GOS12_ARATH
ID GOS12_ARATH Reviewed; 257 AA.
AC O22151; F4IW37; Q2HIF8;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 22-FEB-2012, sequence version 2.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Golgi SNAP receptor complex member 1-2;
DE AltName: Full=Golgi SNARE 12 protein;
DE Short=AtGOS12;
GN Name=GOS12; OrderedLocusNames=At2g45200; ORFNames=F4L23.29;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RA Sanderfoot A.A., Raikhel N.V.;
RT "Arabidopsis Golgi SNAREs.";
RL Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-51 (ISOFORM 2), AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Root;
RX PubMed=18433157; DOI=10.1021/pr8000173;
RA de la Fuente van Bentem S., Anrather D., Dohnal I., Roitinger E.,
RA Csaszar E., Joore J., Buijnink J., Carreri A., Forzani C., Lorkovic Z.J.,
RA Barta A., Lecourieux D., Verhounig A., Jonak C., Hirt H.;
RT "Site-specific phosphorylation profiling of Arabidopsis proteins by mass
RT spectrometry and peptide chip analysis.";
RL J. Proteome Res. 7:2458-2470(2008).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=cv. Columbia;
RX PubMed=19245862; DOI=10.1016/j.jprot.2009.02.004;
RA Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E.,
RA Rathjen J.P., Peck S.C.;
RT "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis
RT thaliana.";
RL J. Proteomics 72:439-451(2009).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
CC -!- FUNCTION: Involved in transport from the ER to the Golgi apparatus as
CC well as in intra-Golgi transport. It belongs to a super-family of
CC proteins called t-SNAREs or soluble NSF (N-ethylmaleimide-sensitive
CC factor) attachment protein receptor (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of several multiprotein Golgi SNARE complexes.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000250}; Single-
CC pass type IV membrane protein {ECO:0000250}. Endoplasmic reticulum
CC membrane {ECO:0000250}; Single-pass type IV membrane protein
CC {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=O22151-2; Sequence=Displayed;
CC Name=2;
CC IsoId=O22151-1; Sequence=VSP_042317;
CC -!- SIMILARITY: Belongs to the GOSR1 family. {ECO:0000305}.
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DR EMBL; AF357529; AAK48905.1; -; mRNA.
DR EMBL; AC002387; AAB82642.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC10524.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC10525.1; -; Genomic_DNA.
DR EMBL; BT024623; ABD43021.1; -; mRNA.
DR PIR; F84887; F84887.
DR RefSeq; NP_001189748.1; NM_001202819.1. [O22151-2]
DR RefSeq; NP_182045.1; NM_130083.5. [O22151-1]
DR AlphaFoldDB; O22151; -.
DR SMR; O22151; -.
DR BioGRID; 4464; 8.
DR IntAct; O22151; 7.
DR STRING; 3702.AT2G45200.2; -.
DR iPTMnet; O22151; -.
DR PaxDb; O22151; -.
DR PRIDE; O22151; -.
DR ProteomicsDB; 248448; -. [O22151-2]
DR EnsemblPlants; AT2G45200.1; AT2G45200.1; AT2G45200. [O22151-1]
DR EnsemblPlants; AT2G45200.2; AT2G45200.2; AT2G45200. [O22151-2]
DR GeneID; 819128; -.
DR Gramene; AT2G45200.1; AT2G45200.1; AT2G45200. [O22151-1]
DR Gramene; AT2G45200.2; AT2G45200.2; AT2G45200. [O22151-2]
DR KEGG; ath:AT2G45200; -.
DR Araport; AT2G45200; -.
DR TAIR; locus:2050847; AT2G45200.
DR eggNOG; KOG3208; Eukaryota.
DR HOGENOM; CLU_078034_2_1_1; -.
DR InParanoid; O22151; -.
DR OMA; QAYAVND; -.
DR OrthoDB; 1319902at2759; -.
DR PRO; PR:O22151; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O22151; baseline and differential.
DR Genevisible; O22151; AT.
DR GO; GO:0005801; C:cis-Golgi network; IBA:GO_Central.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005768; C:endosome; HDA:TAIR.
DR GO; GO:0005794; C:Golgi apparatus; HDA:TAIR.
DR GO; GO:0005797; C:Golgi medial cisterna; IBA:GO_Central.
DR GO; GO:0000139; C:Golgi membrane; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0000325; C:plant-type vacuole; HDA:TAIR.
DR GO; GO:0031201; C:SNARE complex; IBA:GO_Central.
DR GO; GO:0005802; C:trans-Golgi network; HDA:TAIR.
DR GO; GO:0005484; F:SNAP receptor activity; IBA:GO_Central.
DR GO; GO:0006888; P:endoplasmic reticulum to Golgi vesicle-mediated transport; IBA:GO_Central.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0006906; P:vesicle fusion; IBA:GO_Central.
DR InterPro; IPR023601; Golgi_SNAP_su1.
DR PANTHER; PTHR21094; PTHR21094; 1.
DR PIRSF; PIRSF027109; Golgi_SNARE; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Endoplasmic reticulum;
KW ER-Golgi transport; Golgi apparatus; Membrane; Phosphoprotein;
KW Protein transport; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..257
FT /note="Golgi SNAP receptor complex member 1-2"
FT /id="PRO_0000212548"
FT TOPO_DOM 1..235
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 236..256
FT /note="Helical; Anchor for type IV membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 257
FT /note="Vesicular"
FT /evidence="ECO:0000255"
FT COILED 113..147
FT /evidence="ECO:0000255"
FT VAR_SEQ 45..62
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.1, ECO:0000303|Ref.4"
FT /id="VSP_042317"
FT MOD_RES O22151-1:51
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18433157"
SQ SEQUENCE 257 AA; 28522 MW; 30B64A7613F16BBE CRC64;
MTESSLDLQE SGWEELRREA RKIEGDLDVK LSSYAKLGAR FTQGDTDLVM NYEKVLKCVL
VSGYVDTGSP TVGSGRSWKS MEMEIQSLLE KLLDINDSMS RCAASAAPTT SVTQKLARHR
DILHEYTQEF RRIKGNINSL REHAELLSSV RDDISEYKAS GSMSPGVQVL RERASIHGSI
SHIDDVIGQA QATRAVLGSQ RSLFSDVQGK VKNLGDKFPV IRGLLGSIKR KRSRDTLILS
AVIAACTLFL IIYWLSK
