ID   GOLS1_SOLLC             Reviewed;         318 AA.
AC   Q947G8;
DT   05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   03-AUG-2022, entry version 76.
DE   RecName: Full=Galactinol synthase 1;
DE            Short=GolS-1;
DE            Short=SlGolS1;
DE            EC=2.4.1.123;
GN   Name=GOLS1;
OS   Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum;
OC   Solanum subgen. Lycopersicon.
OX   NCBI_TaxID=4081;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, DEVELOPMENTAL STAGE,
RP   TISSUE SPECIFICITY, AND INDUCTION.
RC   STRAIN=cv. Moneymaker;
RX   PubMed=12644684; DOI=10.1104/pp.016386;
RA   Downie B., Gurusinghe S., Dahal P., Thacker R.R., Snyder J.C., Nonogaki H.,
RA   Yim K., Fukanaga K., Alvarado V., Bradford K.J.;
RT   "Expression of a GALACTINOL SYNTHASE gene in tomato seeds is up-regulated
RT   before maturation desiccation and again after imbibition whenever radicle
RT   protrusion is prevented.";
RL   Plant Physiol. 131:1347-1359(2003).
CC   -!- FUNCTION: Galactinol synthase involved in the biosynthesis of raffinose
CC       family oligosaccharides (RFOs) that function as osmoprotectants. May
CC       promote plant stress tolerance. {ECO:0000269|PubMed:12644684}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=myo-inositol + UDP-alpha-D-galactose = alpha-D-galactosyl-
CC         (1->3)-1D-myo-inositol + H(+) + UDP; Xref=Rhea:RHEA:12464,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:17268, ChEBI:CHEBI:17505,
CC         ChEBI:CHEBI:58223, ChEBI:CHEBI:66914; EC=2.4.1.123;
CC   -!- COFACTOR:
CC       Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC         Evidence={ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Expressed in seeds, mostly in radicle tips.
CC       {ECO:0000269|PubMed:12644684}.
CC   -!- DEVELOPMENTAL STAGE: Accumulates in developing seeds during dry weight
CC       deposition and desiccation tolerance acquisition. Disappears after
CC       germination. {ECO:0000269|PubMed:12644684}.
CC   -!- INDUCTION: Declines within 8 hours of imbibition in a gibberellin-
CC       dependent manner. Induced by dehydration but not by cold in germinating
CC       seeds, and induced by both dehydration and cold in leaves.
CC       {ECO:0000269|PubMed:12644684}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 8 family.
CC       Galactosyltransferase subfamily. {ECO:0000305}.
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DR   EMBL; AF311943; AAL26804.1; -; mRNA.
DR   EMBL; AF447452; AAO72744.1; -; Genomic_DNA.
DR   RefSeq; NP_001234486.2; NM_001247557.2.
DR   AlphaFoldDB; Q947G8; -.
DR   SMR; Q947G8; -.
DR   STRING; 4081.Solyc01g100830.1.1; -.
DR   CAZy; GT8; Glycosyltransferase Family 8.
DR   PaxDb; Q947G8; -.
DR   PRIDE; Q947G8; -.
DR   GeneID; 544174; -.
DR   KEGG; sly:544174; -.
DR   eggNOG; KOG1950; Eukaryota.
DR   InParanoid; Q947G8; -.
DR   OrthoDB; 818680at2759; -.
DR   BRENDA; 2.4.1.123; 3101.
DR   Proteomes; UP000004994; Unplaced.
DR   ExpressionAtlas; Q947G8; baseline.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016757; F:glycosyltransferase activity; IBA:GO_Central.
DR   GO; GO:0047216; F:inositol 3-alpha-galactosyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0070417; P:cellular response to cold; IEP:UniProtKB.
DR   GO; GO:0071465; P:cellular response to desiccation; IEP:UniProtKB.
DR   GO; GO:0006012; P:galactose metabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.90.550.10; -; 1.
DR   InterPro; IPR002495; Glyco_trans_8.
DR   InterPro; IPR030515; GOLS.
DR   InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR   PANTHER; PTHR11183:SF143; PTHR11183:SF143; 1.
DR   Pfam; PF01501; Glyco_transf_8; 1.
DR   SUPFAM; SSF53448; SSF53448; 1.
PE   2: Evidence at transcript level;
KW   Carbohydrate metabolism; Cytoplasm; Galactose metabolism;
KW   Glycosyltransferase; Manganese; Metal-binding; Reference proteome;
KW   Transferase.
FT   CHAIN           1..318
FT                   /note="Galactinol synthase 1"
FT                   /id="PRO_0000418653"
FT   ACT_SITE        102
FT                   /evidence="ECO:0000250"
FT   BINDING         118
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000250"
FT   BINDING         120
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000250"
FT   BINDING         244
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   318 AA;  36402 MW;  AE66D71E45097E52 CRC64;
     MAPEFESGTK MATTIQKSSC AYVTFLAGNG DYVKGVVGLA KGLIKAKSMY PLVVAILPDV
     PEEHRMILTR HGCIVKEIEP LAPSLQSLDK YARSYYVLNY SKLRIWEFVE YSKMVYLDGD
     MQVFENIDHL FELPDKYLYA VADCICDMYG EPCDEVLPWP KELGPRPSVY FNAGMFVFQP
     NPSVYVRLLN TLKVTPPTQF AEQDFLNMYF KDVYKPIPYT YNMLLAMLWR HPEKIEVNKA
     KAVHYCSPGA KPWKYTGKEE HMDREDIKML VKKWWDIYND TTLDHKAQGS TVEANRLRGA
     AFSDTNISAL YITSPSAA