ID   GP7_BPP22               Reviewed;         229 AA.
AC   Q01074; Q7PCH9;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2004, sequence version 2.
DT   03-AUG-2022, entry version 79.
DE   RecName: Full=Internal virion protein gp7 {ECO:0000305};
DE   AltName: Full=Ejection protein gp7;
DE            Short=E protein gp7;
DE   Flags: Precursor;
GN   Name=7;
OS   Salmonella phage P22 (Bacteriophage P22).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Podoviridae; Lederbergvirus.
OX   NCBI_TaxID=10754;
OH   NCBI_TaxID=90371; Salmonella typhimurium.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1522065; DOI=10.1128/jb.174.18.5869-5880.1992;
RA   Conlin C.A., Vimr E.R., Miller C.G.;
RT   "Oligopeptidase A is required for normal phage P22 development.";
RL   J. Bacteriol. 174:5869-5880(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=11053393; DOI=10.1128/jb.182.22.6472-6481.2000;
RA   Vander Byl C.S., Kropinski A.M.B.;
RT   "Sequence of the genome of Salmonella bacteriophage P22.";
RL   J. Bacteriol. 182:6472-6481(2000).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=12562822; DOI=10.1128/jb.185.4.1475-1477.2003;
RA   Pedulla M.L., Ford M.E., Karthikeyan T., Houtz J.M., Hendrix R.W.,
RA   Hatfull G.F., Poteete A.R., Gilcrease E.B., Winn-Stapley D.A.,
RA   Casjens S.R.;
RT   "Corrected sequence of the bacteriophage P22 genome.";
RL   J. Bacteriol. 185:1475-1477(2003).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 225-229.
RX   PubMed=8464750; DOI=10.1093/nar/21.6.1499;
RA   Adhikari P., Berget P.B.;
RT   "Sequence of a DNA injection gene from Salmonella typhimurium phage P22.";
RL   Nucleic Acids Res. 21:1499-1499(1993).
RN   [5]
RP   PROTEIN SEQUENCE OF 21-31.
RX   PubMed=1853558; DOI=10.1016/0042-6822(91)90981-g;
RA   Eppler K., Wyckoff E., Goates J., Parr R., Casjens S.;
RT   "Nucleotide sequence of the bacteriophage P22 genes required for DNA
RT   packaging.";
RL   Virology 183:519-538(1991).
RN   [6]
RP   SUBCELLULAR LOCATION.
RX   PubMed=328927; DOI=10.1128/jvi.23.1.91-97.1977;
RA   Israel V.;
RT   "E proteins of bacteriophage P22. I. Identification and ejection from wild-
RT   type and defective particles.";
RL   J. Virol. 23:91-97(1977).
RN   [7]
RP   FUNCTION.
RX   PubMed=26245366; DOI=10.1016/j.virol.2015.07.006;
RA   Jin Y., Sdao S.M., Dover J.A., Porcek N.B., Knobler C.M., Gelbart W.M.,
RA   Parent K.N.;
RT   "Bacteriophage P22 ejects all of its internal proteins before its genome.";
RL   Virology 485:128-134(2015).
RN   [8]
RP   FUNCTION.
RX   PubMed=30886360; DOI=10.1038/s41564-019-0403-z;
RA   Wang C., Tu J., Liu J., Molineux I.J.;
RT   "Structural dynamics of bacteriophage P22 infection initiation revealed by
RT   cryo-electron tomography.";
RL   Nat. Microbiol. 4:1049-1056(2019).
RN   [9]
RP   STRUCTURE BY ELECTRON MICROSCOPY (12.5 ANGSTROMS) OF THE VIRION, AND
RP   FUNCTION.
RX   PubMed=29590587; DOI=10.1016/j.bpj.2018.01.026;
RA   McNulty R., Cardone G., Gilcrease E.B., Baker T.S., Casjens S.R.,
RA   Johnson J.E.;
RT   "Cryo-EM Elucidation of the Structure of Bacteriophage P22 Virions after
RT   Genome Release.";
RL   Biophys. J. 114:1295-1301(2018).
CC   -!- FUNCTION: Inner capsid protein that plays a role in viral DNA ejection
CC       into the host cell (PubMed:26245366, PubMed:29590587). Assembles into
CC       an extracellular trans-envelope channel completed by the internal
CC       virion proteins gp7 and probably gp16 (PubMed:30886360). This channel
CC       allows the delivery of the viral genome into the cell cytoplasm
CC       (PubMed:30886360). {ECO:0000269|PubMed:26245366,
CC       ECO:0000269|PubMed:29590587, ECO:0000269|PubMed:30886360}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000269|PubMed:328927}.
CC   -!- SIMILARITY: Belongs to the podoviruses gp7 family. {ECO:0000305}.
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DR   EMBL; M93985; AAA72115.1; -; Unassigned_DNA.
DR   EMBL; AF217253; AAF75053.1; -; Genomic_DNA.
DR   EMBL; BK000583; DAA00993.1; -; Genomic_DNA.
DR   EMBL; L07556; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   PIR; C43330; C43330.
DR   RefSeq; YP_063717.1; NC_002371.2.
DR   SMR; Q01074; -.
DR   TCDB; 1.K.4.1.1; the phage p22 injectisome (p22 injectisome) family.
DR   GeneID; 2944245; -.
DR   KEGG; vg:2944245; -.
DR   Proteomes; UP000001795; Genome.
DR   Proteomes; UP000007960; Genome.
DR   GO; GO:0099002; P:viral genome ejection through host cell envelope, short tail mechanism; IDA:UniProtKB.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Late protein; Reference proteome;
KW   Viral genome ejection through host cell envelope;
KW   Viral penetration into host cytoplasm; Virion; Virus entry into host cell.
FT   PROPEP          1..20
FT                   /note="Removed in mature form"
FT                   /evidence="ECO:0000269|PubMed:1853558"
FT                   /id="PRO_0000003374"
FT   CHAIN           21..229
FT                   /note="Internal virion protein gp7"
FT                   /id="PRO_0000003375"
FT   CONFLICT        3
FT                   /note="Y -> H (in Ref. 1 and 2)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   229 AA;  23433 MW;  92845934DA170627 CRC64;
     MLYAFTLGRK LRGEEPSYPE KGGKGGADKS AKYAAEAQKY AADLQNQQFN TIMNNLKPFT
     PLADKYIGSL EGLSSLEGQG QALNNYYNSQ QYQDLAGQAR YQNLAAAEAT GGLGSTATSN
     QLSAIAPTLG QQWLSGQMNN YQNLANIGLG ALQGQANAGQ TYANNMSQIS QQSAALAAAN
     ANRPSAMQSA IGGGASGAIA GAGLAKLIGS STPWGAAIGG GIGLLGSLF