GP171_RAT
ID GP171_RAT Reviewed; 326 AA.
AC D4A4Q2;
DT 25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT 20-APR-2010, sequence version 1.
DT 03-AUG-2022, entry version 83.
DE RecName: Full=G-protein coupled receptor 171;
GN Name=Gpr171 {ECO:0000312|RGD:1585271};
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Brown Norway;
RX PubMed=15057822; DOI=10.1038/nature02426;
RA Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J.,
RA Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G.,
RA Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G.,
RA Morgan M., Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G.,
RA Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S.,
RA Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T.,
RA Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D.,
RA Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L.,
RA Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D.,
RA Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M.,
RA Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C.,
RA Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J.,
RA Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H.,
RA Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X.,
RA Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q.,
RA Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P.,
RA Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A.,
RA Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C.,
RA Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J.,
RA Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J.,
RA Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F.,
RA Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A.,
RA Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A.,
RA Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J.,
RA Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E.,
RA Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M.,
RA Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C.,
RA Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L.,
RA Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W.,
RA Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y.,
RA Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V.,
RA Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M.,
RA Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S.,
RA Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B.,
RA Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R.,
RA Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J.,
RA Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D.,
RA Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S.,
RA Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S.,
RA Mockrin S., Collins F.S.;
RT "Genome sequence of the Brown Norway rat yields insights into mammalian
RT evolution.";
RL Nature 428:493-521(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=24043826; DOI=10.1073/pnas.1312938110;
RA Gomes I., Aryal D.K., Wardman J.H., Gupta A., Gagnidze K., Rodriguiz R.M.,
RA Kumar S., Wetsel W.C., Pintar J.E., Fricker L.D., Devi L.A.;
RT "GPR171 is a hypothalamic G protein-coupled receptor for BigLEN, a
RT neuropeptide involved in feeding.";
RL Proc. Natl. Acad. Sci. U.S.A. 110:16211-16216(2013).
CC -!- FUNCTION: G-protein coupled receptor for Big LEN, a 16-amino acid
CC neuropeptide produced from the precursor protein, proSAAS (encoded by
CC PCSK1N) (PubMed:24043826). Acts through a G(i)-alpha-mediated pathway
CC in response to Big LEN. Big LEN-GPR171 system plays an important role
CC in regulating feeding and metabolism. Also plays a role in modulating
CC fear and anxiety-like behaviors in the basolateral amygdala. Big LEN-
CC GPR171 modulates the mu-type opioid receptor signaling and
CC antinociception. Acts as a negative regulator T cell function (By
CC similarity). {ECO:0000250|UniProtKB:Q8BG55,
CC ECO:0000269|PubMed:24043826}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:24043826};
CC Multi-pass membrane protein {ECO:0000255}. Note=Colocalized with GPR83
CC in the paraventricular nucleus. {ECO:0000250|UniProtKB:Q8BG55}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC {ECO:0000255|PROSITE-ProRule:PRU00521}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AC128510; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH474003; EDM14855.1; -; Genomic_DNA.
DR RefSeq; NP_001102980.1; NM_001109510.1.
DR RefSeq; XP_017446606.1; XM_017591117.1.
DR AlphaFoldDB; D4A4Q2; -.
DR SMR; D4A4Q2; -.
DR STRING; 10116.ENSRNOP00000038911; -.
DR PaxDb; D4A4Q2; -.
DR Ensembl; ENSRNOT00000036690; ENSRNOP00000038911; ENSRNOG00000025297.
DR Ensembl; ENSRNOT00000102875; ENSRNOP00000080055; ENSRNOG00000025297.
DR GeneID; 688737; -.
DR KEGG; rno:688737; -.
DR UCSC; RGD:1585271; rat.
DR CTD; 29909; -.
DR RGD; 1585271; Gpr171.
DR eggNOG; ENOG502QTCA; Eukaryota.
DR GeneTree; ENSGT01050000244845; -.
DR HOGENOM; CLU_009579_8_2_1; -.
DR InParanoid; D4A4Q2; -.
DR OMA; WHVFTNF; -.
DR OrthoDB; 1087125at2759; -.
DR PhylomeDB; D4A4Q2; -.
DR TreeFam; TF330969; -.
DR Proteomes; UP000002494; Chromosome 2.
DR Proteomes; UP000234681; Chromosome 2.
DR Bgee; ENSRNOG00000025297; Expressed in spleen and 16 other tissues.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR GO; GO:0008528; F:G protein-coupled peptide receptor activity; ISS:UniProtKB.
DR GO; GO:0045028; F:G protein-coupled purinergic nucleotide receptor activity; IBA:GO_Central.
DR GO; GO:0042923; F:neuropeptide binding; ISS:UniProtKB.
DR GO; GO:0008188; F:neuropeptide receptor activity; ISS:UniProtKB.
DR GO; GO:0007188; P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway; ISS:UniProtKB.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IDA:UniProtKB.
DR GO; GO:0045638; P:negative regulation of myeloid cell differentiation; ISO:RGD.
DR GO; GO:0060259; P:regulation of feeding behavior; ISS:UniProtKB.
DR GO; GO:0051930; P:regulation of sensory perception of pain; ISS:UniProtKB.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 3: Inferred from homology;
KW Cell membrane; Disulfide bond; G-protein coupled receptor; Membrane;
KW Receptor; Reference proteome; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..326
FT /note="G-protein coupled receptor 171"
FT /id="PRO_0000455587"
FT TOPO_DOM 1..19
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 20..40
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 41..48
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 49..69
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 70..89
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 90..110
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 111..133
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 134..154
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 155..182
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 183..203
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 204..221
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 222..242
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 243..268
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 269..289
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 290..326
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT REGION 305..326
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT DISULFID 87..165
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
SQ SEQUENCE 326 AA; 37405 MW; 1250E198FD902E43 CRC64;
MTNSSMFCPI YRDLEPFTYF FYLVYLIGII GSCFATWAFI QKSTNHRCVS IYLINLLTAD
FLLTLALPVK IVVDLGVAPW KLRIFHCQVT ACLIYINMYL SIIFLAFVSI DRCLQLVHSC
KIYRIQEPGF AKMISAVVWL MVLLIMVPNM VIPIKNIKEK SNVGCMEFKR EFGKNWHLLT
NFICVAIFLN FSAIILISNF LVIRQLYRNR DNANYPSVKS ALLNILLVTA SYIICFVPYH
AVRIPYTLSQ TEVISDCSTR IALFKAKEAT LLLAVSNLCF DPILYYHLSK AFRLKVTETF
ASPQKMKARE EKPRRENDVQ STGSAC
