GOLM2_HUMAN
ID GOLM2_HUMAN Reviewed; 436 AA.
AC Q6P4E1; B4DPZ6; G5E934; Q6UY45; Q96EM1;
DT 26-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT 05-JUN-2019, sequence version 2.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Protein GOLM2 {ECO:0000305};
DE AltName: Full=Cancer susceptibility candidate gene 4 protein;
DE Short=CASC4;
DE AltName: Full=Golgi membrane protein 2 {ECO:0000305};
GN Name=GOLM2 {ECO:0000312|HGNC:HGNC:24892}; Synonyms=CASC4;
GN ORFNames=UNQ2573/PRO6308;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RX PubMed=12975309; DOI=10.1101/gr.1293003;
RA Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S., Huang A.,
RA Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D.,
RA Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L.,
RA Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C.,
RA Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J.,
RA Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.;
RT "The secreted protein discovery initiative (SPDI), a large-scale effort to
RT identify novel human secreted and transmembrane proteins: a bioinformatics
RT assessment.";
RL Genome Res. 13:2265-2270(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 5).
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16572171; DOI=10.1038/nature04601;
RA Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K.,
RA Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K.,
RA FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N.,
RA Abouelleil A., Arachchi H.M., Baradarani L., Birditt B., Bloom S.,
RA Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K.,
RA DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J.,
RA Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E.,
RA Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B.,
RA Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R.,
RA O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B.,
RA Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S.,
RA Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C.;
RT "Analysis of the DNA sequence and duplication history of human chromosome
RT 15.";
RL Nature 440:671-675(2006).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC TISSUE=Blood, and Kidney;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-366, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=20068231; DOI=10.1126/scisignal.2000475;
RA Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L.,
RA Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.;
RT "Quantitative phosphoproteomics reveals widespread full phosphorylation
RT site occupancy during mitosis.";
RL Sci. Signal. 3:RA3-RA3(2010).
RN [8]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
RN [9]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-332 AND SER-366, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
RN [10]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-233; SER-275; SER-328;
RP SER-332 AND SER-366, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE
RP ANALYSIS].
RC TISSUE=Liver;
RX PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA Ye M., Zou H.;
RT "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT phosphoproteome.";
RL J. Proteomics 96:253-262(2014).
RN [11]
RP ACETYLATION AT MET-1.
RX PubMed=25732826; DOI=10.1016/j.celrep.2015.01.053;
RA Aksnes H., Van Damme P., Goris M., Starheim K.K., Marie M., Stoeve S.I.,
RA Hoel C., Kalvik T.V., Hole K., Glomnes N., Furnes C., Ljostveit S.,
RA Ziegler M., Niere M., Gevaert K., Arnesen T.;
RT "An organellar nalpha-acetyltransferase, naa60, acetylates cytosolic N
RT termini of transmembrane proteins and maintains Golgi integrity.";
RL Cell Rep. 10:1362-1374(2015).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type II
CC membrane protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=4;
CC IsoId=Q6P4E1-4; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6P4E1-2; Sequence=VSP_060181;
CC Name=3;
CC IsoId=Q6P4E1-3; Sequence=VSP_060179, VSP_060180;
CC Name=5;
CC IsoId=Q6P4E1-5; Sequence=VSP_060178;
CC -!- SIMILARITY: Belongs to the GOLM family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH63480.1; Type=Miscellaneous discrepancy; Note=Probable cloning artifact.; Evidence={ECO:0000305};
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DR EMBL; AY358086; AAQ88453.1; -; mRNA.
DR EMBL; AK298564; BAG60758.1; -; mRNA.
DR EMBL; AL832333; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AC025043; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC090519; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471082; EAW77258.1; -; Genomic_DNA.
DR EMBL; BC012124; AAH12124.1; -; mRNA.
DR EMBL; BC063480; AAH63480.1; ALT_SEQ; mRNA.
DR CCDS; CCDS10108.1; -. [Q6P4E1-4]
DR CCDS; CCDS10109.1; -. [Q6P4E1-2]
DR RefSeq; NP_612432.2; NM_138423.3. [Q6P4E1-4]
DR RefSeq; NP_816929.1; NM_177974.2. [Q6P4E1-2]
DR AlphaFoldDB; Q6P4E1; -.
DR SMR; Q6P4E1; -.
DR BioGRID; 125233; 41.
DR IntAct; Q6P4E1; 10.
DR MINT; Q6P4E1; -.
DR STRING; 9606.ENSP00000299957; -.
DR GlyConnect; 1652; 4 N-Linked glycans (1 site).
DR GlyGen; Q6P4E1; 11 sites, 3 N-linked glycans (1 site), 3 O-linked glycans (6 sites).
DR iPTMnet; Q6P4E1; -.
DR PhosphoSitePlus; Q6P4E1; -.
DR BioMuta; CASC4; -.
DR DMDM; 74737334; -.
DR EPD; Q6P4E1; -.
DR jPOST; Q6P4E1; -.
DR MassIVE; Q6P4E1; -.
DR MaxQB; Q6P4E1; -.
DR PaxDb; Q6P4E1; -.
DR PeptideAtlas; Q6P4E1; -.
DR PRIDE; Q6P4E1; -.
DR ProteomicsDB; 33815; -.
DR ProteomicsDB; 66964; -. [Q6P4E1-2]
DR ProteomicsDB; 66965; -. [Q6P4E1-3]
DR Antibodypedia; 55771; 89 antibodies from 18 providers.
DR DNASU; 113201; -.
DR Ensembl; ENST00000299957.11; ENSP00000299957.6; ENSG00000166734.20. [Q6P4E1-4]
DR Ensembl; ENST00000345795.6; ENSP00000335063.4; ENSG00000166734.20. [Q6P4E1-2]
DR Ensembl; ENST00000557945.5; ENSP00000453720.1; ENSG00000166734.20. [Q6P4E1-3]
DR GeneID; 113201; -.
DR KEGG; hsa:113201; -.
DR MANE-Select; ENST00000299957.11; ENSP00000299957.6; NM_138423.4; NP_612432.2.
DR UCSC; uc001ztp.4; human. [Q6P4E1-4]
DR CTD; 113201; -.
DR DisGeNET; 113201; -.
DR GeneCards; GOLM2; -.
DR HGNC; HGNC:24892; GOLM2.
DR HPA; ENSG00000166734; Low tissue specificity.
DR neXtProt; NX_Q6P4E1; -.
DR OpenTargets; ENSG00000166734; -.
DR VEuPathDB; HostDB:ENSG00000166734; -.
DR eggNOG; ENOG502QTYH; Eukaryota.
DR GeneTree; ENSGT00530000063675; -.
DR HOGENOM; CLU_129925_0_0_1; -.
DR InParanoid; Q6P4E1; -.
DR OMA; ERGPGLH; -.
DR OrthoDB; 913812at2759; -.
DR PhylomeDB; Q6P4E1; -.
DR TreeFam; TF331127; -.
DR PathwayCommons; Q6P4E1; -.
DR SignaLink; Q6P4E1; -.
DR BioGRID-ORCS; 113201; 15 hits in 1080 CRISPR screens.
DR ChiTaRS; CASC4; human.
DR GenomeRNAi; 113201; -.
DR Pharos; Q6P4E1; Tbio.
DR PRO; PR:Q6P4E1; -.
DR Proteomes; UP000005640; Chromosome 15.
DR RNAct; Q6P4E1; protein.
DR Bgee; ENSG00000166734; Expressed in calcaneal tendon and 186 other tissues.
DR ExpressionAtlas; Q6P4E1; baseline and differential.
DR Genevisible; Q6P4E1; HS.
DR GO; GO:0005794; C:Golgi apparatus; IDA:HPA.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR InterPro; IPR026141; CASC4.
DR InterPro; IPR026139; GOLM1/CASC4.
DR PANTHER; PTHR15896; PTHR15896; 1.
DR PANTHER; PTHR15896:SF11; PTHR15896:SF11; 1.
DR PRINTS; PR02084; GOLM1CASC4.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Coiled coil; Membrane; Phosphoprotein;
KW Reference proteome; Signal-anchor; Transmembrane; Transmembrane helix.
FT CHAIN 1..436
FT /note="Protein GOLM2"
FT /id="PRO_0000291843"
FT TOPO_DOM 1..14
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 15..35
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 36..436
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT REGION 225..436
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 35..198
FT /evidence="ECO:0000255"
FT COMPBIAS 225..245
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 276..339
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 340..369
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 386..400
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 401..421
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000269|PubMed:25732826,
FT ECO:0007744|PubMed:22814378"
FT MOD_RES 233
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:24275569"
FT MOD_RES 275
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:24275569"
FT MOD_RES 328
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:24275569"
FT MOD_RES 332
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163,
FT ECO:0007744|PubMed:24275569"
FT MOD_RES 366
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:20068231,
FT ECO:0007744|PubMed:23186163, ECO:0007744|PubMed:24275569"
FT VAR_SEQ 1..22
FT /note="MVGFGANRRAGRLPSLVLVVLL -> M (in isoform 5)"
FT /id="VSP_060178"
FT VAR_SEQ 163..177
FT /note="FQCGQQMKELRAQHE -> KRPKRFNQMMERNWI (in isoform 3)"
FT /id="VSP_060179"
FT VAR_SEQ 178..436
FT /note="Missing (in isoform 3)"
FT /id="VSP_060180"
FT VAR_SEQ 358..414
FT /note="SRFFDENESPVDPQHGSKLADYNGDDGNVGEYEADKQAELAYNEEEDGDGGE
FT EDVQD -> N (in isoform 2)"
FT /id="VSP_060181"
FT CONFLICT 429
FT /note="K -> E (in Ref. 3)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 436 AA; 49498 MW; E11DABFDD27CF70A CRC64;
MVGFGANRRA GRLPSLVLVV LLVVIVVLAF NYWSISSRHV LLQEEVAELQ GQVQRTEVAR
GRLEKRNSDL LLLVDTHKKQ IDQKEADYGR LSSRLQAREG LGKRCEDDKV KLQNNISYQM
ADIHHLKEQL AELRQEFLRQ EDQLQDYRKN NTYLVKRLEY ESFQCGQQMK ELRAQHEENI
KKLADQFLEE QKQETQKIQS NDGKELDINN QVVPKNIPKV AENVADKNEE PSSNHIPHGK
EQIKRGGDAG MPGIEENDLA KVDDLPPALR KPPISVSQHE SHQAISHLPT GQPLSPNMPP
DSHINHNGNP GTSKQNPSSP LQRLIPGSNL DSEPRIQTDI LKQATKDRVS DFHKLKQSRF
FDENESPVDP QHGSKLADYN GDDGNVGEYE ADKQAELAYN EEEDGDGGEE DVQDDEEREL
QMDPADYGKQ HFNDVL
