3S1A_HYDTO
ID 3S1A_HYDTO Reviewed; 60 AA.
AC P0CG02;
DT 15-JUN-2010, integrated into UniProtKB/Swiss-Prot.
DT 15-JUN-2010, sequence version 1.
DT 25-MAY-2022, entry version 39.
DE RecName: Full=Toxin aagardi;
OS Hydrophis torquatus (West Coast black-headed sea snake) (Chitulia
OS torquata).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC Serpentes; Colubroidea; Hydrophiidae; Hydrophis.
OX NCBI_TaxID=747462;
RN [1]
RP PROTEIN SEQUENCE, SUBCELLULAR LOCATION, MASS SPECTROMETRY, AND TOXIC DOSE.
RC STRAIN=Subsp. aagaardi; TISSUE=Venom;
RX DOI=10.3390/toxins1020162;
RA Nagamizu M., Komori Y., Uchiya K.-I., Nikai T., Tu A.T.;
RT "Isolation and chemical characterization of a toxin isolated from the venom
RT of the sea snake, Hydrophis torquatus aagardi.";
RL Toxins 1:162-172(2009).
CC -!- FUNCTION: Binds to muscle nicotinic acetylcholine receptor (nAChR) and
CC inhibit acetylcholine from binding to the receptor, thereby impairing
CC neuromuscular transmission. {ECO:0000250|UniProtKB:P60775}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|Ref.1}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305}.
CC -!- MASS SPECTROMETRY: Mass=6591.1; Method=MALDI;
CC Evidence={ECO:0000269|Ref.1};
CC -!- TOXIC DOSE: LD(50) is 36 ng/kg by intravenous injection into mice.
CC {ECO:0000269|Ref.1}.
CC -!- SIMILARITY: Belongs to the snake three-finger toxin family. Short-chain
CC subfamily. Type I alpha-neurotoxin sub-subfamily. {ECO:0000305}.
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DR AlphaFoldDB; P0CG02; -.
DR SMR; P0CG02; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0030550; F:acetylcholine receptor inhibitor activity; IEA:UniProtKB-KW.
DR GO; GO:0099106; F:ion channel regulator activity; IEA:UniProtKB-KW.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR CDD; cd00206; snake_toxin; 1.
DR Gene3D; 2.10.60.10; -; 1.
DR InterPro; IPR003571; Snake_3FTx.
DR InterPro; IPR045860; Snake_toxin-like_sf.
DR InterPro; IPR018354; Snake_toxin_con_site.
DR SUPFAM; SSF57302; SSF57302; 1.
DR PROSITE; PS00272; SNAKE_TOXIN; 1.
PE 1: Evidence at protein level;
KW Acetylcholine receptor inhibiting toxin; Direct protein sequencing;
KW Disulfide bond; Ion channel impairing toxin; Neurotoxin;
KW Postsynaptic neurotoxin; Secreted; Toxin.
FT CHAIN 1..60
FT /note="Toxin aagardi"
FT /evidence="ECO:0000269|Ref.1"
FT /id="PRO_0000394533"
FT DISULFID 3..22
FT /evidence="ECO:0000250|UniProtKB:P0C1Z0"
FT DISULFID 17..39
FT /evidence="ECO:0000250|UniProtKB:P0C1Z0"
FT DISULFID 41..52
FT /evidence="ECO:0000250|UniProtKB:P0C1Z0"
FT DISULFID 53..58
FT /evidence="ECO:0000250|UniProtKB:P0C1Z0"
SQ SEQUENCE 60 AA; 6599 MW; DE41989F9056E59A CRC64;
MTCCNQQSSQ PKTTTNCAGN SCYKKTWSDH RGTIIERGCG CPQVKSGIKL ECCHTNECNN