GOSR2_MOUSE
ID GOSR2_MOUSE Reviewed; 212 AA.
AC O35166; Q3UDN0; Q9CR77;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 31-AUG-2004, sequence version 2.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=Golgi SNAP receptor complex member 2;
DE AltName: Full=27 kDa Golgi SNARE protein;
DE AltName: Full=Membrin;
GN Name=Gosr2; Synonyms=Gs27;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=9349823; DOI=10.1038/39923;
RA Lowe S.L., Peter F., Subramaniam V.N., Wong S.H., Hong W.;
RT "A SNARE involved in protein transport through the Golgi apparatus.";
RL Nature 389:881-884(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Bone marrow, Embryo, and Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N, and FVB/N-3; TISSUE=Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Heart, Kidney, Lung, Pancreas, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Involved in transport of proteins from the cis/medial-Golgi
CC to the trans-Golgi network. {ECO:0000250|UniProtKB:O35165}.
CC -!- SUBUNIT: Part of a unique SNARE complex composed of the Golgi SNAREs
CC GOSR1, STX5 and YKT6. {ECO:0000250|UniProtKB:O35165}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus, cis-Golgi network membrane
CC {ECO:0000250|UniProtKB:O35165}; Single-pass type IV membrane protein
CC {ECO:0000255}. Golgi apparatus membrane {ECO:0000250|UniProtKB:O35165}.
CC Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:O35165}.
CC Note=Concentrated most in the intermediate compartment/cis-Golgi
CC network and the cis-Golgi cisternae 1 and 2. Greatly reduced in
CC concentration at the trans end of the Golgi apparatus.
CC {ECO:0000250|UniProtKB:O35165}.
CC -!- SIMILARITY: Belongs to the GOSR2 family. {ECO:0000305}.
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DR EMBL; AF007550; AAB82653.1; -; mRNA.
DR EMBL; AK005697; BAB24194.1; -; mRNA.
DR EMBL; AK013052; BAB28622.1; -; mRNA.
DR EMBL; AK150004; BAE29231.1; -; mRNA.
DR EMBL; BC008525; AAH08525.1; -; mRNA.
DR EMBL; BC051253; AAH51253.1; -; mRNA.
DR CCDS; CCDS25521.1; -.
DR RefSeq; NP_062624.2; NM_019650.3.
DR AlphaFoldDB; O35166; -.
DR SMR; O35166; -.
DR BioGRID; 208018; 2.
DR STRING; 10090.ENSMUSP00000021329; -.
DR PhosphoSitePlus; O35166; -.
DR EPD; O35166; -.
DR MaxQB; O35166; -.
DR PaxDb; O35166; -.
DR PRIDE; O35166; -.
DR ProteomicsDB; 271132; -.
DR DNASU; 56494; -.
DR Ensembl; ENSMUST00000021329; ENSMUSP00000021329; ENSMUSG00000020946.
DR GeneID; 56494; -.
DR KEGG; mmu:56494; -.
DR UCSC; uc007lvo.2; mouse.
DR CTD; 9570; -.
DR MGI; MGI:1927204; Gosr2.
DR VEuPathDB; HostDB:ENSMUSG00000020946; -.
DR eggNOG; KOG3251; Eukaryota.
DR GeneTree; ENSGT00950000183192; -.
DR InParanoid; O35166; -.
DR OMA; RMRVDQL; -.
DR OrthoDB; 1139640at2759; -.
DR PhylomeDB; O35166; -.
DR TreeFam; TF313702; -.
DR Reactome; R-MMU-204005; COPII-mediated vesicle transport.
DR Reactome; R-MMU-5694530; Cargo concentration in the ER.
DR Reactome; R-MMU-6807878; COPI-mediated anterograde transport.
DR Reactome; R-MMU-6811438; Intra-Golgi traffic.
DR BioGRID-ORCS; 56494; 20 hits in 74 CRISPR screens.
DR ChiTaRS; Gosr2; mouse.
DR PRO; PR:O35166; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; O35166; protein.
DR Bgee; ENSMUSG00000020946; Expressed in metanephric ureteric bud and 259 other tissues.
DR ExpressionAtlas; O35166; baseline and differential.
DR Genevisible; O35166; MM.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IDA:MGI.
DR GO; GO:0012507; C:ER to Golgi transport vesicle membrane; IBA:GO_Central.
DR GO; GO:0005794; C:Golgi apparatus; ISO:MGI.
DR GO; GO:0000139; C:Golgi membrane; IDA:MGI.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0031902; C:late endosome membrane; IBA:GO_Central.
DR GO; GO:0031201; C:SNARE complex; ISS:UniProtKB.
DR GO; GO:0005484; F:SNAP receptor activity; IBA:GO_Central.
DR GO; GO:0000149; F:SNARE binding; IBA:GO_Central.
DR GO; GO:0006891; P:intra-Golgi vesicle-mediated transport; ISS:UniProtKB.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0006906; P:vesicle fusion; IBA:GO_Central.
DR GO; GO:0016192; P:vesicle-mediated transport; IDA:MGI.
DR InterPro; IPR027027; GOSR2/Membrin/Bos1.
DR PIRSF; PIRSF028865; Membrin-2; 1.
PE 1: Evidence at protein level;
KW Acetylation; Coiled coil; Endoplasmic reticulum; Golgi apparatus; Membrane;
KW Protein transport; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..212
FT /note="Golgi SNAP receptor complex member 2"
FT /id="PRO_0000212550"
FT TOPO_DOM 1..190
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 191..211
FT /note="Helical; Anchor for type IV membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 212
FT /note="Vesicular"
FT /evidence="ECO:0000255"
FT COILED 60..92
FT /evidence="ECO:0000255"
FT MOTIF 118..120
FT /note="IxM motif; signal for cargo packaging into COPII-
FT coated vesicles"
FT /evidence="ECO:0000250|UniProtKB:O14653"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000250|UniProtKB:O14653"
FT CONFLICT 99
FT /note="R -> I (in Ref. 1; AAB82653)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 212 AA; 24725 MW; 1622371B06090A6C CRC64;
MEPLYQQTNK QVQEIQSHMG RLERADKQSV HLVENEIQAS IEQIFSHLER LEILSSKEPL
NRRQNAKLRV DQLKYDVQHL QTALRNFQHR RQVREQQERQ RDELLSRTFT TNDSDTTIPM
DESLQFNSSL HNIHHGMDDL IGGGHSILEG LRAQRLTLKG TQKKILDIAN MLGLSNTVMR
LIEKRAFQDK YFMIGGMLLT CAVMFLVVQY LT
