GOLS6_ARATH
ID GOLS6_ARATH Reviewed; 336 AA.
AC Q8H1S1; Q9STQ9;
DT 05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Galactinol synthase 6;
DE Short=AtGolS6;
DE Short=GolS-6;
DE EC=2.4.1.123;
GN Name=GOLS6; OrderedLocusNames=At4g26250; ORFNames=T25K17.60;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP GENE FAMILY.
RX PubMed=11846875; DOI=10.1046/j.0960-7412.2001.01227.x;
RA Taji T., Ohsumi C., Iuchi S., Seki M., Kasuga M., Kobayashi M.,
RA Yamaguchi-Shinozaki K., Shinozaki K.;
RT "Important roles of drought- and cold-inducible genes for galactinol
RT synthase in stress tolerance in Arabidopsis thaliana.";
RL Plant J. 29:417-426(2002).
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=18502973; DOI=10.1104/pp.108.122465;
RA Nishizawa A., Yabuta Y., Shigeoka S.;
RT "Galactinol and raffinose constitute a novel function to protect plants
RT from oxidative damage.";
RL Plant Physiol. 147:1251-1263(2008).
CC -!- FUNCTION: Galactinol synthase involved in the biosynthesis of raffinose
CC family oligosaccharides (RFOs) that function as osmoprotectants. May
CC promote plant stress tolerance (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=myo-inositol + UDP-alpha-D-galactose = alpha-D-galactosyl-
CC (1->3)-1D-myo-inositol + H(+) + UDP; Xref=Rhea:RHEA:12464,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:17268, ChEBI:CHEBI:17505,
CC ChEBI:CHEBI:58223, ChEBI:CHEBI:66914; EC=2.4.1.123;
CC -!- COFACTOR:
CC Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC Evidence={ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 8 family.
CC Galactosyltransferase subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB38954.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB79480.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL049171; CAB38954.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161564; CAB79480.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE85175.1; -; Genomic_DNA.
DR EMBL; AY142500; AAN13051.1; -; mRNA.
DR PIR; T06009; T06009.
DR RefSeq; NP_567741.2; NM_118758.3.
DR AlphaFoldDB; Q8H1S1; -.
DR SMR; Q8H1S1; -.
DR STRING; 3702.AT4G26250.1; -.
DR CAZy; GT8; Glycosyltransferase Family 8.
DR PaxDb; Q8H1S1; -.
DR PRIDE; Q8H1S1; -.
DR EnsemblPlants; AT4G26250.1; AT4G26250.1; AT4G26250.
DR GeneID; 828731; -.
DR Gramene; AT4G26250.1; AT4G26250.1; AT4G26250.
DR KEGG; ath:AT4G26250; -.
DR Araport; AT4G26250; -.
DR TAIR; locus:2136829; AT4G26250.
DR eggNOG; KOG1950; Eukaryota.
DR HOGENOM; CLU_049943_3_0_1; -.
DR InParanoid; Q8H1S1; -.
DR OMA; CHASTWE; -.
DR OrthoDB; 818680at2759; -.
DR PhylomeDB; Q8H1S1; -.
DR PRO; PR:Q8H1S1; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q8H1S1; baseline and differential.
DR Genevisible; Q8H1S1; AT.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016757; F:glycosyltransferase activity; IBA:GO_Central.
DR GO; GO:0047216; F:inositol 3-alpha-galactosyltransferase activity; ISS:UniProtKB.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006012; P:galactose metabolic process; ISS:UniProtKB.
DR GO; GO:0006950; P:response to stress; IEA:UniProt.
DR Gene3D; 3.90.550.10; -; 1.
DR InterPro; IPR002495; Glyco_trans_8.
DR InterPro; IPR030515; GOLS.
DR InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR PANTHER; PTHR11183:SF102; PTHR11183:SF102; 1.
DR Pfam; PF01501; Glyco_transf_8; 1.
DR SUPFAM; SSF53448; SSF53448; 1.
PE 2: Evidence at transcript level;
KW Carbohydrate metabolism; Cytoplasm; Galactose metabolism;
KW Glycosyltransferase; Manganese; Metal-binding; Reference proteome;
KW Transferase.
FT CHAIN 1..336
FT /note="Galactinol synthase 6"
FT /id="PRO_0000418662"
FT ACT_SITE 106
FT /evidence="ECO:0000250"
FT BINDING 122
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250"
FT BINDING 124
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250"
FT BINDING 260
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250"
SQ SEQUENCE 336 AA; 38851 MW; 63320151574E0900 CRC64;
MAQMSMTVEK SIKADVTVSH DRVKRAYVTF LAGNKDYWMG VVGLAKGLRK VKSAYPLVVA
ILPDVPEEHR QILLAQGCII REIEPVYPPE NKTGYSMAYY VINYSKLRIW EFVEYEKMIY
LDGDIQVFSN IDHLFDTPRG YLYAVKDCFC EISWSKTPQF KIGYCQQCPE KVTWPVESLG
SPPPVYFNAG MLVFEPNLLT YEDLLRVVQI TTPTYFAEQD FLNEYFTDIY KPIPSTYNLV
MAMLWRHPEH IDLDQISVIH YCANGSKPWR FDETEEHMDR EDIKMLVKKW WDIYEDSSLD
YKNFVETESK LSPINATLAS KESVGDVLIS LAPSAA
