GOSR2_DROME
ID GOSR2_DROME Reviewed; 216 AA.
AC Q9VRL2; B5RJH1; Q8MRU9;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Probable Golgi SNAP receptor complex member 2;
GN Name=Membrin {ECO:0000312|FlyBase:FBgn0260856};
GN ORFNames=CG4780 {ECO:0000312|FlyBase:FBgn0260856};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley;
RA Carlson J.W., Booth B., Frise E., Park S., Wan K.H., Yu C., Celniker S.E.;
RL Submitted (SEP-2008) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP IDENTIFICATION.
RX PubMed=10908590; DOI=10.1083/jcb.150.2.f77;
RA Littleton J.T.;
RT "A genomic analysis of membrane trafficking and neurotransmitter release in
RT Drosophila.";
RL J. Cell Biol. 150:F77-F82(2000).
CC -!- FUNCTION: Involved in transport of proteins from the cis/medial-Golgi
CC to the trans-Golgi network. {ECO:0000250|UniProtKB:O35165}.
CC -!- SUBUNIT: Part of a unique SNARE complex.
CC {ECO:0000250|UniProtKB:O35165}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus, cis-Golgi network membrane
CC {ECO:0000250|UniProtKB:O35165}; Single-pass type IV membrane protein
CC {ECO:0000255}. Golgi apparatus membrane {ECO:0000250|UniProtKB:O35165}.
CC Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:O35165}.
CC Note=Concentrated most in the intermediate compartment/cis-Golgi
CC network and the cis-Golgi cisternae 1 and 2. Greatly reduced in
CC concentration at the trans end of the Golgi apparatus.
CC {ECO:0000250|UniProtKB:O35165}.
CC -!- SIMILARITY: Belongs to the GOSR2 family. {ECO:0000305}.
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DR EMBL; AE014296; AAF50783.1; -; Genomic_DNA.
DR EMBL; AY119259; AAM51119.1; -; mRNA.
DR EMBL; BT044445; ACH92510.1; -; mRNA.
DR RefSeq; NP_647960.1; NM_139703.4.
DR AlphaFoldDB; Q9VRL2; -.
DR SMR; Q9VRL2; -.
DR BioGRID; 64080; 28.
DR DIP; DIP-21257N; -.
DR IntAct; Q9VRL2; 14.
DR STRING; 7227.FBpp0076849; -.
DR PaxDb; Q9VRL2; -.
DR PRIDE; Q9VRL2; -.
DR DNASU; 38614; -.
DR EnsemblMetazoa; FBtr0077145; FBpp0076849; FBgn0260856.
DR GeneID; 38614; -.
DR KEGG; dme:Dmel_CG4780; -.
DR UCSC; CG4780-RA; d. melanogaster.
DR CTD; 38614; -.
DR FlyBase; FBgn0260856; Membrin.
DR VEuPathDB; VectorBase:FBgn0260856; -.
DR eggNOG; KOG3251; Eukaryota.
DR GeneTree; ENSGT00940000164674; -.
DR HOGENOM; CLU_083740_0_1_1; -.
DR InParanoid; Q9VRL2; -.
DR OMA; RMRVDQL; -.
DR OrthoDB; 1139640at2759; -.
DR PhylomeDB; Q9VRL2; -.
DR Reactome; R-DME-204005; COPII-mediated vesicle transport.
DR Reactome; R-DME-6807878; COPI-mediated anterograde transport.
DR Reactome; R-DME-6811438; Intra-Golgi traffic.
DR BioGRID-ORCS; 38614; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 38614; -.
DR PRO; PR:Q9VRL2; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0260856; Expressed in saliva-secreting gland and 24 other tissues.
DR Genevisible; Q9VRL2; DM.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IBA:GO_Central.
DR GO; GO:0012507; C:ER to Golgi transport vesicle membrane; IBA:GO_Central.
DR GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0031902; C:late endosome membrane; IBA:GO_Central.
DR GO; GO:0031201; C:SNARE complex; ISS:FlyBase.
DR GO; GO:0005484; F:SNAP receptor activity; ISS:FlyBase.
DR GO; GO:0000149; F:SNARE binding; IBA:GO_Central.
DR GO; GO:0035149; P:lumen formation, open tracheal system; HMP:FlyBase.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0006906; P:vesicle fusion; ISS:FlyBase.
DR GO; GO:0016192; P:vesicle-mediated transport; ISS:FlyBase.
DR Gene3D; 1.20.58.400; -; 1.
DR InterPro; IPR027027; GOSR2/Membrin/Bos1.
DR InterPro; IPR038407; v-SNARE_N_sf.
DR PIRSF; PIRSF028865; Membrin-2; 1.
PE 2: Evidence at transcript level;
KW Coiled coil; Endoplasmic reticulum; Golgi apparatus; Membrane;
KW Protein transport; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..216
FT /note="Probable Golgi SNAP receptor complex member 2"
FT /id="PRO_0000212553"
FT TOPO_DOM 1..194
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 195..215
FT /note="Helical; Anchor for type IV membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 216
FT /note="Vesicular"
FT /evidence="ECO:0000255"
FT COILED 62..103
FT /evidence="ECO:0000255"
FT CONFLICT 125
FT /note="Y -> H (in Ref. 3; AAM51119)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 216 AA; 24833 MW; 3CD23EA81BA9851F CRC64;
MESLYHQTNN VVKDIERDFQ RLSQLSAQES LDVENGIQLK ITQANANCDR LDVLLYKVPP
SQRQSSKLRV DQLKYDLRHL QTSLQTARER RQRRMQEISE REQLLNHRFT ANSAQPEETR
LQLDYELQHH TQLGNAHRGV DDMIASGSGI LESLISQRMT LGGAHKRIQA IGSTLGLSNH
TMKLIERRLV EDRRIFIGGV VVTLLIIALI IYFLVL
