ID   GON2_LITCT              Reviewed;          93 AA.
AC   Q9DG36; Q90Y64;
DT   11-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   25-MAY-2022, entry version 58.
DE   RecName: Full=Progonadoliberin-2;
DE   AltName: Full=Progonadoliberin II;
DE   Contains:
DE     RecName: Full=Gonadoliberin-2;
DE     AltName: Full=Gonadoliberin II;
DE     AltName: Full=Gonadotropin-releasing hormone II;
DE              Short=GnRH II;
DE     AltName: Full=LHRH II;
DE     AltName: Full=Luliberin II;
DE     AltName: Full=Luteinizing hormone-releasing hormone II;
DE   Contains:
DE     RecName: Full=GnRH-associated peptide 2;
DE     AltName: Full=GAP2;
DE     AltName: Full=GnRH-associated peptide II;
DE   Flags: Precursor;
GN   Name=gnrh2;
OS   Lithobates catesbeianus (American bullfrog) (Rana catesbeiana).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Ranoidea; Ranidae; Lithobates.
OX   NCBI_TaxID=8400 {ECO:0000312|EMBL:AAG21894.1};
RN   [1] {ECO:0000305}
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY, AND
RP   DEVELOPMENTAL STAGE.
RC   TISSUE=Hindbrain, and Midbrain;
RX   PubMed=11170016;
RX   DOI=10.1002/1097-010x(20010215)289:3<190::aid-jez6>3.0.co;2-e;
RA   Wang L., Yoo M.S., Kang H.M., Im W.B., Choi H.S., Bogerd J., Kwon H.B.;
RT   "Cloning and characterization of cDNAs encoding the GnRH1 and GnRH2
RT   precursors from bullfrog (Rana catesbeiana).";
RL   J. Exp. Zool. 289:190-201(2001).
CC   -!- FUNCTION: Stimulates the secretion of gonadotropins.
CC       {ECO:0000255|RuleBase:RU000635, ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:P51925}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1 {ECO:0000269|PubMed:11170016};
CC         IsoId=Q9DG36-1; Sequence=Displayed;
CC       Name=2 {ECO:0000269|PubMed:11170016};
CC         IsoId=Q9DG36-2; Sequence=VSP_050476;
CC   -!- TISSUE SPECIFICITY: Midbrain and hindbrain.
CC       {ECO:0000269|PubMed:11170016}.
CC   -!- DEVELOPMENTAL STAGE: Expressed at significantly higher levels during
CC       hibernation and post-breeding. Not expressed in pituitary.
CC       {ECO:0000269|PubMed:11170016}.
CC   -!- SIMILARITY: Belongs to the GnRH family. {ECO:0000305}.
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DR   EMBL; AF192464; AAG21894.1; -; mRNA.
DR   EMBL; AF186096; AAL05971.1; -; mRNA.
DR   AlphaFoldDB; Q9DG36; -.
DR   SMR; Q9DG36; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005183; F:gonadotropin hormone-releasing hormone activity; NAS:UniProtKB.
DR   GO; GO:0009755; P:hormone-mediated signaling pathway; NAS:UniProtKB.
DR   GO; GO:0000003; P:reproduction; NAS:UniProtKB.
DR   InterPro; IPR002012; GnRH.
DR   InterPro; IPR019792; Gonadoliberin.
DR   PANTHER; PTHR10522; PTHR10522; 1.
DR   Pfam; PF00446; GnRH; 1.
DR   PROSITE; PS00473; GNRH; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Amidation; Cleavage on pair of basic residues;
KW   Hormone; Pyrrolidone carboxylic acid; Secreted; Signal.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   CHAIN           25..93
FT                   /note="Progonadoliberin-2"
FT                   /id="PRO_0000012547"
FT   PEPTIDE         25..34
FT                   /note="Gonadoliberin-2"
FT                   /id="PRO_0000012548"
FT   PEPTIDE         38..93
FT                   /note="GnRH-associated peptide 2"
FT                   /id="PRO_0000012549"
FT   MOD_RES         25
FT                   /note="Pyrrolidone carboxylic acid"
FT                   /evidence="ECO:0000250"
FT   MOD_RES         34
FT                   /note="Glycine amide"
FT                   /evidence="ECO:0000250"
FT   VAR_SEQ         75..82
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:11170016"
FT                   /id="VSP_050476"
SQ   SEQUENCE   93 AA;  10668 MW;  B3DE9920DCF6EAA9 CRC64;
     MACQRHLLFL LLVLFAVSTQ LSHGQHWSHG WYPGGKRELD MPASPEVSEE IKLCEGEECA
     YLRNPRKNLL KNILGRSNQK KNADVLARQL QKK