017).
CC -!- FUNCTION: Involved in vesicular trafficking at the Golgi apparatus
CC level. May play a role in delivery of transport vesicles containing
CC GPI-linked proteins from the trans-Golgi network through its
CC interaction with MACF1. Involved in endosome-to-Golgi trafficking.
CC {ECO:0000250|UniProtKB:Q13439}.
CC -!- SUBUNIT: Homodimer. Interacts with GTP-bound ARL1 and ARL3 (By
CC similarity). Interacts with MACF1 (By similarity). Directly interacts
CC with TBC1D23 (PubMed:29084197). Interacts with FAM91A1; this
CC interaction may be mediated by TBC1D23 (By similarity).
CC {ECO:0000250|UniProtKB:Q13439, ECO:0000269|PubMed:29084197}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:12162805}. Golgi
CC apparatus membrane {ECO:0000269|PubMed:12162805}; Peripheral membrane
CC protein {ECO:0000269|PubMed:12162805}. Golgi apparatus, trans-Golgi
CC network membrane {ECO:0000250|UniProtKB:Q13439}.
CC -!- TISSUE SPECIFICITY: Ubiquitous. Highly expressed in oligodendrocyte
CC precursors, particularly at a stage just prior to myelination.
CC {ECO:0000269|PubMed:12162805}.
CC -!- DOMAIN: Extended rod-like protein with coiled-coil domains.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH37641.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AF051357; AAC05573.2; -; mRNA.
DR EMBL; BC007485; AAH07485.1; -; mRNA.
DR EMBL; BC037641; AAH37641.1; ALT_INIT; mRNA.
DR EMBL; BC053000; AAH53000.1; -; mRNA.
DR CCDS; CCDS40801.1; -.
DR PIR; T14265; T14265.
DR RefSeq; NP_061218.2; NM_018748.3.
DR AlphaFoldDB; Q91VW5; -.
DR SMR; Q91VW5; -.
DR BioGRID; 207604; 8.
DR IntAct; Q91VW5; 3.
DR STRING; 10090.ENSMUSP00000081880; -.
DR iPTMnet; Q91VW5; -.
DR PhosphoSitePlus; Q91VW5; -.
DR EPD; Q91VW5; -.
DR jPOST; Q91VW5; -.
DR MaxQB; Q91VW5; -.
DR PaxDb; Q91VW5; -.
DR PeptideAtlas; Q91VW5; -.
DR PRIDE; Q91VW5; -.
DR ProteomicsDB; 271028; -.
DR Antibodypedia; 28305; 93 antibodies from 23 providers.
DR DNASU; 54214; -.
DR Ensembl; ENSMUST00000084820; ENSMUSP00000081880; ENSMUSG00000038708.
DR GeneID; 54214; -.
DR KEGG; mmu:54214; -.
DR UCSC; uc009rzs.1; mouse.
DR CTD; 2803; -.
DR MGI; MGI:1859646; Golga4.
DR VEuPathDB; HostDB:ENSMUSG00000038708; -.
DR eggNOG; ENOG502QTM0; Eukaryota.
DR GeneTree; ENSGT00730000111139; -.
DR HOGENOM; CLU_001994_0_0_1; -.
DR InParanoid; Q91VW5; -.
DR OMA; DEFRNQG; -.
DR OrthoDB; 663248at2759; -.
DR PhylomeDB; Q91VW5; -.
DR TreeFam; TF325082; -.
DR Reactome; R-MMU-6811440; Retrograde transport at the Trans-Golgi-Network.
DR BioGRID-ORCS; 54214; 2 hits in 72 CRISPR screens.
DR ChiTaRS; Golga4; mouse.
DR PRO; PR:Q91VW5; -.
DR Proteomes; UP000000589; Chromosome 9.
DR RNAct; Q91VW5; protein.
DR Bgee; ENSMUSG00000038708; Expressed in hindlimb stylopod muscle and 274 other tissues.
DR ExpressionAtlas; Q91VW5; baseline and differential.
DR Genevisible; Q91VW5; MM.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005794; C:Golgi apparatus; IDA:MGI.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0051020; F:GTPase binding; ISO:MGI.
DR GO; GO:0031267; F:small GTPase binding; IBA:GO_Central.
DR GO; GO:0043001; P:Golgi to plasma membrane protein transport; ISS:UniProtKB.
DR GO; GO:0048193; P:Golgi vesicle transport; IBA:GO_Central.
DR GO; GO:0045773; P:positive regulation of axon extension; ISO:MGI.
DR InterPro; IPR000237; GRIP_dom.
DR Pfam; PF01465; GRIP; 1.
DR SMART; SM00755; Grip; 1.
DR PROSITE; PS50913; GRIP; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Cytoplasm; Direct protein sequencing; Golgi apparatus;
KW Membrane; Phosphoprotein; Reference proteome.
FT CHAIN 1..2238
FT /note="Golgin subfamily A member 4"
FT /id="PRO_0000190060"
FT DOMAIN 2178..2225
FT /note="GRIP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00250"
FT REGION 1..90
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 132..154
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 154..224
FT /note="Interaction with MACF1"
FT /evidence="ECO:0000250"
FT REGION 1695..1744
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1770..1789
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 156..2161
FT /evidence="ECO:0000255"
FT COMPBIAS 11..89
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1695..1714
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 10
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q13439"
FT MOD_RES 39
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 41
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q13439"
FT MOD_RES 93
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q13439"
FT MOD_RES 100
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q13439"
FT VARIANT 61
FT /note="T -> R (in strain: Czech II)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT VARIANT 280
FT /note="G -> S (in strain: Czech II)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT VARIANT 293
FT /note="G -> E (in strain: Czech II)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT VARIANT 638
FT /note="G -> S (in strain: Czech II)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT VARIANT 819
FT /note="K -> T (in strain: Czech II)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT VARIANT 829
FT /note="A -> T (in strain: Czech II)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT VARIANT 859
FT /note="T -> A (in strain: Czech II)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT VARIANT 2065
FT /note="R -> S (in strain: C57BL/6)"
SQ SEQUENCE 2238 AA; 257564 MW; 494EA2C111F0165B CRC64;
MFKKLKQKIS EEQQQLQQAL APAQASSSSS TPTRTRSRTS SFTDQLDDVT PNRENASTQA
TKSPDGVSKD ESSPSQSGDT QTFAQKLQLR VPSMESLFRS PIKESLFRSS KEPLVRTSSR
ESLNQLDLDC SAAAFDPPSD MESEAEDAPW NSDGLSREQL LQRLRRMERS LSSYRGKYSE
LVTAFQTLQR EKKKLQGILS QSQDKSLRRI SELREELQMD QQAKKHLQDE FDACLEEKDQ
YISVLQTQVS LLKQRLQNGP MNVDAPKPLP PGELQAEVHG DTEKMEGVGE PVGGGTSAKT
LEMLQQRVKR QENLLQRCKE TIGSHKEQCA LLLSEKEALQ EQLDERLQEL EKMKELHMAE
KTKLITQLRD AKNLIEQLEQ DKGMVITETK RQMLETLELK EDEIAQLRSH IKQMTTQGEE
LREQKEKSER AAFEELEKAL STAQKTEDAQ RRMKMEMDEQ MKAVERASEE ERLRLQHELS
RVRQEAASMA KKNSEEQVAA LQKLHAEELA SKEQELSRRL EARERELQEQ MRIALEKSRS
EYLKLTQEKE QQESLALEEL ELQKKAILTE SENKLQELGQ EAEAYRTRIL ELETSLEKSL
QESKTQSEHL AVHLEAEKNK HNKELTALAE QHRTEVEGLQ QQQDSLWTER LQSLSQQHQA
AVEELREKYQ QEKDALLKEK ESLFQAHIQD MNEKTLEKLD KKQMELESVS SELSEALRAR
DQLAEELSVL RGDADKMKQA LEAELEEQRR HHQREVGSIS EQQELTVRRA EKALKDELSR
LGALLDERDE HLRERQARVQ DLEAHLQKSA GELQQALAKL DLLHSEQSAA REQAGAYEEQ
LAQMQQKVLD LETEKSLLTK QVVEMETHKK HVCEELDAQR AQVQQLERQR SELEEKVRSL
AQLQDSQLKN STVEKEQARQ SLMEKENIIL QMREEQAKEI EILKQTLSSK EESISILHEE
YETKFKNQEK RMEKIKQKAK EMQETKKKLL DQEAKLKKEL ENTVLELSQK EKQFNAQILE
MAQANSAGIS DTVSRLEENQ RQQIESLTGA HQRKLDDVIE AWEKKLSQQA AELRDKHAEQ
MEEKEQGLGE LRQKVRIVQS EKEELTKEVA RLKEAVSGQD VALAGLQGQL EQKSAVIVSL
SERESQLQSQ VEKLEADLGC SLSEKLSLQE ELAELKLLAD KSQLRVSELT GQVQAAEKEL
QSCKSLHELS KKSLEDKSLN LKSLLEELAS QLDSRCERTK ALLEAKTNEL VCTSRDKADA
ILARLSQCQR HTATVGEALL RRMGQVSELE AQLTQLTEEQ RTLKSSFQQV TNQLEEKEKQ
IKTMKADIEG LLTEKEALQQ EGGQQRQAAS EKESCITQLK KELAENINAV TLLREELSEK
KSEIASLSKQ LSDLGAQLES SISPSDKAEA ISALSKQHEE QELQLQAQLQ ELSLKVDALS
KEKMSALEQV DHWSNKFSEW KKKAQSRLAQ HQSTIKDLQA QLDVKATDAR EKEEQICLLK
EDLDRQNKKF ECLKGEMEVR KSKMEKKECD LETALKTQTA RVVELEDCVT QRKKEVESLN
ETLKNYNQQR DTEHSGLVQR LQHLEELGEE KDNKVREAEE TVLRLREHVS SLEAELGTVK
KELEHVNSSV KSRDGELKAL EDKLELESAA KVELKRKAEQ KIAAIRKQLL SQMEEKTQRY
AKDTENRLSE LSAQLKEREK QVHSLEDKLK NLESSPHPEV PAVSRSMQSV AASPEQEAPD
SQDCTHKACK ERLCMLQRRL SEKEKLLRRL EQGEGEARPS QPEAQHRALS GKLDCTRARQ
LEDHVLIGCL PEELEEKMKC SLIVSQPMGE ETGNNTGVKQ NWASVVDSVQ KTLQEKELTC
QALEQRVKEL ESDLVRERGA HRLEVEKLTL KYEKSQSSQQ EMDGENKCVE VLEDRPEENS
QSHEIQSNVG TVDGLRSDLE SKLTGAERDK QKLSKEVARL QKELRALRRE HQQELDILKR
ECEQEAEEKL KQEQEDLELK HTSTLKQLMR EFNTQLAQKE QELERTVQET IDKAQEVEAE
LLESHQEETQ QLHRKIAEKE DDLRRTARRY EEILDAREEE MTGKVTDLQT QLEELQKKYQ
QRLEQEESTK DSVTILELQT QLAQKTTLIS DSKLKEQELR EQVHNLEDRL KRYEKNACAA
TVGTPYKGGN LYHTEVSLFG EPTEFEYLRK VMFEYMMGRE TKTMAKVITT VLKFPDDQAQ
KILEREDARL MSWLRTSS
