ID   GP17_BPPH2              Reviewed;         166 AA.
AC   P03686; B3VMQ7;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 2.
DT   25-MAY-2022, entry version 69.
DE   RecName: Full=DNA replication protein 17;
DE   AltName: Full=Gene product 17;
DE            Short=gp17;
DE   AltName: Full=Protein p17;
GN   Name=17;
OS   Bacillus phage phi29 (Bacteriophage phi-29).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Salasmaviridae; Picovirinae; Salasvirus.
OX   NCBI_TaxID=10756;
OH   NCBI_TaxID=1423; Bacillus subtilis.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3007295; DOI=10.1016/0378-1119(85)90053-8;
RA   Garvey K.J., Yoshikawa H., Ito J.;
RT   "The complete sequence of the Bacillus phage phi 29 right early region.";
RL   Gene 40:301-309(1985).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Villegas A.P., Lingohr E.J., Ceyssens P.-J., Kropinski A.M.;
RL   Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-24.
RX   PubMed=6262789; DOI=10.1073/pnas.78.3.1336;
RA   Yoshikawa H., Friedmann T., Ito J.;
RT   "Nucleotide sequences at the termini of phi 29 DNA.";
RL   Proc. Natl. Acad. Sci. U.S.A. 78:1336-1340(1981).
RN   [4]
RP   INDUCTION, AND IDENTIFICATION.
RX   PubMed=6274853; DOI=10.1016/s0021-9258(19)68307-9;
RA   Murray C.L., Rabinowitz J.C.;
RT   "Nucleotide sequences of transcription and translation initiation regions
RT   in Bacillus phage phi 29 early genes.";
RL   J. Biol. Chem. 257:1053-1062(1982).
RN   [5]
RP   FUNCTION.
RX   PubMed=9858710; DOI=10.1016/s0378-1119(98)00167-x;
RA   Crucitti P., Lazaro J.M., Benes V., Salas M.;
RT   "Bacteriophage phi-29 early protein p17 is conditionally required for the
RT   first rounds of viral DNA replication.";
RL   Gene 223:135-142(1998).
RN   [6]
RP   SUBUNIT, AND INTERACTION WITH THE HISTONE-LIKE PROTEIN P6.
RX   PubMed=12480935; DOI=10.1074/jbc.m210289200;
RA   Crucitti P., Abril A.M., Salas M.;
RT   "Bacteriophage phi 29 early protein p17. Self-association and hetero-
RT   association with the viral histone-like protein p6.";
RL   J. Biol. Chem. 278:4906-4911(2003).
CC   -!- FUNCTION: Involved in the replication of viral DNA (PubMed:9858710). It
CC       is required at the very beginning of the virus amplification,
CC       conditions in which a low number of viral DNA molecules enter the host
CC       cell, possibly to recruit the limiting amount of initiation factors at
CC       the replication origins (PubMed:9858710). Once the infection process is
CC       established and the other replication proteins reach optimal
CC       concentration, it becomes dispensable (PubMed:9858710). Optimizes the
CC       binding of protein p6 at the viral DNA ends, thus favoring the
CC       initiation of replication (PubMed:12480935).
CC       {ECO:0000269|PubMed:12480935, ECO:0000269|PubMed:9858710}.
CC   -!- SUBUNIT: Homodimer. Interacts with the histone-like protein p6; this
CC       interaction optimizes the binding of protein p6 at the viral DNA ends,
CC       thus favoring the initiation of replication.
CC       {ECO:0000269|PubMed:12480935}.
CC   -!- INDUCTION: Expressed in the early phase of the viral replicative cycle
CC       (PubMed:6274853). The C2 promoter controls protein p17 transcription
CC       and is strongly inhibited by the viral histone-like protein p6,
CC       probably because of the formation of a p6-DNA nucleoprotein complex at
CC       the right end of the viral genome. Thus, protein p17 is synthesized
CC       very early after infection, and its synthesis declines later on due to
CC       the inhibition of the C2 promoter by protein p6.
CC       {ECO:0000269|PubMed:6274853}.
CC   -!- SIMILARITY: Belongs to the phi29likevirus protein p56 family.
CC       {ECO:0000305}.
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DR   EMBL; M14430; AAA88349.1; -; Genomic_DNA.
DR   EMBL; EU771092; ACE96044.1; -; Genomic_DNA.
DR   PIR; JN0030; JN0030.
DR   RefSeq; YP_002004550.1; NC_011048.1.
DR   TCDB; 9.B.80.1.1; the bacillus phage Phi29 (a podovirus) dna ejection system (Phi29-e) family.
DR   GeneID; 6446521; -.
DR   KEGG; vg:6446521; -.
DR   Proteomes; UP000001207; Genome.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   GO; GO:0039693; P:viral DNA genome replication; IDA:UniProtKB.
DR   InterPro; IPR035184; Phage_Gp17.
DR   Pfam; PF17549; Phage_Gp17; 1.
PE   1: Evidence at protein level;
KW   DNA replication; Early protein; Reference proteome; Viral DNA replication.
FT   CHAIN           1..166
FT                   /note="DNA replication protein 17"
FT                   /id="PRO_0000106604"
SQ   SEQUENCE   166 AA;  19170 MW;  204D7B480CC376CA CRC64;
     MNNYQLTINE VIDIINTNTE INKLVAKKEN LFPTDLYDLD KQELIAIILN SDFALSSIKR
     VLLEVTVEEL GTQDNDEDDE LEDLDGEIDR VDYIDKDGIR FDVPRETSPH VDKSIVTFND
     ELLDEANKIA KSIQEHDFND KAIEEAELKI FKNHLPSIYS MKKENK