GP16_BPLP2
ID GP16_BPLP2 Reviewed; 375 AA.
AC D3KFX4; D3WAD4;
DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 23-MAR-2010, sequence version 1.
DT 29-SEP-2021, entry version 26.
DE RecName: Full=Baseplate protein gp16 {ECO:0000303|PubMed:20351260};
DE AltName: Full=Gene product 16 {ECO:0000305};
DE Short=Gp16 {ECO:0000305};
DE AltName: Full=Tail-associated lysine-like protein {ECO:0000303|PubMed:24027307};
OS Lactococcus phage p2 (Lactococcus lactis bacteriophage p2).
OC Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC Caudovirales; Siphoviridae; Skunavirus.
OX NCBI_TaxID=254252;
OH NCBI_TaxID=1358; Lactococcus lactis.
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Tremblay D.M., Deveau H., Moineau S.;
RT "Complete genomic sequence of Lactococcus lactis phage p2.";
RL Submitted (FEB-2010) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0007744|PDB:2WZP}
RP X-RAY CRYSTALLOGRAPHY (2.60 ANGSTROMS), SUBCELLULAR LOCATION, SUBUNIT, AND
RP FUNCTION.
RX PubMed=20351260; DOI=10.1073/pnas.1000232107;
RA Sciara G., Bebeacua C., Bron P., Tremblay D., Ortiz-Lombardia M.,
RA Lichiere J., van Heel M., Campanacci V., Moineau S., Cambillau C.;
RT "Structure of lactococcal phage p2 baseplate and its mechanism of
RT activation.";
RL Proc. Natl. Acad. Sci. U.S.A. 107:6852-6857(2010).
RN [3]
RP STRUCTURE BY ELECTRON MICROSCOPY (22 ANGSTROMS) OF THE TAIL, AND
RP SUBCELLULAR LOCATION.
RX PubMed=24027307; DOI=10.1128/jvi.02033-13;
RA Bebeacua C., Tremblay D., Farenc C., Chapot-Chartier M.P., Sadovskaya I.,
RA van Heel M., Veesler D., Moineau S., Cambillau C.;
RT "Structure, adsorption to host, and infection mechanism of virulent
RT lactococcal phage p2.";
RL J. Virol. 87:12302-12312(2013).
CC -!- FUNCTION: Forms a dome thereby closing the central channel at the end
CC of the baseplate. Changes its conformation upon activation by calcium
CC allowing the channel to open at the bottom of the baseplate for DNA
CC ejection. {ECO:0000269|PubMed:20351260}.
CC -!- SUBUNIT: Homotrimer. {ECO:0000269|PubMed:20351260}.
CC -!- INTERACTION:
CC D3KFX4; D3WAD3; NbExp=8; IntAct=EBI-15845184, EBI-15845172;
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000269|PubMed:20351260,
CC ECO:0000269|PubMed:24027307}. Note=Part of the baseplate.
CC {ECO:0000269|PubMed:20351260}.
CC -!- SIMILARITY: Belongs to the skunalikevirus baseplate protein gp16
CC family. {ECO:0000305}.
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DR EMBL; GQ979703; ADC80091.1; -; Genomic_DNA.
DR PDB; 2WZP; X-ray; 2.60 A; R=1-375.
DR PDB; 6ZIG; EM; 42.20 A; 1/Y/Z=1-375.
DR PDB; 6ZIH; EM; 28.70 A; 1/Y/Z=1-375.
DR PDB; 6ZJJ; EM; 22.00 A; 1/Y/Z=1-375.
DR PDBsum; 2WZP; -.
DR PDBsum; 6ZIG; -.
DR PDBsum; 6ZIH; -.
DR PDBsum; 6ZJJ; -.
DR SMR; D3KFX4; -.
DR DIP; DIP-59524N; -.
DR IntAct; D3KFX4; 1.
DR EvolutionaryTrace; D3KFX4; -.
DR Proteomes; UP000002348; Genome.
DR GO; GO:0098025; C:virus tail, baseplate; IEA:UniProtKB-KW.
DR GO; GO:0099001; P:viral genome ejection through host cell envelope, long flexible tail mechanism; IEA:UniProtKB-KW.
DR Gene3D; 2.30.300.20; -; 1.
DR Gene3D; 3.55.50.50; -; 1.
DR InterPro; IPR031861; Caudo_ORF16.
DR InterPro; IPR043075; Gp16_dom1_2.
DR InterPro; IPR043073; Gp16_domD4.
DR Pfam; PF16792; Caudo_bapla16; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Reference proteome; Viral baseplate protein;
KW Viral genome ejection through host cell envelope;
KW Viral long flexible tail ejection system;
KW Viral penetration into host cytoplasm; Viral tail protein; Virion;
KW Virus entry into host cell.
FT CHAIN 1..375
FT /note="Baseplate protein gp16"
FT /id="PRO_0000438235"
FT STRAND 2..8
FT /evidence="ECO:0007829|PDB:2WZP"
FT HELIX 12..15
FT /evidence="ECO:0007829|PDB:2WZP"
FT STRAND 36..49
FT /evidence="ECO:0007829|PDB:2WZP"
FT STRAND 55..61
FT /evidence="ECO:0007829|PDB:2WZP"
FT STRAND 68..80
FT /evidence="ECO:0007829|PDB:2WZP"
FT STRAND 82..84
FT /evidence="ECO:0007829|PDB:2WZP"
FT STRAND 86..100
FT /evidence="ECO:0007829|PDB:2WZP"
FT STRAND 104..110
FT /evidence="ECO:0007829|PDB:2WZP"
FT HELIX 111..115
FT /evidence="ECO:0007829|PDB:2WZP"
FT TURN 116..118
FT /evidence="ECO:0007829|PDB:2WZP"
FT HELIX 123..126
FT /evidence="ECO:0007829|PDB:2WZP"
FT STRAND 127..129
FT /evidence="ECO:0007829|PDB:2WZP"
FT HELIX 130..137
FT /evidence="ECO:0007829|PDB:2WZP"
FT TURN 140..142
FT /evidence="ECO:0007829|PDB:2WZP"
FT STRAND 150..152
FT /evidence="ECO:0007829|PDB:2WZP"
FT HELIX 154..157
FT /evidence="ECO:0007829|PDB:2WZP"
FT HELIX 165..167
FT /evidence="ECO:0007829|PDB:2WZP"
FT HELIX 172..183
FT /evidence="ECO:0007829|PDB:2WZP"
FT STRAND 188..193
FT /evidence="ECO:0007829|PDB:2WZP"
FT STRAND 202..208
FT /evidence="ECO:0007829|PDB:2WZP"
FT STRAND 211..214
FT /evidence="ECO:0007829|PDB:2WZP"
FT STRAND 218..222
FT /evidence="ECO:0007829|PDB:2WZP"
FT HELIX 224..226
FT /evidence="ECO:0007829|PDB:2WZP"
FT STRAND 229..232
FT /evidence="ECO:0007829|PDB:2WZP"
FT TURN 235..237
FT /evidence="ECO:0007829|PDB:2WZP"
FT STRAND 242..248
FT /evidence="ECO:0007829|PDB:2WZP"
FT TURN 251..254
FT /evidence="ECO:0007829|PDB:2WZP"
FT STRAND 261..265
FT /evidence="ECO:0007829|PDB:2WZP"
FT STRAND 271..273
FT /evidence="ECO:0007829|PDB:2WZP"
FT HELIX 274..276
FT /evidence="ECO:0007829|PDB:2WZP"
FT STRAND 290..295
FT /evidence="ECO:0007829|PDB:2WZP"
FT HELIX 306..310
FT /evidence="ECO:0007829|PDB:2WZP"
FT STRAND 320..325
FT /evidence="ECO:0007829|PDB:2WZP"
FT STRAND 337..342
FT /evidence="ECO:0007829|PDB:2WZP"
FT STRAND 345..356
FT /evidence="ECO:0007829|PDB:2WZP"
FT STRAND 361..366
FT /evidence="ECO:0007829|PDB:2WZP"
FT TURN 371..373
FT /evidence="ECO:0007829|PDB:2WZP"
SQ SEQUENCE 375 AA; 42837 MW; D07CB9AFE7A4F20F CRC64;
MLEANVYDNF NPNYYNISDF SMPNGKKEKR GLPIPKARCQ VINYELWETG YLYTSSATLT
VSVEVGDIVQ ILFPEVVPIE EALGKKKKLN LDMVYLVTDV DESNKATLKN YFWAMIESLD
VPNAITKTTN FAIIDYLIDP NKNNLMSYGY FFNSSIFAGK ATINRKAETS SAHDVAKRIF
SKVQFQPTTT IQHAPSETDP RNLLFINFAS RNWNRKRITT RVDIKQSVTM DTETIVERSA
YNFAVVFVKN KATDDYTDPP KMYIAKNNGD VIDYSTYHGD GTDLPDVRTA KTLFYDRDDH
GNPPELSTIK VEISPSTIVT RLIFNQNELL PLYVNDLVDI WYEGKLYSGY IADRVKTEFN
DRLIFVESGD KPNVI
