ID   GP12_BPLP2              Reviewed;          91 AA.
AC   D3WAD0;
DT   02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT   20-APR-2010, sequence version 1.
DT   02-JUN-2021, entry version 16.
DE   RecName: Full=Chaperone protein gp12 {ECO:0000305};
DE   AltName: Full=Gene product 12 {ECO:0000305};
DE            Short=gp12 {ECO:0000305};
OS   Lactococcus phage p2 (Lactococcus lactis bacteriophage p2).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Siphoviridae; Skunavirus.
OX   NCBI_TaxID=254252;
OH   NCBI_TaxID=1358; Lactococcus lactis.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Tremblay D.M., Deveau H., Moineau S.;
RT   "Complete genomic sequence of Lactococcus lactis phage p2.";
RL   Submitted (FEB-2010) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0007744|PDB:3D8L}
RP   X-RAY CRYSTALLOGRAPHY (2.90 ANGSTROMS), FUNCTION, SUBUNIT, AND INDUCTION.
RX   PubMed=19047351; DOI=10.1128/jb.01363-08;
RA   Siponen M., Sciara G., Villion M., Spinelli S., Lichiere J., Cambillau C.,
RA   Moineau S., Campanacci V.;
RT   "Crystal structure of ORF12 from Lactococcus lactis phage p2 identifies a
RT   tape measure protein chaperone.";
RL   J. Bacteriol. 191:728-734(2009).
CC   -!- FUNCTION: Probable chaperone for the tape measure protein. Might help
CC       to maintain the tape measure protein in solution during tail assembly.
CC       {ECO:0000305|PubMed:19047351}.
CC   -!- SUBUNIT: Homohexamer. Further self-assembles as a spiral.
CC       {ECO:0000269|PubMed:19047351}.
CC   -!- INDUCTION: Expressed in the late phase of the viral replicative cycle.
CC       {ECO:0000269|PubMed:19047351}.
CC   -!- SIMILARITY: Belongs to the skunalikevirus chaperone protein gp12
CC       family. {ECO:0000305}.
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DR   EMBL; GQ979703; ADC80087.1; -; Genomic_DNA.
DR   PDB; 3D8L; X-ray; 2.90 A; A/B/C=1-91.
DR   PDBsum; 3D8L; -.
DR   SMR; D3WAD0; -.
DR   Proteomes; UP000002348; Genome.
DR   GO; GO:0098003; P:viral tail assembly; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.8.940; -; 1.
DR   InterPro; IPR043077; Phage_p2_ORF12.
PE   1: Evidence at protein level;
KW   3D-structure; Late protein; Reference proteome;
KW   Viral release from host cell; Viral tail assembly.
FT   CHAIN           1..91
FT                   /note="Chaperone protein gp12"
FT                   /id="PRO_0000438232"
FT   HELIX           7..15
FT                   /evidence="ECO:0007829|PDB:3D8L"
FT   HELIX           19..31
FT                   /evidence="ECO:0007829|PDB:3D8L"
FT   HELIX           37..53
FT                   /evidence="ECO:0007829|PDB:3D8L"
FT   HELIX           57..70
FT                   /evidence="ECO:0007829|PDB:3D8L"
FT   HELIX           80..88
FT                   /evidence="ECO:0007829|PDB:3D8L"
SQ   SEQUENCE   91 AA;  10626 MW;  C1CA68AB9CBE313E CRC64;
     MAKQLSTARK FKMITGKDLF QQQKAMDTEL KKEDGEITDL MEFVQYGLYL ALFQDNIVKA
     KSDFSDFRSS FEFDTDGKGL KELVELWQKE I