ID   GOLS2_SOLLC             Reviewed;         338 AA.
AC   C7G304;
DT   05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT   13-OCT-2009, sequence version 1.
DT   03-AUG-2022, entry version 50.
DE   RecName: Full=Galactinol synthase 2;
DE            Short=GolS-2;
DE            Short=SlGolS2;
DE            EC=2.4.1.123;
GN   Name=GOLS2;
OS   Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum;
OC   Solanum subgen. Lycopersicon.
OX   NCBI_TaxID=4081;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. MicroTom;
RA   Yuasa T., Iwaya-Inoue M.;
RT   "Tomato galactinol synthase.";
RL   Submitted (FEB-2009) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Galactinol synthase involved in the biosynthesis of raffinose
CC       family oligosaccharides (RFOs) that function as osmoprotectants. May
CC       promote plant stress tolerance (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=myo-inositol + UDP-alpha-D-galactose = alpha-D-galactosyl-
CC         (1->3)-1D-myo-inositol + H(+) + UDP; Xref=Rhea:RHEA:12464,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:17268, ChEBI:CHEBI:17505,
CC         ChEBI:CHEBI:58223, ChEBI:CHEBI:66914; EC=2.4.1.123;
CC   -!- COFACTOR:
CC       Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC         Evidence={ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 8 family.
CC       Galactosyltransferase subfamily. {ECO:0000305}.
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DR   EMBL; AB486014; BAH98060.1; -; mRNA.
DR   RefSeq; NP_001234668.2; NM_001247739.2.
DR   AlphaFoldDB; C7G304; -.
DR   SMR; C7G304; -.
DR   STRING; 4081.Solyc02g084980.2.1; -.
DR   CAZy; GT8; Glycosyltransferase Family 8.
DR   PaxDb; C7G304; -.
DR   PRIDE; C7G304; -.
DR   GeneID; 100316891; -.
DR   KEGG; sly:100316891; -.
DR   eggNOG; KOG1950; Eukaryota.
DR   InParanoid; C7G304; -.
DR   OrthoDB; 818680at2759; -.
DR   Proteomes; UP000004994; Unplaced.
DR   ExpressionAtlas; C7G304; baseline and differential.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016757; F:glycosyltransferase activity; IBA:GO_Central.
DR   GO; GO:0047216; F:inositol 3-alpha-galactosyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006012; P:galactose metabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.90.550.10; -; 1.
DR   InterPro; IPR002495; Glyco_trans_8.
DR   InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR   Pfam; PF01501; Glyco_transf_8; 1.
DR   SUPFAM; SSF53448; SSF53448; 1.
PE   2: Evidence at transcript level;
KW   Carbohydrate metabolism; Cytoplasm; Galactose metabolism;
KW   Glycosyltransferase; Manganese; Metal-binding; Reference proteome;
KW   Transferase.
FT   CHAIN           1..338
FT                   /note="Galactinol synthase 2"
FT                   /id="PRO_0000418654"
FT   ACT_SITE        105
FT                   /evidence="ECO:0000250"
FT   BINDING         121
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000250"
FT   BINDING         123
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000250"
FT   BINDING         258
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   338 AA;  38786 MW;  18E013DF9F1AA61C CRC64;
     MAPNVFGLAT KATGLAKAKS LSSRAYVTFL AGNGDYWKGV VGLVKGLRKA KSAYPLVVAC
     LPDVPEEHRR ILINQGCIVR EIEPVYPPHN QTQFAMAYYV INYSKLRIWE FVEYSKMIYL
     DGDIQVFDNI DHLFDLPDGY FYAVMDCFCE KTWSHTPQYK VGYCQQCPDK VQWTEDLGPK
     PSLYFNAGMF VYEPSLSTYD DLLKTLKVTP PTPFAEQDFL NMYFRDVYKP IPNDYNLVLA
     MLWRHPENVD LEKVKVVHYC AAGSKPWRYT GKEENMDRED IKMLIKKWWD IYDDESLDYK
     NSNVVMNAVD GEVEAQKIME ALSEAGVVHY ITAPSAAS