GOLS7_ARATH
ID GOLS7_ARATH Reviewed; 332 AA.
AC Q4PSY4; O80766;
DT 05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT 19-JUL-2005, sequence version 1.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=Galactinol synthase 7;
DE Short=AtGolS7;
DE Short=GolS-7;
DE EC=2.4.1.123;
GN Name=GOLS7; OrderedLocusNames=At1g60450; ORFNames=T13D8.32;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W.,
RA Redman J.C., Wu H.C., Utterback T., Town C.D.;
RL Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP GENE FAMILY.
RX PubMed=11846875; DOI=10.1046/j.0960-7412.2001.01227.x;
RA Taji T., Ohsumi C., Iuchi S., Seki M., Kasuga M., Kobayashi M.,
RA Yamaguchi-Shinozaki K., Shinozaki K.;
RT "Important roles of drought- and cold-inducible genes for galactinol
RT synthase in stress tolerance in Arabidopsis thaliana.";
RL Plant J. 29:417-426(2002).
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=18502973; DOI=10.1104/pp.108.122465;
RA Nishizawa A., Yabuta Y., Shigeoka S.;
RT "Galactinol and raffinose constitute a novel function to protect plants
RT from oxidative damage.";
RL Plant Physiol. 147:1251-1263(2008).
CC -!- FUNCTION: Galactinol synthase involved in the biosynthesis of raffinose
CC family oligosaccharides (RFOs) that function as osmoprotectants. May
CC promote plant stress tolerance (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=myo-inositol + UDP-alpha-D-galactose = alpha-D-galactosyl-
CC (1->3)-1D-myo-inositol + H(+) + UDP; Xref=Rhea:RHEA:12464,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:17268, ChEBI:CHEBI:17505,
CC ChEBI:CHEBI:58223, ChEBI:CHEBI:66914; EC=2.4.1.123;
CC -!- COFACTOR:
CC Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC Evidence={ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 8 family.
CC Galactosyltransferase subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC24075.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC004473; AAC24075.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE33688.1; -; Genomic_DNA.
DR EMBL; DQ056502; AAY78659.1; -; mRNA.
DR PIR; T02295; T02295.
DR RefSeq; NP_176248.1; NM_104732.2.
DR AlphaFoldDB; Q4PSY4; -.
DR SMR; Q4PSY4; -.
DR STRING; 3702.AT1G60450.1; -.
DR CAZy; GT8; Glycosyltransferase Family 8.
DR PaxDb; Q4PSY4; -.
DR PRIDE; Q4PSY4; -.
DR ProteomicsDB; 247019; -.
DR EnsemblPlants; AT1G60450.1; AT1G60450.1; AT1G60450.
DR GeneID; 842340; -.
DR Gramene; AT1G60450.1; AT1G60450.1; AT1G60450.
DR KEGG; ath:AT1G60450; -.
DR Araport; AT1G60450; -.
DR TAIR; locus:2195668; AT1G60450.
DR eggNOG; KOG1950; Eukaryota.
DR HOGENOM; CLU_049943_3_0_1; -.
DR InParanoid; Q4PSY4; -.
DR OMA; EEPNMDR; -.
DR OrthoDB; 818680at2759; -.
DR PhylomeDB; Q4PSY4; -.
DR PRO; PR:Q4PSY4; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q4PSY4; baseline and differential.
DR Genevisible; Q4PSY4; AT.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016757; F:glycosyltransferase activity; IBA:GO_Central.
DR GO; GO:0047216; F:inositol 3-alpha-galactosyltransferase activity; ISS:UniProtKB.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006012; P:galactose metabolic process; ISS:UniProtKB.
DR Gene3D; 3.90.550.10; -; 1.
DR InterPro; IPR002495; Glyco_trans_8.
DR InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR Pfam; PF01501; Glyco_transf_8; 1.
DR SUPFAM; SSF53448; SSF53448; 1.
PE 2: Evidence at transcript level;
KW Carbohydrate metabolism; Cytoplasm; Galactose metabolism;
KW Glycosyltransferase; Manganese; Metal-binding; Reference proteome;
KW Transferase.
FT CHAIN 1..332
FT /note="Galactinol synthase 7"
FT /id="PRO_0000418663"
FT ACT_SITE 101
FT /evidence="ECO:0000250"
FT BINDING 117
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250"
FT BINDING 119
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250"
FT BINDING 255
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250"
SQ SEQUENCE 332 AA; 37768 MW; 224762F3C6984DC6 CRC64;
MTPETHVDMI NASEKAPKER AYVTFLAGNG DYVKGVVGLA KGLRKVKSAY PLVVAMLPDV
PEEHREILRS QGCIVREIEP VHPPDSQDAY ARAYYIINYS KLRIWNFEEY NKMIYLDADI
QVFGNIDDLF DMQDGYLHGV LSCFCEKIWS YTPLYSIGYC QYCPEKVVWP AEMESAPPSP
YFNAGMFVFE PNPLTYESLL QTLQVTPPTP FAEQDFLNMF FGKVFKPVSP VYNLILSVLW
RHPGKVDLES VKVVHYCPPG SKPWRYTGEE PNMDREDVKM LIKKWWDIYN DESLDFKPKS
PADLEATVLE STIIASVTEA PLSYSPAAPS AA
