GOLI4_MOUSE
ID GOLI4_MOUSE Reviewed; 655 AA.
AC Q8BXA1; Q8BWP9;
DT 01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Golgi integral membrane protein 4;
DE AltName: Full=Decapacitation factor 10;
DE AltName: Full=Golgi phosphoprotein 4;
GN Name=Golim4; Synonyms=Df10, Golph4;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Head, and Liver;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Fetal brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND TISSUE SPECIFICITY.
RX PubMed=16221991; DOI=10.1095/biolreprod.105.044644;
RA Nixon B., MacIntyre D.A., Mitchell L.A., Gibbs G.M., O'Bryan M.,
RA Aitken R.J.;
RT "The identification of mouse sperm-surface-associated proteins and
RT characterization of their ability to act as decapacitation factors.";
RL Biol. Reprod. 74:275-287(2006).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-633, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain;
RX PubMed=18034455; DOI=10.1021/pr0701254;
RA Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.;
RT "Large-scale identification and evolution indexing of tyrosine
RT phosphorylation sites from murine brain.";
RL J. Proteome Res. 7:311-318(2008).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brown adipose tissue, Heart, Liver, Lung, Pancreas, Spleen, and
RC Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Plays a role in endosome to Golgi protein trafficking;
CC mediates protein transport along the late endosome-bypass pathway from
CC the early endosome to the Golgi. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus, Golgi stack membrane
CC {ECO:0000250}; Single-pass type II membrane protein {ECO:0000250}.
CC Endosome membrane {ECO:0000250}; Single-pass type II membrane protein
CC {ECO:0000250}. Membrane {ECO:0000250|UniProtKB:O00461}; Lipid-anchor
CC {ECO:0000250|UniProtKB:O00461}. Note=Localizes to cis and medial Golgi
CC cisternae. Probably cycles between early Golgi and distal compartments
CC like endosome (By similarity). {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed by spermatozoa (at protein level).
CC {ECO:0000269|PubMed:16221991}.
CC -!- PTM: Phosphorylated by c-AMP-dependent kinases most probably in its
CC lumenal part. {ECO:0000250}.
CC -!- PTM: O-glycosylated; modified by sialic acid residues. {ECO:0000250}.
CC -!- PTM: N-glycosylated; N-glycans are of the complex type and modified by
CC sialic acid residues. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the GOLIM4 family. {ECO:0000305}.
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DR EMBL; AK048434; BAC33335.1; -; mRNA.
DR EMBL; AK050346; BAC34202.1; -; mRNA.
DR EMBL; BC086642; AAH86642.1; -; mRNA.
DR EMBL; BC089354; AAH89354.1; -; mRNA.
DR CCDS; CCDS17416.1; -.
DR RefSeq; NP_780402.1; NM_175193.6.
DR AlphaFoldDB; Q8BXA1; -.
DR SMR; Q8BXA1; -.
DR BioGRID; 215783; 2.
DR STRING; 10090.ENSMUSP00000048997; -.
DR GlyGen; Q8BXA1; 1 site.
DR iPTMnet; Q8BXA1; -.
DR PhosphoSitePlus; Q8BXA1; -.
DR SwissPalm; Q8BXA1; -.
DR CPTAC; non-CPTAC-4030; -.
DR EPD; Q8BXA1; -.
DR jPOST; Q8BXA1; -.
DR MaxQB; Q8BXA1; -.
DR PaxDb; Q8BXA1; -.
DR PeptideAtlas; Q8BXA1; -.
DR PRIDE; Q8BXA1; -.
DR ProteomicsDB; 267646; -.
DR Antibodypedia; 952; 126 antibodies from 20 providers.
DR DNASU; 73124; -.
DR Ensembl; ENSMUST00000038563; ENSMUSP00000048997; ENSMUSG00000034109.
DR Ensembl; ENSMUST00000167078; ENSMUSP00000132910; ENSMUSG00000034109.
DR GeneID; 73124; -.
DR KEGG; mmu:73124; -.
DR UCSC; uc008pnf.2; mouse.
DR CTD; 27333; -.
DR MGI; MGI:1920374; Golim4.
DR VEuPathDB; HostDB:ENSMUSG00000034109; -.
DR eggNOG; ENOG502R4Q5; Eukaryota.
DR GeneTree; ENSGT00390000004096; -.
DR InParanoid; Q8BXA1; -.
DR PhylomeDB; Q8BXA1; -.
DR TreeFam; TF333101; -.
DR BioGRID-ORCS; 73124; 5 hits in 74 CRISPR screens.
DR ChiTaRS; Golim4; mouse.
DR PRO; PR:Q8BXA1; -.
DR Proteomes; UP000000589; Chromosome 3.
DR RNAct; Q8BXA1; protein.
DR Bgee; ENSMUSG00000034109; Expressed in vault of skull and 250 other tissues.
DR ExpressionAtlas; Q8BXA1; baseline and differential.
DR Genevisible; Q8BXA1; MM.
DR GO; GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005794; C:Golgi apparatus; ISO:MGI.
DR GO; GO:0032580; C:Golgi cisterna membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0000139; C:Golgi membrane; IEA:InterPro.
DR GO; GO:0016021; C:integral component of membrane; ISO:MGI.
DR InterPro; IPR042336; GOLIM4.
DR PANTHER; PTHR22909; PTHR22909; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Endosome; Glycoprotein; Golgi apparatus; Lipoprotein;
KW Membrane; Myristate; Phosphoprotein; Reference proteome; Signal-anchor;
KW Transmembrane; Transmembrane helix; Transport.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:O00461"
FT CHAIN 2..655
FT /note="Golgi integral membrane protein 4"
FT /id="PRO_0000285098"
FT TOPO_DOM 2..12
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 13..33
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 34..655
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT REGION 38..107
FT /note="Golgi targeting"
FT /evidence="ECO:0000250"
FT REGION 80..175
FT /note="Endosome targeting"
FT /evidence="ECO:0000250"
FT REGION 122..145
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 176..220
FT /note="Golgi targeting"
FT /evidence="ECO:0000250"
FT REGION 256..275
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 285..655
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 66..216
FT /evidence="ECO:0000255"
FT COMPBIAS 258..272
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 288..340
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 358..374
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 376..399
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 403..420
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 464..511
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 516..536
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 537..554
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 571..586
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 587..633
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 328
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O00461"
FT MOD_RES 540
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q5BJK8"
FT MOD_RES 576
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000250|UniProtKB:O00461"
FT MOD_RES 589
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:O00461"
FT MOD_RES 633
FT /note="Phosphotyrosine"
FT /evidence="ECO:0007744|PubMed:18034455"
FT LIPID 2
FT /note="N-myristoyl glycine"
FT /evidence="ECO:0000250|UniProtKB:O00461"
FT CARBOHYD 229
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 655 AA; 76785 MW; 273CD26423D05CA7 CRC64;
MGNGMCSRKQ KRIFQTLLLL TVVFGFLYGA MLYLELQTQL RKAEAVALKY QQHQDSLSAQ
LQVVYEHRSR LEKSLQKERL EHKKAKEDFL VYKLEAQETL NKGRQDSNSR YSALNVQHQM
LKSQHEELRK QHSDLEEEHR KQGEDFSRTF NDHKQRYLQL QQEKEQELSK LKETVYNLRE
ENRQLRKAHQ DIHTQLQDVK TQVAEYKQLK DTLNRIPSFR NPDPVEQQNV TFPHGTHPPQ
GYNGREKLTG ELQEVQPNHE AGPRRMEEKP LSSMQKDAGF QALEEQNQVE PREPEERQVE
EEHRKALEEE EMEQVGQAEH LEEEHDPSPE EQDRDWRDQQ GQNAAHLLDG HPQAEVEHST
KAATNFQSPY EEQLEQQRLA ARRDEEAQRL REHQEALHQQ RLHGQLLRQQ QQQQFLAREM
AQQKQVAHED GQQQHQEQLR QQAHYNAVEN DIAQGVEDQG IPEEEGGAYD RDNQRQDEAE
GDPGNRQELR EPGHQEGDPE VEADRAAVED INPADDPNNQ GEDEFEEAEQ VREENLPEES
EEQKQSEAKQ GNVEMDEHLV MAGNPDQQED NVDEQYQDEG EEEVQEDLTE EKKREMEHNV
EETYGEHPDD KNNDGEEQGV HNRAHPKGRQ EHYEEEEDEE DGAAVAEKSH RRAEM
