GOLS5_ARATH
ID GOLS5_ARATH Reviewed; 333 AA.
AC Q9FFA1;
DT 05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Galactinol synthase 5;
DE Short=AtGolS5;
DE Short=GolS-5;
DE EC=2.4.1.123;
GN Name=GOLS5; OrderedLocusNames=At5g23790; ORFNames=MRO11.17;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9330910; DOI=10.1093/dnares/4.3.215;
RA Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M.,
RA Miyajima N., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence
RT features of the 1.6 Mb regions covered by twenty physically assigned P1
RT clones.";
RL DNA Res. 4:215-230(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY.
RX PubMed=11846875; DOI=10.1046/j.0960-7412.2001.01227.x;
RA Taji T., Ohsumi C., Iuchi S., Seki M., Kasuga M., Kobayashi M.,
RA Yamaguchi-Shinozaki K., Shinozaki K.;
RT "Important roles of drought- and cold-inducible genes for galactinol
RT synthase in stress tolerance in Arabidopsis thaliana.";
RL Plant J. 29:417-426(2002).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=18502973; DOI=10.1104/pp.108.122465;
RA Nishizawa A., Yabuta Y., Shigeoka S.;
RT "Galactinol and raffinose constitute a novel function to protect plants
RT from oxidative damage.";
RL Plant Physiol. 147:1251-1263(2008).
CC -!- FUNCTION: Galactinol synthase involved in the biosynthesis of raffinose
CC family oligosaccharides (RFOs) that function as osmoprotectants. May
CC promote plant stress tolerance (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=myo-inositol + UDP-alpha-D-galactose = alpha-D-galactosyl-
CC (1->3)-1D-myo-inositol + H(+) + UDP; Xref=Rhea:RHEA:12464,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:17268, ChEBI:CHEBI:17505,
CC ChEBI:CHEBI:58223, ChEBI:CHEBI:66914; EC=2.4.1.123;
CC -!- COFACTOR:
CC Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC Evidence={ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 8 family.
CC Galactosyltransferase subfamily. {ECO:0000305}.
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DR EMBL; AB005244; BAB10052.1; -; Genomic_DNA.
DR EMBL; CP002688; AED93213.1; -; Genomic_DNA.
DR RefSeq; NP_197768.1; NM_122284.2.
DR AlphaFoldDB; Q9FFA1; -.
DR SMR; Q9FFA1; -.
DR STRING; 3702.AT5G23790.1; -.
DR CAZy; GT8; Glycosyltransferase Family 8.
DR PaxDb; Q9FFA1; -.
DR PRIDE; Q9FFA1; -.
DR EnsemblPlants; AT5G23790.1; AT5G23790.1; AT5G23790.
DR GeneID; 832444; -.
DR Gramene; AT5G23790.1; AT5G23790.1; AT5G23790.
DR KEGG; ath:AT5G23790; -.
DR Araport; AT5G23790; -.
DR TAIR; locus:2172848; AT5G23790.
DR eggNOG; KOG1950; Eukaryota.
DR HOGENOM; CLU_049943_3_0_1; -.
DR InParanoid; Q9FFA1; -.
DR OMA; QIEPVIP; -.
DR OrthoDB; 818680at2759; -.
DR PhylomeDB; Q9FFA1; -.
DR PRO; PR:Q9FFA1; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FFA1; baseline and differential.
DR Genevisible; Q9FFA1; AT.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016757; F:glycosyltransferase activity; IBA:GO_Central.
DR GO; GO:0047216; F:inositol 3-alpha-galactosyltransferase activity; ISS:UniProtKB.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006012; P:galactose metabolic process; ISS:UniProtKB.
DR GO; GO:0006950; P:response to stress; IEA:UniProt.
DR Gene3D; 3.90.550.10; -; 1.
DR InterPro; IPR002495; Glyco_trans_8.
DR InterPro; IPR030515; GOLS.
DR InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR PANTHER; PTHR11183:SF102; PTHR11183:SF102; 1.
DR Pfam; PF01501; Glyco_transf_8; 1.
DR SUPFAM; SSF53448; SSF53448; 1.
PE 2: Evidence at transcript level;
KW Carbohydrate metabolism; Cytoplasm; Galactose metabolism;
KW Glycosyltransferase; Manganese; Metal-binding; Reference proteome;
KW Transferase.
FT CHAIN 1..333
FT /note="Galactinol synthase 5"
FT /id="PRO_0000418661"
FT ACT_SITE 103
FT /evidence="ECO:0000250"
FT BINDING 119
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250"
FT BINDING 121
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250"
FT BINDING 257
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250"
SQ SEQUENCE 333 AA; 38472 MW; EE8BEFB82FBBEAA3 CRC64;
MTMTVEKRIE ADVTVSHEGV ERAYVTFLAG NKDYWMLVVG LAKGLRKVKS AYPLVVATLP
DVPEEHRQIL VDQGCIIRDI EPVYPPENTT GYSMAYYVIN YSKLRIWEFV EYEKMIYLDG
DIQVFKNIDH LFDTPRGYLY AVKDCFCEVS WSKTPQYKIG YCQQSPEKVT WPVESLGAPP
PVYFNAGMLV FGPNLVTYED LLRVVQITTP TYFAEQDFLN IYFRDIYKPI PSTYNLVMAM
LWRHPEHIDL DQISVVHYCA NGSKPWKFDE AEEHMDREDI KMLVKKWWEI YEDSSLDYKN
FVETESKLNP VTATLASKKL VGDVLTSLAP SAA
