ID   GP20_BPP22              Reviewed;         471 AA.
AC   Q01076; Q7PCH8;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 2.
DT   29-SEP-2021, entry version 74.
DE   RecName: Full=Internal virion protein gp20 {ECO:0000305};
DE   AltName: Full=Ejection protein gp20;
DE            Short=E protein gp20;
GN   Name=20;
OS   Salmonella phage P22 (Bacteriophage P22).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Podoviridae; Lederbergvirus.
OX   NCBI_TaxID=10754;
OH   NCBI_TaxID=90371; Salmonella typhimurium.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8464750; DOI=10.1093/nar/21.6.1499;
RA   Adhikari P., Berget P.B.;
RT   "Sequence of a DNA injection gene from Salmonella typhimurium phage P22.";
RL   Nucleic Acids Res. 21:1499-1499(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=11053393; DOI=10.1128/jb.182.22.6472-6481.2000;
RA   Vander Byl C.S., Kropinski A.M.B.;
RT   "Sequence of the genome of Salmonella bacteriophage P22.";
RL   J. Bacteriol. 182:6472-6481(2000).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=12562822; DOI=10.1128/jb.185.4.1475-1477.2003;
RA   Pedulla M.L., Ford M.E., Karthikeyan T., Houtz J.M., Hendrix R.W.,
RA   Hatfull G.F., Poteete A.R., Gilcrease E.B., Winn-Stapley D.A.,
RA   Casjens S.R.;
RT   "Corrected sequence of the bacteriophage P22 genome.";
RL   J. Bacteriol. 185:1475-1477(2003).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-92.
RX   PubMed=1522065; DOI=10.1128/jb.174.18.5869-5880.1992;
RA   Conlin C.A., Vimr E.R., Miller C.G.;
RT   "Oligopeptidase A is required for normal phage P22 development.";
RL   J. Bacteriol. 174:5869-5880(1992).
RN   [5]
RP   SUBCELLULAR LOCATION.
RX   PubMed=328927; DOI=10.1128/jvi.23.1.91-97.1977;
RA   Israel V.;
RT   "E proteins of bacteriophage P22. I. Identification and ejection from wild-
RT   type and defective particles.";
RL   J. Virol. 23:91-97(1977).
RN   [6]
RP   FUNCTION.
RX   PubMed=26245366; DOI=10.1016/j.virol.2015.07.006;
RA   Jin Y., Sdao S.M., Dover J.A., Porcek N.B., Knobler C.M., Gelbart W.M.,
RA   Parent K.N.;
RT   "Bacteriophage P22 ejects all of its internal proteins before its genome.";
RL   Virology 485:128-134(2015).
RN   [7]
RP   FUNCTION.
RX   PubMed=30886360; DOI=10.1038/s41564-019-0403-z;
RA   Wang C., Tu J., Liu J., Molineux I.J.;
RT   "Structural dynamics of bacteriophage P22 infection initiation revealed by
RT   cryo-electron tomography.";
RL   Nat. Microbiol. 4:1049-1056(2019).
RN   [8]
RP   STRUCTURE BY ELECTRON MICROSCOPY (12.5 ANGSTROMS) OF THE VIRION, AND
RP   FUNCTION.
RX   PubMed=29590587; DOI=10.1016/j.bpj.2018.01.026;
RA   McNulty R., Cardone G., Gilcrease E.B., Baker T.S., Casjens S.R.,
RA   Johnson J.E.;
RT   "Cryo-EM Elucidation of the Structure of Bacteriophage P22 Virions after
RT   Genome Release.";
RL   Biophys. J. 114:1295-1301(2018).
CC   -!- FUNCTION: Inner capsid protein that plays a role in viral DNA ejection
CC       into the host cell (PubMed:29590587). Ejected from the capsid after
CC       penetration the host outer membrane and has been released
CC       (PubMed:30886360). Assembles into a structure that extends the
CC       extracellular trans-envelope channel formed by the internal virion
CC       protein gp7 and probably gp16 (PubMed:30886360). This channel allows
CC       the delivery of the viral genome into the cell cytoplasm
CC       (PubMed:30886360). {ECO:0000269|PubMed:29590587,
CC       ECO:0000269|PubMed:30886360}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000269|PubMed:328927}.
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DR   EMBL; L07556; AAA62407.1; -; Genomic_DNA.
DR   EMBL; AF217253; AAF75054.1; -; Genomic_DNA.
DR   EMBL; M93985; AAA72116.1; -; Unassigned_DNA.
DR   EMBL; BK000583; DAA00994.1; -; Genomic_DNA.
DR   PIR; S35635; S35635.
DR   RefSeq; NP_059637.1; NC_002371.2.
DR   TCDB; 1.K.4.1.1; the phage p22 injectisome (p22 injectisome) family.
DR   GeneID; 1262812; -.
DR   KEGG; vg:1262812; -.
DR   Proteomes; UP000001795; Genome.
DR   Proteomes; UP000007960; Genome.
DR   GO; GO:0099002; P:viral genome ejection through host cell envelope, short tail mechanism; IDA:UniProtKB.
DR   InterPro; IPR031619; Inj_translocase.
DR   Pfam; PF16928; Inj_translocase; 1.
PE   1: Evidence at protein level;
KW   Late protein; Reference proteome;
KW   Viral genome ejection through host cell envelope;
KW   Viral penetration into host cytoplasm; Virion; Virus entry into host cell.
FT   CHAIN           1..471
FT                   /note="Internal virion protein gp20"
FT                   /id="PRO_0000077765"
FT   REGION          436..471
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   471 AA;  50101 MW;  EA6366D094D10A9D CRC64;
     MATWQQGINS GGFLAGIGAQ NENAPKARDI NATLGLIREN NDLARSGANN VALTGLRGLA
     GVADIYNQEQ QQKALNAFNQ VHANAWATGD PSGLFKFAQE NPAFVAQAQQ AFSGLNEQQR
     NDMGDLAMKA NVALSQGPEA YSKFITDNKD RLNRVGANPD WMIQTGVQNP EQLSHMLTTM
     SLGALGPEKA FAVQDKMVGR EIDRGRLAET IRSNKAGEGL QARGQNITMR GQDMSAATAR
     RGQDLATQRA NARTISGSEG NRVVQLADGR TVSVGGKLHG AGANAFYEGI DDNGNMVRVP
     ASAIAAPPTS AASAQNYAMK KDIDAIANAD ASALDFMTGM TGGAGNPAIG ADVRSRLTGK
     EQRQLYNSAQ RIQGRMQNQG VAAARDMGAS GINTIAEAKM YFQGMPQVDY SSPEAMQQSI
     REIQEYTNNY NQQYNVNVGN GGLKSPRQQP DTQQSAGGSY TSKSGIKFTV E