GOSR2_RAT
ID GOSR2_RAT Reviewed; 212 AA.
AC O35165; O08566; Q6GSW2;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2007, sequence version 2.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Golgi SNAP receptor complex member 2;
DE AltName: Full=27 kDa Golgi SNARE protein;
DE AltName: Full=Membrin;
GN Name=Gosr2; Synonyms=Gs27;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND SUBUNIT.
RC TISSUE=Brain;
RX PubMed=9349823; DOI=10.1038/39923;
RA Lowe S.L., Peter F., Subramaniam V.N., Wong S.H., Hong W.;
RT "A SNARE involved in protein transport through the Golgi apparatus.";
RL Nature 389:881-884(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 11-17; 100-107 AND 156-159,
RP FUNCTION, SUBUNIT, AND SUBCELLULAR LOCATION.
RC STRAIN=Sprague-Dawley; TISSUE=Liver;
RX PubMed=9094723; DOI=10.1016/s0092-8674(00)80191-9;
RA Hay J.C., Chao D.S., Kuo C.S., Scheller R.H.;
RT "Protein interactions regulating vesicle transport between the endoplasmic
RT reticulum and Golgi apparatus in mammalian cells.";
RL Cell 89:149-158(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Prostate;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP INTERACTION WITH GOSR1; STX5 AND YKT6.
RX PubMed=11323436; DOI=10.1074/jbc.m102786200;
RA Zhang T., Hong W.;
RT "Ykt6 forms a SNARE complex with syntaxin 5, GS28, and Bet1 and
RT participates in a late stage in endoplasmic reticulum-Golgi transport.";
RL J. Biol. Chem. 276:27480-27487(2001).
RN [5]
RP SUBCELLULAR LOCATION.
RX PubMed=14742712; DOI=10.1091/mbc.e03-08-0625;
RA Volchuk A., Ravazzola M., Perrelet A., Eng W.S., Di Liberto M.,
RA Varlamov O., Fukasawa M., Engel T., Sollner T.H., Rothman J.E., Orci L.;
RT "Countercurrent distribution of two distinct SNARE complexes mediating
RT transport within the Golgi stack.";
RL Mol. Biol. Cell 15:1506-1518(2004).
CC -!- FUNCTION: Involved in transport of proteins from the cis/medial-Golgi
CC to the trans-Golgi network. {ECO:0000269|PubMed:9094723}.
CC -!- SUBUNIT: Part of a unique SNARE complex composed of the Golgi SNAREs
CC GOSR1, STX5 and YKT6. {ECO:0000269|PubMed:11323436,
CC ECO:0000269|PubMed:9094723, ECO:0000269|PubMed:9349823}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus, cis-Golgi network membrane
CC {ECO:0000269|PubMed:14742712}; Single-pass type IV membrane protein
CC {ECO:0000255}. Golgi apparatus membrane {ECO:0000269|PubMed:14742712}.
CC Endoplasmic reticulum membrane {ECO:0000269|PubMed:9094723}.
CC Note=Concentrated most in the intermediate compartment/cis-Golgi
CC network and the cis-Golgi cisternae 1 and 2. Greatly reduced in
CC concentration at the trans end of the Golgi apparatus.
CC {ECO:0000269|PubMed:14742712}.
CC -!- SIMILARITY: Belongs to the GOSR2 family. {ECO:0000305}.
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DR EMBL; AF007549; AAB82652.1; -; mRNA.
DR EMBL; U91539; AAC53131.1; -; mRNA.
DR EMBL; BC061994; AAH61994.1; -; mRNA.
DR RefSeq; NP_113873.1; NM_031685.2.
DR AlphaFoldDB; O35165; -.
DR SMR; O35165; -.
DR CORUM; O35165; -.
DR IntAct; O35165; 4.
DR STRING; 10116.ENSRNOP00000005046; -.
DR jPOST; O35165; -.
DR PaxDb; O35165; -.
DR PRIDE; O35165; -.
DR Ensembl; ENSRNOT00000114651; ENSRNOP00000084049; ENSRNOG00000003506.
DR GeneID; 64154; -.
DR KEGG; rno:64154; -.
DR CTD; 9570; -.
DR RGD; 62079; Gosr2.
DR eggNOG; KOG3251; Eukaryota.
DR GeneTree; ENSGT00950000183192; -.
DR HOGENOM; CLU_083740_0_1_1; -.
DR InParanoid; O35165; -.
DR OMA; RMRVDQL; -.
DR OrthoDB; 1139640at2759; -.
DR PhylomeDB; O35165; -.
DR TreeFam; TF313702; -.
DR Reactome; R-RNO-204005; COPII-mediated vesicle transport.
DR Reactome; R-RNO-5694530; Cargo concentration in the ER.
DR Reactome; R-RNO-6807878; COPI-mediated anterograde transport.
DR Reactome; R-RNO-6811438; Intra-Golgi traffic.
DR PRO; PR:O35165; -.
DR Proteomes; UP000002494; Chromosome 10.
DR Bgee; ENSRNOG00000003506; Expressed in adult mammalian kidney and 19 other tissues.
DR Genevisible; O35165; RN.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; ISO:RGD.
DR GO; GO:0012507; C:ER to Golgi transport vesicle membrane; IBA:GO_Central.
DR GO; GO:0005794; C:Golgi apparatus; ISO:RGD.
DR GO; GO:0000139; C:Golgi membrane; ISS:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0031902; C:late endosome membrane; IBA:GO_Central.
DR GO; GO:0031201; C:SNARE complex; IDA:UniProtKB.
DR GO; GO:0005484; F:SNAP receptor activity; IBA:GO_Central.
DR GO; GO:0000149; F:SNARE binding; IBA:GO_Central.
DR GO; GO:0006891; P:intra-Golgi vesicle-mediated transport; ISS:UniProtKB.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0006906; P:vesicle fusion; IBA:GO_Central.
DR GO; GO:0016192; P:vesicle-mediated transport; ISO:RGD.
DR Gene3D; 1.20.58.400; -; 1.
DR InterPro; IPR027027; GOSR2/Membrin/Bos1.
DR InterPro; IPR038407; v-SNARE_N_sf.
DR PIRSF; PIRSF028865; Membrin-2; 1.
PE 1: Evidence at protein level;
KW Acetylation; Coiled coil; Direct protein sequencing; Endoplasmic reticulum;
KW Golgi apparatus; Membrane; Protein transport; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..212
FT /note="Golgi SNAP receptor complex member 2"
FT /id="PRO_0000212551"
FT TOPO_DOM 1..190
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 191..211
FT /note="Helical; Anchor for type IV membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 212
FT /note="Vesicular"
FT /evidence="ECO:0000255"
FT COILED 61..107
FT /evidence="ECO:0000255"
FT MOTIF 118..120
FT /note="IxM motif; signal for cargo packaging into COPII-
FT coated vesicles"
FT /evidence="ECO:0000250|UniProtKB:O14653"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000250|UniProtKB:O14653"
FT CONFLICT 114
FT /note="S -> Y (in Ref. 1; AAB82652)"
FT /evidence="ECO:0000305"
FT CONFLICT 187..188
FT /note="FQ -> LE (in Ref. 1; AAB82652)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 212 AA; 24607 MW; 164380B16A4532A0 CRC64;
MEPLYQQTHK QVHEIQSHMG RLETADKQSV HLVENEIQAS IDQIFSHLER LEILSSKEPP
NRRQNAKLRV DQLKYDVQHL QTALRNFQHR RQAKEQQERQ RDELLSRTFT TNDSDTTIPM
DESLQFNSSL QNIHHGMDDL IGGGHSILEG LRAQRLTLKG TQKKILDIAN MLGLSNTVMR
LIEKRAFQDK YFMIGGMLLT CAVMFLVVQY LT
