GO_HCMVA
ID GO_HCMVA Reviewed; 466 AA.
AC P16750; Q7M6L8;
DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1990, sequence version 1.
DT 29-SEP-2021, entry version 60.
DE RecName: Full=Glycoprotein O;
DE Short=gO;
DE Flags: Precursor;
GN Name=GO; Synonyms=UL74;
OS Human cytomegalovirus (strain AD169) (HHV-5) (Human herpesvirus 5).
OC Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC Herpesvirales; Herpesviridae; Betaherpesvirinae; Cytomegalovirus.
OX NCBI_TaxID=10360;
OH NCBI_TaxID=9606; Homo sapiens (Human).
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=2161319; DOI=10.1007/978-3-642-74980-3_6;
RA Chee M.S., Bankier A.T., Beck S., Bohni R., Brown C.M., Cerny R.,
RA Horsnell T., Hutchison C.A. III, Kouzarides T., Martignetti J.A.,
RA Preddie E., Satchwell S.C., Tomlinson P., Weston K.M., Barrell B.G.;
RT "Analysis of the protein-coding content of the sequence of human
RT cytomegalovirus strain AD169.";
RL Curr. Top. Microbiol. Immunol. 154:125-169(1990).
RN [2]
RP PROTEIN SEQUENCE OF 189-195, SUBCELLULAR LOCATION, CHARACTERIZATION,
RP GLYCOSYLATION, AND IDENTIFICATION IN A COMPLEX WITH GL AND GH.
RX PubMed=9733861; DOI=10.1128/jvi.72.10.8191-8197.1998;
RA Huber M.T., Compton T.;
RT "The human cytomegalovirus UL74 gene encodes the third component of the
RT glycoprotein H-glycoprotein L-containing envelope complex.";
RL J. Virol. 72:8191-8197(1998).
RN [3]
RP GENOME REANNOTATION.
RX PubMed=12533697; DOI=10.1099/vir.0.18606-0;
RA Davison A.J., Dolan A., Akter P., Addison C., Dargan D.J., Alcendor D.J.,
RA McGeoch D.J., Hayward G.S.;
RT "The human cytomegalovirus genome revisited: comparison with the chimpanzee
RT cytomegalovirus genome.";
RL J. Gen. Virol. 84:17-28(2003).
RN [4]
RP ERRATUM OF PUBMED:12533697.
RA Davison A.J., Dolan A., Akter P., Addison C., Dargan D.J., Alcendor D.J.,
RA McGeoch D.J., Hayward G.S.;
RL J. Gen. Virol. 84:1053-1053(2003).
RN [5]
RP IDENTIFICATION.
RX PubMed=15452216; DOI=10.1128/jvi.78.20.10960-10966.2004;
RA Varnum S.M., Streblow D.N., Monroe M.E., Smith P., Auberry K.J.,
RA Pasa-Tolic L., Wang D., Camp D.G. II, Rodland K., Wiley S., Britt W.,
RA Shenk T., Smith R.D., Nelson J.A.;
RT "Identification of proteins in human cytomegalovirus (HCMV) particles: the
RT HCMV proteome.";
RL J. Virol. 78:10960-10966(2004).
RN [6]
RP ERRATUM OF PUBMED:15452216.
RA Varnum S.M., Streblow D.N., Monroe M.E., Smith P., Auberry K.J.,
RA Pasa-Tolic L., Wang D., Camp D.G. II, Rodland K., Wiley S., Britt W.,
RA Shenk T., Smith R.D., Nelson J.A.;
RL J. Virol. 78:13395-13395(2004).
CC -!- FUNCTION: Plays a role in viral entry into host cells. Forms a trimeric
CC complex at the surface of the viral envelope together with gH and gL.
CC This complex is required for entry in host fibroblasts.
CC Mechanistically, engages host receptor(s) including PDGFRA to mediate
CC infection. {ECO:0000250|UniProtKB:F5HGP1}.
CC -!- SUBUNIT: Forms the envelope trimer complex composed of gH, gL, and gO.
CC The trimer interacts with host PDGFRA. {ECO:0000250|UniProtKB:F5HGP1}.
CC -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000305|PubMed:9733861}.
CC Note=Host membrane associated, either via its interaction with gH, or
CC as a type II transmembrane protein. {ECO:0000305}.
CC -!- PTM: N-glycosylated. {ECO:0000269|PubMed:9733861}.
CC -!- PTM: The N-terminus is blocked.
CC -!- SIMILARITY: Belongs to the herpesviridae U47 family. {ECO:0000305}.
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DR EMBL; X17403; CAA35389.1; -; Genomic_DNA.
DR EMBL; BK000394; DAA00170.1; -; Genomic_DNA.
DR PIR; S09837; S09837.
DR SMR; P16750; -.
DR Proteomes; UP000008991; Genome.
DR Proteomes; UP000008992; Genome.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR InterPro; IPR012564; Herpes_UL74.
DR Pfam; PF07982; Herpes_UL74; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Glycoprotein; Membrane; Reference proteome;
KW Signal; Virion.
FT SIGNAL 1..30
FT /evidence="ECO:0000255"
FT CHAIN 31..466
FT /note="Glycoprotein O"
FT /id="PRO_0000038318"
FT CARBOHYD 75
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT CARBOHYD 83
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT CARBOHYD 87
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT CARBOHYD 103
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT CARBOHYD 130
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT CARBOHYD 157
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT CARBOHYD 162
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT CARBOHYD 171
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT CARBOHYD 219
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT CARBOHYD 242
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT CARBOHYD 288
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT CARBOHYD 292
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT CARBOHYD 350
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT CARBOHYD 385
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT CARBOHYD 392
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT CARBOHYD 399
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT CARBOHYD 433
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT CARBOHYD 454
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
SQ SEQUENCE 466 AA; 54235 MW; 20B931D97AB5D41D CRC64;
MGRKEMMVRD VPKMVFLISI SFLLVSFINC KVMSKALYNR PWRGLVLSKI GKYKLDQLKL
EILRQLETTI STKYNVSKQP VKNLTMNMTE FPQYYILAGP IQNYSITYLW FDFYSTQLRK
PAKYVYSQYN HTAKTITFRP PPCGTVPSMT CLSEMLNVSK RNDTGEQGCG NFTTFNPMFF
NVPRWNTKLY VGPTKVNVDS QTIYFLGLTA LLLRYAQRNC THSFYLVNAM SRNLFRVPKY
INGTKLKNTM RKLKRKQAPV KEQFEKKAKK TQSTTTPYFS YTTSAALNVT TNVTYSITTA
ARRVSTSTIA YRPDSSFMKS IMATQLRDLA TWVYTTLRYR QNPFCEPSRN RTAVSEFMKN
THVLIRNETP YTIYGTLDMS SLYYNETMFV ENKTASDSNK TTPTSPSMGF QRTFIDPLWD
YLDSLLFLDE IRNFSLRSPT YVNLTPPEHR RAVNLSTLNS LWWWLQ
