GP171_HUMAN
ID GP171_HUMAN Reviewed; 319 AA.
AC O14626; D3DNJ4; Q8IV06;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 161.
DE RecName: Full=G-protein coupled receptor 171;
DE AltName: Full=G-protein coupled receptor H963;
GN Name=GPR171 {ECO:0000312|HGNC:HGNC:30057}; Synonyms=H963;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Peripheral blood monocyte;
RX PubMed=9370294; DOI=10.1016/s0378-1119(97)00330-2;
RA Jacobs K.A., Collins-Racie L.A., Colbert M., Duckett M., Golden-Fleet M.,
RA Kelleher K., Kriz R., LaVallie E.R., Merberg D., Spaulding V., Stover J.,
RA Williamson M.J., McCoy J.M.;
RT "A genetic selection for isolating cDNAs encoding secreted proteins.";
RL Gene 198:289-296(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=11524702; DOI=10.1086/323610;
RA Joensuu T., Haemaelaeinen R., Yuan B., Johnson C., Tegelberg S.,
RA Gasparini P., Zelante L., Pirvola U., Pakarinen L., Lehesjoki A.-E.,
RA de la Chapelle A., Sankila E.-M.;
RT "Mutations in a novel gene with transmembrane domains underlie Usher
RT syndrome type 3.";
RL Am. J. Hum. Genet. 69:673-684(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT VAL-283.
RC TISSUE=Pancreas;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=34615877; DOI=10.1038/s41467-021-26135-9;
RA Fujiwara Y., Torphy R.J., Sun Y., Miller E.N., Ho F., Borcherding N.,
RA Wu T., Torres R.M., Zhang W., Schulick R.D., Zhu Y.;
RT "The GPR171 pathway suppresses T cell activation and limits antitumor
RT immunity.";
RL Nat. Commun. 12:5857-5857(2021).
CC -!- FUNCTION: G-protein coupled receptor for Big LEN, a 16-amino acid
CC neuropeptide produced from the precursor protein, proSAAS (encoded by
CC PCSK1N). Acts through a G(i)-alpha-mediated pathway in response to Big
CC LEN. Big LEN-GPR171 system plays an important role in regulating
CC feeding and metabolism. Also plays a role in modulating fear and
CC anxiety-like behaviors in the basolateral amygdala. Big LEN-GPR171
CC modulates the mu-type opioid receptor signaling and antinociception (By
CC similarity). Acts as a negative regulator T cell function
CC (PubMed:34615877). {ECO:0000250|UniProtKB:Q8BG55,
CC ECO:0000269|PubMed:34615877}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q8BG55};
CC Multi-pass membrane protein {ECO:0000255}. Note=Colocalized with GPR83
CC in the paraventricular nucleus. {ECO:0000250|UniProtKB:Q8BG55}.
CC -!- TISSUE SPECIFICITY: Expressed in both T-cell subsets and natural killer
CC cells, while it is undetectable in B cells or CD14(+) monocytes.
CC Expressed in peripheral blood mononuclear cells (PBMC) and Jurkat cells
CC (at protein level). {ECO:0000269|PubMed:34615877}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC {ECO:0000255|PROSITE-ProRule:PRU00521}.
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DR EMBL; AF002986; AAC51846.1; -; mRNA.
DR EMBL; AF411849; AAL47763.1; -; Genomic_DNA.
DR EMBL; CH471052; EAW78811.1; -; Genomic_DNA.
DR EMBL; CH471052; EAW78812.1; -; Genomic_DNA.
DR EMBL; BC036815; AAH36815.1; -; mRNA.
DR CCDS; CCDS3155.1; -.
DR RefSeq; NP_037440.3; NM_013308.3.
DR RefSeq; XP_005247459.1; XM_005247402.3.
DR RefSeq; XP_005247460.1; XM_005247403.3.
DR RefSeq; XP_016861763.1; XM_017006274.1.
DR AlphaFoldDB; O14626; -.
DR SMR; O14626; -.
DR BioGRID; 118956; 2.
DR IntAct; O14626; 1.
DR STRING; 9606.ENSP00000308479; -.
DR ChEMBL; CHEMBL4523860; -.
DR GlyGen; O14626; 1 site.
DR PhosphoSitePlus; O14626; -.
DR BioMuta; GPR171; -.
DR MassIVE; O14626; -.
DR PaxDb; O14626; -.
DR PeptideAtlas; O14626; -.
DR PRIDE; O14626; -.
DR ProteomicsDB; 48124; -.
DR Antibodypedia; 18294; 323 antibodies from 31 providers.
DR DNASU; 29909; -.
DR Ensembl; ENST00000309180.6; ENSP00000308479.5; ENSG00000174946.7.
DR Ensembl; ENST00000617554.1; ENSP00000482588.1; ENSG00000174946.7.
DR GeneID; 29909; -.
DR KEGG; hsa:29909; -.
DR MANE-Select; ENST00000309180.6; ENSP00000308479.5; NM_013308.4; NP_037440.3.
DR UCSC; uc003eyq.5; human.
DR CTD; 29909; -.
DR DisGeNET; 29909; -.
DR GeneCards; GPR171; -.
DR HGNC; HGNC:30057; GPR171.
DR HPA; ENSG00000174946; Tissue enhanced (lymphoid).
DR MIM; 618925; gene.
DR neXtProt; NX_O14626; -.
DR OpenTargets; ENSG00000174946; -.
DR PharmGKB; PA134948560; -.
DR VEuPathDB; HostDB:ENSG00000174946; -.
DR eggNOG; ENOG502QTCA; Eukaryota.
DR GeneTree; ENSGT01050000244845; -.
DR HOGENOM; CLU_009579_8_2_1; -.
DR InParanoid; O14626; -.
DR OMA; WHVFTNF; -.
DR OrthoDB; 1087125at2759; -.
DR PhylomeDB; O14626; -.
DR TreeFam; TF330969; -.
DR PathwayCommons; O14626; -.
DR SignaLink; O14626; -.
DR BioGRID-ORCS; 29909; 8 hits in 1059 CRISPR screens.
DR ChiTaRS; GPR171; human.
DR GeneWiki; GPR171; -.
DR GenomeRNAi; 29909; -.
DR Pharos; O14626; Tbio.
DR PRO; PR:O14626; -.
DR Proteomes; UP000005640; Chromosome 3.
DR RNAct; O14626; protein.
DR Bgee; ENSG00000174946; Expressed in buccal mucosa cell and 125 other tissues.
DR ExpressionAtlas; O14626; baseline and differential.
DR Genevisible; O14626; HS.
DR GO; GO:0016021; C:integral component of membrane; NAS:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008528; F:G protein-coupled peptide receptor activity; ISS:UniProtKB.
DR GO; GO:0045028; F:G protein-coupled purinergic nucleotide receptor activity; IBA:GO_Central.
DR GO; GO:0004930; F:G protein-coupled receptor activity; NAS:UniProtKB.
DR GO; GO:0042923; F:neuropeptide binding; ISS:UniProtKB.
DR GO; GO:0008188; F:neuropeptide receptor activity; ISS:UniProtKB.
DR GO; GO:0007188; P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway; ISS:UniProtKB.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; ISS:UniProtKB.
DR GO; GO:0045638; P:negative regulation of myeloid cell differentiation; IEA:Ensembl.
DR GO; GO:0060259; P:regulation of feeding behavior; ISS:UniProtKB.
DR GO; GO:0051930; P:regulation of sensory perception of pain; ISS:UniProtKB.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 1: Evidence at protein level;
KW Cell membrane; G-protein coupled receptor; Glycoprotein; Membrane;
KW Receptor; Reference proteome; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..319
FT /note="G-protein coupled receptor 171"
FT /id="PRO_0000069649"
FT TOPO_DOM 1..21
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 22..42
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 43..48
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 49..69
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 70..89
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 90..110
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 111..132
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 133..153
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 154..181
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 182..202
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 203..224
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 225..245
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 246..268
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 269..289
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 290..319
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 3
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VARIANT 283
FT /note="I -> V (in dbSNP:rs3732756)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT /id="VAR_049408"
SQ SEQUENCE 319 AA; 36754 MW; 79B9821C10841114 CRC64;
MTNSSFFCPV YKDLEPFTYF FYLVFLVGII GSCFATWAFI QKNTNHRCVS IYLINLLTAD
FLLTLALPVK IVVDLGVAPW KLKIFHCQVT ACLIYINMYL SIIFLAFVSI DRCLQLTHSC
KIYRIQEPGF AKMISTVVWL MVLLIMVPNM MIPIKDIKEK SNVGCMEFKK EFGRNWHLLT
NFICVAIFLN FSAIILISNC LVIRQLYRNK DNENYPNVKK ALINILLVTT GYIICFVPYH
IVRIPYTLSQ TEVITDCSTR ISLFKAKEAT LLLAVSNLCF DPILYYHLSK AFRSKVTETF
ASPKETKAQK EKLRCENNA
