GOL_DICDI
ID GOL_DICDI Reviewed; 579 AA.
AC O60950; Q54KK7;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 25-MAY-2022, entry version 89.
DE RecName: Full=Golvesin;
GN Name=gol; ORFNames=DDB_G0287271;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, AND TOPOLOGY.
RC STRAIN=AX2;
RX PubMed=11229601; DOI=10.1016/s0248-4900(00)01102-3;
RA Schneider N., Schwartz J.M., Koehler J., Becker M., Schwarz H., Gerisch G.;
RT "Golvesin-GFP fusions as distinct markers for Golgi and post-Golgi vesicles
RT in Dictyostelium cells.";
RL Biol. Cell 92:495-511(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3]
RP DISRUPTION PHENOTYPE.
RC STRAIN=AX4;
RX PubMed=10974109; DOI=10.1099/00221287-146-9-2219;
RA Li G., Alexander H., Schneider N., Alexander S.;
RT "Molecular basis for resistance to the anticancer drug cisplatin in
RT Dictyostelium.";
RL Microbiology 146:2219-2227(2000).
RN [4]
RP MUTAGENESIS OF 9-LEU--LEU-10, AND TARGETING SIGNALS WITHIN PROTEIN.
RC STRAIN=AX2;
RX PubMed=15511087; DOI=10.1078/0171-9335-00393;
RA Gerisch G., Benjak A., Koehler J., Weber I., Schneider N.;
RT "GFP-golvesin constructs to study Golgi tubulation and post-Golgi vesicle
RT dynamics in phagocytosis.";
RL Eur. J. Cell Biol. 83:297-303(2004).
CC -!- SUBCELLULAR LOCATION: Contractile vacuole membrane
CC {ECO:0000269|PubMed:11229601}; Single-pass type III membrane protein
CC {ECO:0000269|PubMed:11229601}. Endosome membrane
CC {ECO:0000305|PubMed:11229601}; Single-pass type III membrane protein
CC {ECO:0000305|PubMed:11229601}. Golgi apparatus membrane
CC {ECO:0000305|PubMed:11229601}; Single-pass type III membrane protein
CC {ECO:0000305|PubMed:11229601}.
CC -!- DISRUPTION PHENOTYPE: Cells are 5-fold more resistant to the antitumor
CC agent cisplatin than are wild-type cells.
CC {ECO:0000269|PubMed:10974109}.
CC -!- MISCELLANEOUS: The protein is normally retrieved from exocytic vesicles
CC before exocytosis occurs.
CC -!- MISCELLANEOUS: GFP-tagging of golvesin at its C-terminus traps it
CC within the Golgi apparatus, making this a useful marker for this
CC compartment.
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DR EMBL; U89350; AAC16756.1; -; Genomic_DNA.
DR EMBL; AAFI02000099; EAL63797.1; -; Genomic_DNA.
DR RefSeq; XP_637310.1; XM_632218.1.
DR AlphaFoldDB; O60950; -.
DR SMR; O60950; -.
DR STRING; 44689.DDB0191338; -.
DR PaxDb; O60950; -.
DR EnsemblProtists; EAL63797; EAL63797; DDB_G0287271.
DR GeneID; 8626047; -.
DR KEGG; ddi:DDB_G0287271; -.
DR dictyBase; DDB_G0287271; gol.
DR eggNOG; ENOG502SR14; Eukaryota.
DR HOGENOM; CLU_471290_0_0_1; -.
DR InParanoid; O60950; -.
DR OMA; MMNDMSS; -.
DR PRO; PR:O60950; -.
DR Proteomes; UP000002195; Chromosome 5.
DR GO; GO:0031164; C:contractile vacuolar membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0000331; C:contractile vacuole; IDA:dictyBase.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; TAS:dictyBase.
DR GO; GO:0010008; C:endosome membrane; IDA:dictyBase.
DR GO; GO:0005794; C:Golgi apparatus; IDA:dictyBase.
DR GO; GO:0030173; C:integral component of Golgi membrane; TAS:dictyBase.
DR GO; GO:0016021; C:integral component of membrane; TAS:dictyBase.
DR CDD; cd14488; CBM6-CBM35-CBM36_like_2; 1.
DR Gene3D; 3.40.710.10; -; 1.
DR InterPro; IPR012338; Beta-lactam/transpept-like.
DR InterPro; IPR033803; CBM6/CBM35/CBM36-like_2.
DR SUPFAM; SSF56601; SSF56601; 1.
PE 1: Evidence at protein level;
KW Endosome; Golgi apparatus; Membrane; Reference proteome; Signal-anchor;
KW Transmembrane; Transmembrane helix; Vacuole.
FT CHAIN 1..579
FT /note="Golvesin"
FT /id="PRO_0000328117"
FT TOPO_DOM 1..94
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 95..115
FT /note="Helical; Signal-anchor for type III membrane
FT protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 116..578
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 1..79
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1..75
FT /note="Required for targeting to the plasma membrane"
FT REGION 95..118
FT /note="Required for membrane targeting"
FT REGION 559..579
FT /note="Required for transfer to endosomes and contractile
FT vacuoles; the protein is trapped in the Golgi"
FT MUTAGEN 9..10
FT /note="LL->AA: Loss of endosomal retrieval; the protein is
FT targeted to the plasma membrane."
FT /evidence="ECO:0000269|PubMed:15511087"
SQ SEQUENCE 579 AA; 64175 MW; 37A9F1AEDF54F3ED CRC64;
MTSVNEHSLL INNNENNDNN NNHINQRNNK NNINNRNNIG VNNNNNNNNN NNNNNINNNN
NNNNNNNNNN NNNNNSNTGK IYLRKKKKWN FRKKILPMIV ILIITAIVVC LVVFSLPFDS
SNTIYNQSPF VIKEDDNLLE LAMKSRQNLL NIHGNQTISK LDFAILVSNP IGYWQLATFN
GKNNSNPQQT LFLPLLAAAV NWCMNQNNDP HCLQTAAGPM MNDMSSIHAG ILMDIITDAP
NQEFQTSNST GFQSWLTKRE SIELFLNSFN LLGNQTIINK FYPSNSGPTA TWGEGLIQKL
MGDNQMCPYD SALLMLNLVK SGILSEGQSY MTDLISRQTF STFTSIGFGL PPGTTLHSVL
GTSSSTKDLN EIAHLVLPNG GEMIFSIFSN GYENFGHAPY QSSILGNFAG DLIRSLGIDI
GCPNKIVMTS QDKNCTVTGA PWTLDTSIQA YNNSFLYISG GVTPTSTVTW SFTINVTGLY
EVCVWFPNGD LHTSVSYKVE PGDGMIYYFP VDQVHYGARW IRLDSFFIVA GNQPIISLSN
RGIDPSKTVV ADSVKLTRWP SSKGIPGFSN DFVIAESPE
