ID   GP16_BPP22              Reviewed;         609 AA.
AC   Q01146; A0A2H4A344; A8CGD6; Q8LTG2;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   20-JUN-2018, sequence version 2.
DT   23-FEB-2022, entry version 69.
DE   RecName: Full=Internal virion protein gp16 {ECO:0000305};
DE   AltName: Full=Ejection protein gp16;
DE            Short=E protein gp16;
GN   Name=16;
OS   Salmonella phage P22 (Bacteriophage P22).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Podoviridae; Lederbergvirus.
OX   NCBI_TaxID=10754;
OH   NCBI_TaxID=90371; Salmonella typhimurium.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1585633; DOI=10.1016/0042-6822(92)90503-h;
RA   Umlauf B., Dreiseikelmann B.;
RT   "Cloning, sequencing, and overexpression of gene 16 of Salmonella
RT   bacteriophage P22.";
RL   Virology 188:495-501(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=11053393; DOI=10.1128/jb.182.22.6472-6481.2000;
RA   Vander Byl C.S., Kropinski A.M.B.;
RT   "Sequence of the genome of Salmonella bacteriophage P22.";
RL   J. Bacteriol. 182:6472-6481(2000).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=12562822; DOI=10.1128/jb.185.4.1475-1477.2003;
RA   Pedulla M.L., Ford M.E., Karthikeyan T., Houtz J.M., Hendrix R.W.,
RA   Hatfull G.F., Poteete A.R., Gilcrease E.B., Winn-Stapley D.A.,
RA   Casjens S.R.;
RT   "Corrected sequence of the bacteriophage P22 genome.";
RL   J. Bacteriol. 185:1475-1477(2003).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MSU;
RX   PubMed=18621868; DOI=10.1128/aem.00352-08;
RA   Masago Y., Shibata T., Rose J.B.;
RT   "Bacteriophage P22 and Staphylococcus aureus attenuation on nonporous
RT   fomites as determined by plate assay and quantitative PCR.";
RL   Appl. Environ. Microbiol. 74:5838-5840(2008).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 19585-B1;
RA   Masago Y., Fong T.T., Rose J.B.;
RT   "The Molecular Identity of Contaminant Hydrology (20 years later).";
RL   Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases.
RN   [6]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-3.
RX   PubMed=8464750; DOI=10.1093/nar/21.6.1499;
RA   Adhikari P., Berget P.B.;
RT   "Sequence of a DNA injection gene from Salmonella typhimurium phage P22.";
RL   Nucleic Acids Res. 21:1499-1499(1993).
RN   [7]
RP   PROTEIN SEQUENCE OF 1-8.
RX   PubMed=1853558; DOI=10.1016/0042-6822(91)90981-g;
RA   Eppler K., Wyckoff E., Goates J., Parr R., Casjens S.;
RT   "Nucleotide sequence of the bacteriophage P22 genes required for DNA
RT   packaging.";
RL   Virology 183:519-538(1991).
RN   [8]
RP   SUBCELLULAR LOCATION.
RX   PubMed=328927; DOI=10.1128/jvi.23.1.91-97.1977;
RA   Israel V.;
RT   "E proteins of bacteriophage P22. I. Identification and ejection from wild-
RT   type and defective particles.";
RL   J. Virol. 23:91-97(1977).
RN   [9]
RP   FUNCTION.
RX   PubMed=26245366; DOI=10.1016/j.virol.2015.07.006;
RA   Jin Y., Sdao S.M., Dover J.A., Porcek N.B., Knobler C.M., Gelbart W.M.,
RA   Parent K.N.;
RT   "Bacteriophage P22 ejects all of its internal proteins before its genome.";
RL   Virology 485:128-134(2015).
RN   [10]
RP   STRUCTURE BY ELECTRON MICROSCOPY (12.5 ANGSTROMS) OF THE VIRION, AND
RP   FUNCTION.
RX   PubMed=29590587; DOI=10.1016/j.bpj.2018.01.026;
RA   McNulty R., Cardone G., Gilcrease E.B., Baker T.S., Casjens S.R.,
RA   Johnson J.E.;
RT   "Cryo-EM Elucidation of the Structure of Bacteriophage P22 Virions after
RT   Genome Release.";
RL   Biophys. J. 114:1295-1301(2018).
CC   -!- FUNCTION: Inner capsid protein that plays a role in viral DNA ejection
CC       into the host cell. {ECO:0000269|PubMed:26245366,
CC       ECO:0000269|PubMed:29590587}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000269|PubMed:328927}.
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DR   EMBL; M74136; AAA79250.1; -; Genomic_DNA.
DR   EMBL; AF217253; AAF75055.1; -; Genomic_DNA.
DR   EMBL; BK000583; DAA00995.1; -; Genomic_DNA.
DR   EMBL; AB362338; BAF80728.1; -; Genomic_DNA.
DR   EMBL; AB426868; BAG12611.1; -; Genomic_DNA.
DR   EMBL; AF527608; AAM81397.1; -; Genomic_DNA.
DR   EMBL; L07556; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   PIR; A42537; A42537.
DR   RefSeq; YP_063718.1; NC_002371.2.
DR   SMR; Q01146; -.
DR   TCDB; 1.K.4.1.1; the phage p22 injectisome (p22 injectisome) family.
DR   GeneID; 2944238; -.
DR   KEGG; vg:2944238; -.
DR   Proteomes; UP000001315; Genome.
DR   Proteomes; UP000001795; Genome.
DR   Proteomes; UP000001796; Genome.
DR   Proteomes; UP000002165; Genome.
DR   Proteomes; UP000007960; Genome.
DR   GO; GO:0099002; P:viral genome ejection through host cell envelope, short tail mechanism; IDA:UniProtKB.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Late protein; Reference proteome;
KW   Viral genome ejection through host cell envelope;
KW   Viral penetration into host cytoplasm; Viral short tail ejection system;
KW   Virion; Virus entry into host cell.
FT   CHAIN           1..609
FT                   /note="Internal virion protein gp16"
FT                   /id="PRO_0000077762"
FT   REGION          32..52
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        36..52
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        396
FT                   /note="D -> A (in Ref. 1; AAA79250 and 2; AAF75055)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        398
FT                   /note="A -> S (in Ref. 1; AAA79250 and 2; AAF75055)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   609 AA;  64386 MW;  019E28AB1D95F2ED CRC64;
     MKVTANGKTF NFPDGTSTED IGAAVDEYFA GQASAAETQP AEQQEEPQQP EQSLMQRAGD
     LLTGGQSAGQ IAEQAGRGLV NIPFDVLQGG ASLINAISQG LGGPKVLDDV YRPVDRPTDP
     YAQAGESIGG YLIPGAGVAG NMAIGSVAEA ANQQGDFAGN VAKNAAVNLG AQGLLSGAAK
     LVGRGITAAR GEIAPEARQL IDTAESMGVK PMTSDMIKPG NAFTRSLMQG GEGALLGTGG
     KRAEQYAIRS KLLGDYFDRV GGYNPDDIVK SMTSTVGGRK NAAGAVRDEI VNRMGSAPVG
     TTNSINAIDT NIARLEKLGT SADQRLLTAL KNLKGELNSG NVDFDLLQQH RTAFRTNVQG
     DAMVFPNQAK AATNMVENAM TRDLRNAVGK SLGPQDAAKY LKSNSDFANI YNKVLNKRIS
     NTLNKARSEY TPELINTVVF SRKPSDIKRI WSSLDNKGKD AMRAAYISKI AEKTGDSPAK
     FITEVNKLKA QSGGEIYNTI FSGRHMKELD ALHDVLRQTA RSDSANVVTQ TGQALANPVR
     LGAAIPTLGK SLAAEAGYGL AMRVYESKPI RNMLLRLANT KPGTPAYERA LNQAATAVRP
     LLANEATRQ