GOT1B_HUMAN
ID GOT1B_HUMAN Reviewed; 138 AA.
AC Q9Y3E0; B2R4R4; Q54A40; Q6I9W6; Q9P1R9;
DT 10-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 161.
DE RecName: Full=Vesicle transport protein GOT1B;
DE AltName: Full=Germ cell tumor 2;
DE AltName: Full=Golgi transport 1 homolog B;
DE AltName: Full=Putative NF-kappa-B-activating protein 470;
DE AltName: Full=hGOT1a;
GN Name=GOLT1B; Synonyms=GCT2, GOT1A;
GN ORFNames=CGI-141, HDCMA39P, UNQ432/PRO793;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=10810093; DOI=10.1101/gr.10.5.703;
RA Lai C.-H., Chou C.-Y., Ch'ang L.-Y., Liu C.-S., Lin W.-C.;
RT "Identification of novel human genes evolutionarily conserved in
RT Caenorhabditis elegans by comparative proteomics.";
RL Genome Res. 10:703-713(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Amygdala;
RX PubMed=11230166; DOI=10.1101/gr.gr1547r;
RA Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S.,
RA Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J.,
RA Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W.,
RA Ottenwaelder B., Obermaier B., Tampe J., Heubner D., Wambutt R., Korn B.,
RA Klein M., Poustka A.;
RT "Towards a catalog of human genes and proteins: sequencing and analysis of
RT 500 novel complete protein coding human cDNAs.";
RL Genome Res. 11:422-435(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Lung fibroblast;
RX PubMed=12761501; DOI=10.1038/sj.onc.1206406;
RA Matsuda A., Suzuki Y., Honda G., Muramatsu S., Matsuzaki O., Nagano Y.,
RA Doi T., Shimotohno K., Harada T., Nishida E., Hayashi H., Sugano S.;
RT "Large-scale identification and characterization of human genes that
RT activate NF-kappaB and MAPK signaling pathways.";
RL Oncogene 22:3307-3318(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=12975309; DOI=10.1101/gr.1293003;
RA Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S., Huang A.,
RA Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D.,
RA Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L.,
RA Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C.,
RA Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J.,
RA Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.;
RT "The secreted protein discovery initiative (SPDI), a large-scale effort to
RT identify novel human secreted and transmembrane proteins: a bioinformatics
RT assessment.";
RL Genome Res. 13:2265-2270(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Dendritic cell;
RA Zhao Z., Huang X., Li N., Zhu X., Cao X.;
RT "A novel gene from human dendritic cell.";
RL Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [9]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Ovary;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [10]
RP PUTATIVE FUNCTION, SUBCELLULAR LOCATION, AND TOPOLOGY.
RX PubMed=10406798; DOI=10.1093/emboj/18.14.3934;
RA Conchon S., Cao X., Barlowe C., Pelham H.R.;
RT "Got1p and Sft2p: membrane proteins involved in traffic to the Golgi
RT complex.";
RL EMBO J. 18:3934-3946(1999).
RN [11]
RP TISSUE SPECIFICITY.
RX PubMed=12414650;
RA Bourdon V., Naef F., Rao P.H., Reuter V., Mok S.C., Bosl G.J., Koul S.,
RA Murty V.V., Kucherlapati R.S., Chaganti R.S.;
RT "Genomic and expression analysis of the 12p11-p12 amplicon using EST arrays
RT identifies two novel amplified and overexpressed genes.";
RL Cancer Res. 62:6218-6223(2002).
RN [12]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22223895; DOI=10.1074/mcp.m111.015131;
RA Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T.,
RA Giglione C.;
RT "Comparative large-scale characterisation of plant vs. mammal proteins
RT reveals similar and idiosyncratic N-alpha acetylation features.";
RL Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012).
RN [13]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=25944712; DOI=10.1002/pmic.201400617;
RA Vaca Jacome A.S., Rabilloud T., Schaeffer-Reiss C., Rompais M., Ayoub D.,
RA Lane L., Bairoch A., Van Dorsselaer A., Carapito C.;
RT "N-terminome analysis of the human mitochondrial proteome.";
RL Proteomics 15:2519-2524(2015).
CC -!- FUNCTION: May be involved in fusion of ER-derived transport vesicles
CC with the Golgi complex.
CC -!- INTERACTION:
CC Q9Y3E0; Q96BA8: CREB3L1; NbExp=3; IntAct=EBI-4402607, EBI-6942903;
CC Q9Y3E0; Q8TDT2: GPR152; NbExp=3; IntAct=EBI-4402607, EBI-13345167;
CC Q9Y3E0; Q9BT40: INPP5K; NbExp=3; IntAct=EBI-4402607, EBI-749162;
CC Q9Y3E0; P15941-11: MUC1; NbExp=3; IntAct=EBI-4402607, EBI-17263240;
CC Q9Y3E0; Q9NR31: SAR1A; NbExp=3; IntAct=EBI-4402607, EBI-3920694;
CC Q9Y3E0; O00560: SDCBP; NbExp=3; IntAct=EBI-4402607, EBI-727004;
CC Q9Y3E0; Q8IVP1: SH3GL3; NbExp=3; IntAct=EBI-4402607, EBI-6503765;
CC Q9Y3E0; Q6UWV7: SHISAL2A; NbExp=3; IntAct=EBI-4402607, EBI-18396772;
CC Q9Y3E0; Q3KNW5: SLC10A6; NbExp=3; IntAct=EBI-4402607, EBI-18159983;
CC Q9Y3E0; Q9NZD8: SPG21; NbExp=3; IntAct=EBI-4402607, EBI-742688;
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000269|PubMed:10406798}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:10406798}.
CC -!- TISSUE SPECIFICITY: Widely expressed. Tends to be up-regulated in
CC seminomas compared to normal testis. {ECO:0000269|PubMed:12414650}.
CC -!- SIMILARITY: Belongs to the GOT1 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF65181.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AF151899; AAD34136.1; -; mRNA.
DR EMBL; AL136571; CAB66506.1; -; mRNA.
DR EMBL; AB097020; BAC77373.1; -; mRNA.
DR EMBL; AY358975; AAQ89334.1; -; mRNA.
DR EMBL; AF068292; AAF65181.1; ALT_INIT; mRNA.
DR EMBL; CR457389; CAG33670.1; -; mRNA.
DR EMBL; AK311920; BAG34861.1; -; mRNA.
DR EMBL; CH471094; EAW96443.1; -; Genomic_DNA.
DR EMBL; BC012455; AAH12455.1; -; mRNA.
DR CCDS; CCDS8689.1; -.
DR PIR; T46908; T46908.
DR RefSeq; NP_057156.1; NM_016072.4.
DR AlphaFoldDB; Q9Y3E0; -.
DR BioGRID; 119232; 223.
DR IntAct; Q9Y3E0; 192.
DR MINT; Q9Y3E0; -.
DR STRING; 9606.ENSP00000229314; -.
DR iPTMnet; Q9Y3E0; -.
DR MetOSite; Q9Y3E0; -.
DR PhosphoSitePlus; Q9Y3E0; -.
DR BioMuta; GOLT1B; -.
DR DMDM; 20534680; -.
DR EPD; Q9Y3E0; -.
DR jPOST; Q9Y3E0; -.
DR MassIVE; Q9Y3E0; -.
DR MaxQB; Q9Y3E0; -.
DR PaxDb; Q9Y3E0; -.
DR PeptideAtlas; Q9Y3E0; -.
DR PRIDE; Q9Y3E0; -.
DR ProteomicsDB; 86027; -.
DR TopDownProteomics; Q9Y3E0; -.
DR Antibodypedia; 67178; 60 antibodies from 17 providers.
DR DNASU; 51026; -.
DR Ensembl; ENST00000229314.10; ENSP00000229314.4; ENSG00000111711.10.
DR GeneID; 51026; -.
DR KEGG; hsa:51026; -.
DR MANE-Select; ENST00000229314.10; ENSP00000229314.4; NM_016072.5; NP_057156.1.
DR UCSC; uc001rez.3; human.
DR CTD; 51026; -.
DR DisGeNET; 51026; -.
DR GeneCards; GOLT1B; -.
DR HGNC; HGNC:20175; GOLT1B.
DR HPA; ENSG00000111711; Low tissue specificity.
DR MIM; 615078; gene.
DR neXtProt; NX_Q9Y3E0; -.
DR OpenTargets; ENSG00000111711; -.
DR PharmGKB; PA134898810; -.
DR VEuPathDB; HostDB:ENSG00000111711; -.
DR eggNOG; KOG1743; Eukaryota.
DR GeneTree; ENSGT00390000014507; -.
DR InParanoid; Q9Y3E0; -.
DR OMA; IFVRWPV; -.
DR OrthoDB; 1534843at2759; -.
DR PhylomeDB; Q9Y3E0; -.
DR TreeFam; TF300267; -.
DR PathwayCommons; Q9Y3E0; -.
DR SignaLink; Q9Y3E0; -.
DR BioGRID-ORCS; 51026; 145 hits in 1052 CRISPR screens.
DR ChiTaRS; GOLT1B; human.
DR GeneWiki; GOLT1B; -.
DR GenomeRNAi; 51026; -.
DR Pharos; Q9Y3E0; Tbio.
DR PRO; PR:Q9Y3E0; -.
DR Proteomes; UP000005640; Chromosome 12.
DR RNAct; Q9Y3E0; protein.
DR Bgee; ENSG00000111711; Expressed in secondary oocyte and 200 other tissues.
DR ExpressionAtlas; Q9Y3E0; baseline and differential.
DR Genevisible; Q9Y3E0; HS.
DR GO; GO:0005829; C:cytosol; IEA:GOC.
DR GO; GO:0005783; C:endoplasmic reticulum; IDA:LIFEdb.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; HDA:UniProtKB.
DR GO; GO:0032991; C:protein-containing complex; IDA:MGI.
DR GO; GO:0006888; P:endoplasmic reticulum to Golgi vesicle-mediated transport; IEA:InterPro.
DR GO; GO:0043123; P:positive regulation of I-kappaB kinase/NF-kappaB signaling; HMP:UniProtKB.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0042147; P:retrograde transport, endosome to Golgi; IEA:InterPro.
DR InterPro; IPR045176; Got1.
DR InterPro; IPR007305; Vesicle_transpt_Got1/SFT2.
DR PANTHER; PTHR21493; PTHR21493; 1.
DR Pfam; PF04178; Got1; 1.
PE 1: Evidence at protein level;
KW Acetylation; Golgi apparatus; Membrane; Protein transport;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..138
FT /note="Vesicle transport protein GOT1B"
FT /id="PRO_0000218582"
FT TOPO_DOM 1..9
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 10..30
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 31..32
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 33..53
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 54..68
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 69..89
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 90
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 91..109
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 110..138
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0007744|PubMed:22223895"
FT CONFLICT 37
FT /note="I -> T (in Ref. 5; AAF65181)"
FT /evidence="ECO:0000305"
FT CONFLICT 54
FT /note="R -> K (in Ref. 6; CAG33670)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 138 AA; 15426 MW; 2EB85823C34EFAFA CRC64;
MISLTDTQKI GMGLTGFGVF FLFFGMILFF DKALLAIGNV LFVAGLAFVI GLERTFRFFF
QKHKMKATGF FLGGVFVVLI GWPLIGMIFE IYGFFLLFRG FFPVVVGFIR RVPVLGSLLN
LPGIRSFVDK VGESNNMV
