GP176_HUMAN
ID GP176_HUMAN Reviewed; 515 AA.
AC Q14439; B4DZ23; H9NIL9; J3KNC6; Q6NXF6;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=G-protein coupled receptor 176;
DE AltName: Full=HB-954;
GN Name=GPR176;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Fetal brain;
RX PubMed=7893747; DOI=10.1016/0167-4781(95)00002-x;
RA Hata S., Emi Y., Iyanagi T., Osumi T.;
RT "cDNA cloning of a putative G protein-coupled receptor from brain.";
RL Biochim. Biophys. Acta 1261:121-125(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RA Kaighin V.A., Martin A.L., Aronstam R.S.;
RT "Isolation of cDNA coding for 8 human genes.";
RL Submitted (DEC-2011) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16572171; DOI=10.1038/nature04601;
RA Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K.,
RA Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K.,
RA FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N.,
RA Abouelleil A., Arachchi H.M., Baradarani L., Birditt B., Bloom S.,
RA Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K.,
RA DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J.,
RA Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E.,
RA Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B.,
RA Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R.,
RA O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B.,
RA Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S.,
RA Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C.;
RT "Analysis of the DNA sequence and duplication history of human chromosome
RT 15.";
RL Nature 440:671-675(2006).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC TISSUE=Ovary;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Orphan receptor involved in normal circadian rhythm behavior.
CC Acts through the G-protein subclass G(z)-alpha and has an agonist-
CC independent basal activity to repress cAMP production.
CC {ECO:0000250|UniProtKB:Q80WT4}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q80WT4};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q14439-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q14439-2; Sequence=VSP_058845;
CC Name=3;
CC IsoId=Q14439-3; Sequence=VSP_058846;
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC {ECO:0000255|PROSITE-ProRule:PRU00521}.
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DR EMBL; D38449; BAA07481.1; -; mRNA.
DR EMBL; JQ308174; AFF59484.1; -; mRNA.
DR EMBL; AK302715; BAG63935.1; -; mRNA.
DR EMBL; AC012377; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC023908; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471125; EAW92386.1; -; Genomic_DNA.
DR EMBL; BC067106; AAH67106.1; -; mRNA.
DR CCDS; CCDS10051.1; -. [Q14439-1]
DR CCDS; CCDS61588.1; -. [Q14439-2]
DR CCDS; CCDS61589.1; -. [Q14439-3]
DR PIR; S53525; S53525.
DR RefSeq; NP_001258783.1; NM_001271854.1. [Q14439-3]
DR RefSeq; NP_001258784.1; NM_001271855.1. [Q14439-2]
DR RefSeq; NP_009154.1; NM_007223.2. [Q14439-1]
DR AlphaFoldDB; Q14439; -.
DR SMR; Q14439; -.
DR BioGRID; 116407; 1.
DR IntAct; Q14439; 3.
DR MINT; Q14439; -.
DR STRING; 9606.ENSP00000453076; -.
DR ChEMBL; CHEMBL4523869; -.
DR GlyGen; Q14439; 4 sites.
DR iPTMnet; Q14439; -.
DR PhosphoSitePlus; Q14439; -.
DR BioMuta; GPR176; -.
DR DMDM; 74754475; -.
DR EPD; Q14439; -.
DR jPOST; Q14439; -.
DR MassIVE; Q14439; -.
DR PaxDb; Q14439; -.
DR PeptideAtlas; Q14439; -.
DR PRIDE; Q14439; -.
DR ProteomicsDB; 5565; -.
DR ProteomicsDB; 59990; -.
DR Antibodypedia; 9997; 187 antibodies from 28 providers.
DR DNASU; 11245; -.
DR Ensembl; ENST00000299092.3; ENSP00000299092.3; ENSG00000166073.11. [Q14439-3]
DR Ensembl; ENST00000543580.7; ENSP00000439361.1; ENSG00000166073.11. [Q14439-2]
DR Ensembl; ENST00000561100.2; ENSP00000453076.1; ENSG00000166073.11. [Q14439-1]
DR GeneID; 11245; -.
DR KEGG; hsa:11245; -.
DR MANE-Select; ENST00000561100.2; ENSP00000453076.1; NM_007223.3; NP_009154.1.
DR UCSC; uc001zkj.3; human. [Q14439-1]
DR UCSC; uc010uck.3; human.
DR CTD; 11245; -.
DR DisGeNET; 11245; -.
DR GeneCards; GPR176; -.
DR HGNC; HGNC:32370; GPR176.
DR HPA; ENSG00000166073; Low tissue specificity.
DR MIM; 612183; gene.
DR neXtProt; NX_Q14439; -.
DR OpenTargets; ENSG00000166073; -.
DR PharmGKB; PA142671712; -.
DR VEuPathDB; HostDB:ENSG00000166073; -.
DR eggNOG; KOG3656; Eukaryota.
DR GeneTree; ENSGT00950000182998; -.
DR InParanoid; Q14439; -.
DR OMA; MVMIFIF; -.
DR OrthoDB; 602137at2759; -.
DR PhylomeDB; Q14439; -.
DR TreeFam; TF331506; -.
DR PathwayCommons; Q14439; -.
DR Reactome; R-HSA-418555; G alpha (s) signalling events.
DR Reactome; R-HSA-9660821; ADORA2B mediated anti-inflammatory cytokines production.
DR SignaLink; Q14439; -.
DR BioGRID-ORCS; 11245; 17 hits in 1066 CRISPR screens.
DR ChiTaRS; GPR176; human.
DR GeneWiki; GPR176; -.
DR GenomeRNAi; 11245; -.
DR Pharos; Q14439; Tdark.
DR PRO; PR:Q14439; -.
DR Proteomes; UP000005640; Chromosome 15.
DR RNAct; Q14439; protein.
DR Bgee; ENSG00000166073; Expressed in stromal cell of endometrium and 149 other tissues.
DR Genevisible; Q14439; HS.
DR GO; GO:0005887; C:integral component of plasma membrane; TAS:ProtInc.
DR GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR GO; GO:0045202; C:synapse; IEA:GOC.
DR GO; GO:0004930; F:G protein-coupled receptor activity; ISS:UniProtKB.
DR GO; GO:0007193; P:adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway; IEA:Ensembl.
DR GO; GO:0007268; P:chemical synaptic transmission; TAS:ProtInc.
DR GO; GO:0048512; P:circadian behavior; ISS:UniProtKB.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; ISS:UniProtKB.
DR CDD; cd15006; 7tmA_GPR176; 1.
DR InterPro; IPR043523; GPCR_176_Rhodpsn_7TM.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Biological rhythms; Cell membrane;
KW G-protein coupled receptor; Glycoprotein; Membrane; Receptor;
KW Reference proteome; Transducer; Transmembrane; Transmembrane helix.
FT CHAIN 1..515
FT /note="G-protein coupled receptor 176"
FT /id="PRO_0000069656"
FT TOPO_DOM 1..42
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 43..63
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 64..82
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 83..103
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 104..118
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 119..139
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 140..160
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 161..181
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 182..207
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 208..228
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 229..267
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 268..288
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 289..299
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 300..320
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 321..515
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..16
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 4
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 11
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 17
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 27
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..56
FT /note="MGHNGSWISPNASEPHNASGAEAAGVNRSALGEFGEAQLYRQFTTTVQVVIF
FT IGSL -> MPAPLLLISRT (in isoform 2)"
FT /evidence="ECO:0000303|Ref.2"
FT /id="VSP_058845"
FT VAR_SEQ 58..78
FT /note="GNFMVLWSTCRTTVFKSVTNR -> EFGNMEVTRKLDKSRLPGIK (in
FT isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_058846"
SQ SEQUENCE 515 AA; 56998 MW; DE21C57952BB6D12 CRC64;
MGHNGSWISP NASEPHNASG AEAAGVNRSA LGEFGEAQLY RQFTTTVQVV IFIGSLLGNF
MVLWSTCRTT VFKSVTNRFI KNLACSGICA SLVCVPFDII LSTSPHCCWW IYTMLFCKVV
KFLHKVFCSV TILSFPAIAL DRYYSVLYPL ERKISDAKSR ELVMYIWAHA VVASVPVFAV
TNVADIYATS TCTEVWSNSL GHLVYVLVYN ITTVIVPVVV VFLFLILIRR ALSASQKKKV
IIAALRTPQN TISIPYASQR EAELHATLLS MVMVFILCSV PYATLVVYQT VLNVPDTSVF
LLLTAVWLPK VSLLANPVLF LTVNKSVRKC LIGTLVQLHH RYSRRNVVST GSGMAEASLE
PSIRSGSQLL EMFHIGQQQI FKPTEDEEES EAKYIGSADF QAKEIFSTCL EGEQGPQFAP
SAPPLSTVDS VSQVAPAAPV EPETFPDKYS LQFGFGPFEL PPQWLSETRN SKKRLLPPLG
NTPEELIQTK VPKVGRVERK MSRNNKVSIF PKVDS
