ID   GP14_BPT7               Reviewed;         196 AA.
AC   P03724;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   03-AUG-2022, entry version 67.
DE   RecName: Full=Internal virion protein gp14 {ECO:0000255|HAMAP-Rule:MF_04118};
DE   AltName: Full=Gene product 14;
DE            Short=Gp14;
GN   OrderedLocusNames=14;
OS   Escherichia phage T7 (Bacteriophage T7).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Autographiviridae; Studiervirinae; Teseptimavirus.
OX   NCBI_TaxID=10760;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=6864790; DOI=10.1016/s0022-2836(83)80282-4;
RA   Dunn J.J., Studier F.W.;
RT   "Complete nucleotide sequence of bacteriophage T7 DNA and the locations of
RT   T7 genetic elements.";
RL   J. Mol. Biol. 166:477-535(1983).
RN   [2]
RP   SUBCELLULAR LOCATION.
RX   PubMed=20036409; DOI=10.1016/j.virol.2009.12.002;
RA   Chang C.Y., Kemp P., Molineux I.J.;
RT   "Gp15 and gp16 cooperate in translocating bacteriophage T7 DNA into the
RT   infected cell.";
RL   Virology 398:176-186(2010).
RN   [3]
RP   INTERACTION WITH THE PORTAL PROTEIN AND GP15.
RX   PubMed=23580619; DOI=10.1073/pnas.1215563110;
RA   Guo F., Liu Z., Vago F., Ren Y., Wu W., Wright E.T., Serwer P., Jiang W.;
RT   "Visualization of uncorrelated, tandem symmetry mismatches in the internal
RT   genome packaging apparatus of bacteriophage T7.";
RL   Proc. Natl. Acad. Sci. U.S.A. 110:6811-6816(2013).
RN   [4]
RP   SUBCELLULAR LOCATION.
RX   PubMed=23884409; DOI=10.1074/jbc.m113.491209;
RA   Cuervo A., Pulido-Cid M., Chagoyen M., Arranz R., Gonzalez-Garcia V.A.,
RA   Garcia-Doval C., Caston J.R., Valpuesta J.M., van Raaij M.J.,
RA   Martin-Benito J., Carrascosa J.L.;
RT   "Structural characterization of the bacteriophage T7 tail machinery.";
RL   J. Biol. Chem. 288:26290-26299(2013).
RN   [5]
RP   SUBCELLULAR LOCATION, AND FUNCTION.
RX   PubMed=23306440; DOI=10.1126/science.1231887;
RA   Hu B., Margolin W., Molineux I.J., Liu J.;
RT   "The bacteriophage t7 virion undergoes extensive structural remodeling
RT   during infection.";
RL   Science 339:576-579(2013).
CC   -!- FUNCTION: Component of the cylindrical core that assembles on the inner
CC       surface of the capsid during capsid formation and plays a role in viral
CC       DNA ejection into the host cell. The inner core is composed of stacked
CC       rings of gp14, gp15 and gp16 proteins. Following binding to the host
CC       cell surface, the internal core is disassembled and gp14 is ejected
CC       along with gp15 and gp16 into the infected cell. May form a simple
CC       channel spanning the outer membrane. {ECO:0000255|HAMAP-Rule:MF_04118,
CC       ECO:0000305|PubMed:23306440}.
CC   -!- SUBUNIT: Interacts with the portal protein. Interacts with gp15.
CC       {ECO:0000255|HAMAP-Rule:MF_04118, ECO:0000269|PubMed:23580619}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000255|HAMAP-Rule:MF_04118,
CC       ECO:0000269|PubMed:23884409}. Host cell outer membrane
CC       {ECO:0000255|HAMAP-Rule:MF_04118, ECO:0000269|PubMed:20036409,
CC       ECO:0000269|PubMed:23306440}.
CC   -!- SIMILARITY: Belongs to the T7virus internal virion protein gp14 family.
CC       {ECO:0000255|HAMAP-Rule:MF_04118}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; V01146; CAA24432.1; -; Genomic_DNA.
DR   PIR; A04350; NIBPB7.
DR   RefSeq; NP_042002.1; NC_001604.1.
DR   PDB; 7EY7; EM; 4.30 A; A/B/C/D/E/F=1-196.
DR   PDB; 7EYB; EM; 3.70 A; a/b/c/d/e/f/g/h=1-196.
DR   PDBsum; 7EY7; -.
DR   PDBsum; 7EYB; -.
DR   SMR; P03724; -.
DR   TCDB; 3.A.17.1.1; the phage t7 injectisome (t7 injectisome) family.
DR   GeneID; 1261032; -.
DR   KEGG; vg:1261032; -.
DR   Proteomes; UP000000840; Genome.
DR   GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0044423; C:virion component; IEA:UniProtKB-UniRule.
DR   GO; GO:0099002; P:viral genome ejection through host cell envelope, short tail mechanism; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_04118; GP14_T7; 1.
DR   InterPro; IPR038996; Gp14.
PE   1: Evidence at protein level;
KW   3D-structure; Host cell outer membrane; Host membrane; Membrane;
KW   Reference proteome; Viral genome ejection through host cell envelope;
KW   Viral penetration into host cytoplasm; Viral short tail ejection system;
KW   Virion; Virus entry into host cell.
FT   CHAIN           1..196
FT                   /note="Internal virion protein gp14"
FT                   /id="PRO_0000106528"
SQ   SEQUENCE   196 AA;  20968 MW;  FF7BC1AE3F18C868 CRC64;
     MCWAAAIPIA ISGAQAISGQ NAQAKMIAAQ TAAGRRQAME IMRQTNIQNA DLSLQARSKL
     EEASAELTSQ NMQKVQAIGS IRAAIGESML EGSSMDRIKR VTEGQFIREA NMVTENYRRD
     YQAIFAQQLG GTQSAASQID EIYKSEQKQK SKLQMVLDPL AIMGSSAASA YASGAFDSKS
     TTKAPIVAAK GTKTGR