ID   GP5_EAVBU               Reviewed;         255 AA.
AC   P28995;
DT   01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-1992, sequence version 1.
DT   23-FEB-2022, entry version 80.
DE   RecName: Full=Glycoprotein 5;
DE            Short=Protein GP5;
DE   AltName: Full=G(L);
DE   Flags: Precursor;
GN   Name=GP5; ORFNames=5;
OS   Equine arteritis virus (strain Bucyrus) (EAV).
OC   Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC   Nidovirales; Arnidovirineae; Arteriviridae; Equarterivirinae;
OC   Alphaarterivirus; Alphaarterivirus equid.
OX   NCBI_TaxID=299386;
OH   NCBI_TaxID=9788; Equidae (horses).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=1851863; DOI=10.1128/jvi.65.6.2910-2920.1991;
RA   den Boon J.A., Snijder E.J., Chirnside E.D., de Vries A.A.F.,
RA   Horzinek M.C., Spaan W.J.M.;
RT   "Equine arteritis virus is not a togavirus but belongs to the
RT   coronaviruslike superfamily.";
RL   J. Virol. 65:2910-2920(1991).
RN   [2]
RP   SUBUNIT, DISULFIDE BONDS, AND MUTAGENESIS OF CYS-34; ASN-56; CYS-57;
RP   SER-58; CYS-63; CYS-66 AND CYS-80.
RX   PubMed=12477814; DOI=10.1128/jvi.77.1.97-104.2003;
RA   Snijder E.J., Dobbe J.C., Spaan W.J.;
RT   "Heterodimerization of the two major envelope proteins is essential for
RT   arterivirus infectivity.";
RL   J. Virol. 77:97-104(2003).
CC   -!- FUNCTION: Major envelope protein present in abundant amounts in the
CC       virion envelope. {ECO:0000250}.
CC   -!- SUBUNIT: Heterodimer with the membrane protein; disulfide-linked. This
CC       heterodimerization is required for transport to the Golgi complex.
CC       Interacts with glycoprotein 4 (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000305}; Multi-pass
CC       membrane protein {ECO:0000305}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative initiation; Named isoforms=2;
CC       Name=GP5; Synonyms=Glycoprotein 5;
CC         IsoId=P28995-1; Sequence=Displayed;
CC       Name=ORF5a; Synonyms=Protein ORF5a;
CC         IsoId=P0DJX9-1; Sequence=External;
CC   -!- SIMILARITY: Belongs to the arteriviridae GP5 protein family.
CC       {ECO:0000305}.
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DR   EMBL; X53459; CAA37544.1; -; Genomic_RNA.
DR   PIR; F39925; F39925.
DR   RefSeq; NP_065659.1; NC_002532.2.
DR   GeneID; 921342; -.
DR   KEGG; vg:921342; -.
DR   Proteomes; UP000000353; Genome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019031; C:viral envelope; IEA:UniProtKB-KW.
DR   GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR001332; Arteri_GP5.
DR   InterPro; IPR020403; EAV_Gp5.
DR   Pfam; PF00951; Arteri_Gl; 1.
DR   Pfam; PF15981; EAV_GP5; 1.
PE   1: Evidence at protein level;
KW   Alternative initiation; Disulfide bond; Glycoprotein; Membrane;
KW   Reference proteome; Signal; Transmembrane; Transmembrane helix;
KW   Viral envelope protein; Virion.
FT   SIGNAL          1..18
FT                   /evidence="ECO:0000255"
FT   CHAIN           19..255
FT                   /note="Glycoprotein 5"
FT                   /id="PRO_0000080882"
FT   TOPO_DOM        19..117
FT                   /note="Virion surface"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        118..138
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        151..171
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        56
FT                   /note="N-linked (GlcNAc...) asparagine; by host"
FT                   /evidence="ECO:0000255"
FT   DISULFID        34
FT                   /note="Interchain (with C-8 in membrane protein)"
FT                   /evidence="ECO:0000269|PubMed:12477814"
FT   MUTAGEN         34
FT                   /note="C->S: Complete loss of infectivity."
FT                   /evidence="ECO:0000269|PubMed:12477814"
FT   MUTAGEN         56
FT                   /note="N->G: Complete loss of infectivity."
FT                   /evidence="ECO:0000269|PubMed:12477814"
FT   MUTAGEN         56
FT                   /note="N->Q: Complete loss of infectivity."
FT                   /evidence="ECO:0000269|PubMed:12477814"
FT   MUTAGEN         56
FT                   /note="N->S: Complete loss of infectivity."
FT                   /evidence="ECO:0000269|PubMed:12477814"
FT   MUTAGEN         57
FT                   /note="C->S: Complete loss of infectivity."
FT                   /evidence="ECO:0000269|PubMed:12477814"
FT   MUTAGEN         58
FT                   /note="S->G: Complete loss of infectivity."
FT                   /evidence="ECO:0000269|PubMed:12477814"
FT   MUTAGEN         63
FT                   /note="C->S: Complete loss of infectivity."
FT                   /evidence="ECO:0000269|PubMed:12477814"
FT   MUTAGEN         66
FT                   /note="C->S: Complete loss of infectivity."
FT                   /evidence="ECO:0000269|PubMed:12477814"
FT   MUTAGEN         80
FT                   /note="C->S: Complete loss of infectivity."
FT                   /evidence="ECO:0000269|PubMed:12477814"
SQ   SEQUENCE   255 AA;  28673 MW;  4310EEED1134B399 CRC64;
     MLSMIVLLFL LWGAPSHAYF SYYTAQRFTD FTLCMLTDRG VIANLLRYDE HTALYNCSAS
     KTCWYCTFLD EQIITFGTDC DDTYAVPVAE VLEQAHGPYS ALFDDMPPFI YYGREFGIVV
     LDVFMFYPVL VLFFLSVLPY ATLILEMCVS ILFIIYGIYS GAYLAMGIFA ATLAIHSIVV
     LRQLLWLCLA WRYRCTLHAS FISAEGKVYP VDPGLPVAAV GNRLLVPGRP TIDYAVAYGS
     KVNLVRLGAA EVWEP