GOME_ACAGO
ID GOME_ACAGO Reviewed; 84 AA.
AC P82358; Q86RA2;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 13-APR-2004, sequence version 2.
DT 25-MAY-2022, entry version 70.
DE RecName: Full=Gomesin;
DE Flags: Precursor;
OS Acanthoscurria gomesiana (Tarantula spider) (Phormictopus pheopygus).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC Mygalomorphae; Theraphosidae; Acanthoscurria.
OX NCBI_TaxID=115339;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, PYROGLUTAMATE FORMATION AT
RP GLN-24, AND AMIDATION AT ARG-41.
RC TISSUE=Hemocyte;
RX PubMed=14505694; DOI=10.1016/s0965-1748(03)00115-2;
RA Lorenzini D.M., Fukuzawa A.H., da Silva P.I. Jr., Machado-Santelli G.,
RA Bijovsky A.T., Daffre S.;
RT "Molecular cloning, expression analysis and cellular localization of
RT gomesin, an anti-microbial peptide from hemocytes of the spider
RT Acanthoscurria gomesiana.";
RL Insect Biochem. Mol. Biol. 33:1011-1016(2003).
RN [2]
RP PROTEIN SEQUENCE OF 24-41, CHARACTERIZATION, MASS SPECTROMETRY, FUNCTION,
RP TISSUE SPECIFICITY, PYROGLUTAMATE FORMATION AT GLN-24, AMIDATION AT ARG-41,
RP AND DISULFIDE BONDS.
RC TISSUE=Hemocyte;
RX PubMed=10942757; DOI=10.1074/jbc.m001491200;
RA Silva P.I. Jr., Daffre S., Bulet P.;
RT "Isolation and characterization of gomesin, an 18-residue cysteine-rich
RT defense peptide from the spider Acanthoscurria gomesiana hemocytes with
RT sequence similarities to horseshoe crab antimicrobial peptides of the
RT tachyplesin family.";
RL J. Biol. Chem. 275:33464-33470(2000).
RN [3]
RP STRUCTURE BY NMR OF 24-41.
RX PubMed=11856345; DOI=10.1046/j.0014-2956.2002.02760.x;
RA Mandard N., Bulet P., Caille A., Daffre S., Vovelle F.;
RT "The solution structure of gomesin, an antimicrobial cysteine-rich peptide
RT from the spider.";
RL Eur. J. Biochem. 269:1190-1198(2002).
CC -!- FUNCTION: Active against several Gram-positive bacteria such as
CC Bacillus spp, Staphylococcus spp and E.faecalis, several Gram-negative
CC bacteria such as E.coli, K.pneumoniae, P.aeruginosa and Salmonella spp,
CC filamentous fungi such as N.crassa, T.viridae and yeasts such as
CC C.albicans. It is active against the parasite L.amazonensis as well. It
CC shows hemolytic activity. {ECO:0000269|PubMed:10942757}.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- TISSUE SPECIFICITY: In hemocytes only, but not in all hemocytes
CC observed. {ECO:0000269|PubMed:10942757, ECO:0000269|PubMed:14505694}.
CC -!- MASS SPECTROMETRY: Mass=2270.4; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:10942757};
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DR EMBL; AJ544540; CAD67587.1; -; mRNA.
DR PDB; 1KFP; NMR; -; A=24-41.
DR PDB; 6MY1; NMR; -; A=24-41.
DR PDB; 6MY2; NMR; -; A=24-41.
DR PDB; 6MY3; NMR; -; A=24-41.
DR PDBsum; 1KFP; -.
DR PDBsum; 6MY1; -.
DR PDBsum; 6MY2; -.
DR PDBsum; 6MY3; -.
DR AlphaFoldDB; P82358; -.
DR BMRB; P82358; -.
DR SMR; P82358; -.
DR TCDB; 1.C.34.3.1; the tachyplesin (tachyplesin) family.
DR EvolutionaryTrace; P82358; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR GO; GO:0044179; P:hemolysis in another organism; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW 3D-structure; Amidation; Antibiotic; Antimicrobial;
KW Cleavage on pair of basic residues; Cytolysis; Direct protein sequencing;
KW Disulfide bond; Fungicide; Hemolysis; Pyrrolidone carboxylic acid;
KW Secreted; Signal.
FT SIGNAL 1..23
FT /evidence="ECO:0000269|PubMed:10942757"
FT PEPTIDE 24..41
FT /note="Gomesin"
FT /id="PRO_0000021338"
FT PROPEP 42..84
FT /id="PRO_0000021339"
FT MOD_RES 24
FT /note="Pyrrolidone carboxylic acid"
FT /evidence="ECO:0000269|PubMed:10942757,
FT ECO:0000269|PubMed:14505694"
FT MOD_RES 41
FT /note="Arginine amide"
FT /evidence="ECO:0000269|PubMed:10942757,
FT ECO:0000269|PubMed:14505694"
FT DISULFID 25..38
FT /evidence="ECO:0000269|PubMed:10942757"
FT DISULFID 29..34
FT /evidence="ECO:0000269|PubMed:10942757"
FT STRAND 25..30
FT /evidence="ECO:0007829|PDB:1KFP"
FT STRAND 33..38
FT /evidence="ECO:0007829|PDB:1KFP"
SQ SEQUENCE 84 AA; 9688 MW; 2B346C21A41F516E CRC64;
MNRTRLFACL LLAVLILVHE SNAQCRRLCY KQRCVTYCRG RGKRSLDETN VGTSDVEKRA
FDDSNVPSLV EERELEDEGS FIFD
