GP10_DICDI
ID GP10_DICDI Reviewed; 544 AA.
AC Q06885; Q55FB7;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1994, sequence version 1.
DT 25-MAY-2022, entry version 88.
DE RecName: Full=Glycoprotein gp100;
DE AltName: Full=P29F8;
DE Flags: Precursor;
GN Name=gppA; ORFNames=DDB_G0267398;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=8294503; DOI=10.1083/jcb.124.1.205;
RA Barth A., Mueller-Taubenberger A., Taranto P., Gerisch G.;
RT "Replacement of the phospholipid-anchor in the contact site A glycoprotein
RT of D. discoideum by a transmembrane region does not impede cell adhesion
RT but reduces residence time on the cell surface.";
RL J. Cell Biol. 124:205-215(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
CC -!- DEVELOPMENTAL STAGE: Restricted to the aggregation stage of development
CC in D.discoideum. No detectable activity in cell adhesion.
CC -!- PTM: N- and O-glycosylated.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; L04286; AAC37369.1; -; mRNA.
DR EMBL; AAFI02000003; EAL73151.1; -; Genomic_DNA.
DR RefSeq; XP_647616.1; XM_642524.1.
DR AlphaFoldDB; Q06885; -.
DR SMR; Q06885; -.
DR STRING; 44689.DDB0191207; -.
DR PaxDb; Q06885; -.
DR EnsemblProtists; EAL73151; EAL73151; DDB_G0267398.
DR GeneID; 8616428; -.
DR KEGG; ddi:DDB_G0267398; -.
DR dictyBase; DDB_G0267398; gppA.
DR eggNOG; ENOG502RHC1; Eukaryota.
DR HOGENOM; CLU_501026_0_0_1; -.
DR InParanoid; Q06885; -.
DR OMA; CENRINS; -.
DR PRO; PR:Q06885; -.
DR Proteomes; UP000002195; Chromosome 1.
DR GO; GO:0000331; C:contractile vacuole; IDA:dictyBase.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR InterPro; IPR014756; Ig_E-set.
DR SUPFAM; SSF81296; SSF81296; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Membrane; Reference proteome; Signal; Transmembrane;
KW Transmembrane helix.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT CHAIN 20..544
FT /note="Glycoprotein gp100"
FT /id="PRO_0000021342"
FT TOPO_DOM 20..489
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 490..510
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 511..544
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 84..215
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 444..480
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 84..101
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 162..208
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 450..465
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 80
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 224
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 308
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 332
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 366
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 380
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 410
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 422
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 478
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 544 AA; 59157 MW; 9B2C2CDE7C7D0AA0 CRC64;
MKNFILLVFL FLLVSNSLGK SNKKDDQSPE IVKINSNDHL PLYYEIIISG HFSIDKTGLT
VTYLGDKIHD FEFIKGQVLN VTGEPQNNPI PTVSINPDQL NDDRFEVSDS SDFSKQTPTP
TPTPTSKPTS TPTSTPSQTI PPTVSPTVPP QTTSPTSKPT STPTPTSTPT PTSTPTPTSQ
TIPPPTTTPK PTKSSKPTKT PVPTPTPTRP SSSVSKGYDI IKFNITDIED IEMGKITISY
KNGQSSSKHF QPNSIIKSIE RTNSVGGVVE FKGSFFYTDD YEPSIKIGNK ICETLTSSQT
SIRCYLTNGT GCGYTITIDN LLNPIDNNGN SNLTYCYANP IIDKVIGYKD KKLTLITIIG
KNFLNNATVI IEKPNGNKRN CSNVLLSTDT LFICELSKSY HTLSSPKTTN TTMLSFINNS
PNSTVSNEII EMISGYFQIK ISTKPSTTDD DNNKNNDDGD SEIDSVGKSA VDSSKSNNNS
GGGGNKKLYL LIILPTVLFI IVAALVAIFI KTRVSQNSGS KVNKNNNKKD SINVPFQMLD
EITT
