GPA3_DICDI
ID GPA3_DICDI Reviewed; 570 AA.
AC Q54KV6; Q94470;
DT 04-DEC-2007, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Guanine nucleotide-binding protein alpha-3 subunit;
DE Short=G alpha-3;
GN Name=gpaC; Synonyms=Ga3; ORFNames=DDB_G0287031;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 67-570.
RX PubMed=9373143; DOI=10.1016/s0378-1119(97)00387-9;
RA Brandon M.A., Voglmaier S., Siddiqi A.A.;
RT "Molecular characterization of a Dictyostelium G-protein alpha-subunit
RT required for development.";
RL Gene 200:99-105(1997).
CC -!- FUNCTION: Guanine nucleotide-binding proteins (G proteins) are involved
CC as modulators or transducers in various transmembrane signaling
CC systems. G alpha-3 plays a role in development. G alpha-3 mutants fail
CC to aggregate.
CC -!- SUBUNIT: G proteins are composed of 3 units; alpha, beta and gamma. The
CC alpha chain contains the guanine nucleotide binding site.
CC -!- SIMILARITY: Belongs to the G-alpha family. {ECO:0000305}.
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DR EMBL; AAFI02000096; EAL63880.1; -; Genomic_DNA.
DR EMBL; U64319; AAB88395.1; -; Genomic_DNA.
DR RefSeq; XP_637414.1; XM_632322.1.
DR AlphaFoldDB; Q54KV6; -.
DR SMR; Q54KV6; -.
DR STRING; 44689.DDB0216258; -.
DR PaxDb; Q54KV6; -.
DR EnsemblProtists; EAL63880; EAL63880; DDB_G0287031.
DR GeneID; 8625946; -.
DR KEGG; ddi:DDB_G0287031; -.
DR dictyBase; DDB_G0287031; gpaC.
DR eggNOG; KOG0082; Eukaryota.
DR HOGENOM; CLU_014184_2_1_1; -.
DR InParanoid; Q54KV6; -.
DR OMA; WINFFDD; -.
DR PhylomeDB; Q54KV6; -.
DR PRO; PR:Q54KV6; -.
DR Proteomes; UP000002195; Chromosome 4.
DR GO; GO:0005834; C:heterotrimeric G-protein complex; IBA:GO_Central.
DR GO; GO:0001664; F:G protein-coupled receptor binding; IBA:GO_Central.
DR GO; GO:0031683; F:G-protein beta/gamma-subunit complex binding; IBA:GO_Central.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0007188; P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR CDD; cd00066; G-alpha; 1.
DR Gene3D; 1.10.400.10; -; 2.
DR Gene3D; 2.30.29.30; -; 1.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR001019; Gprotein_alpha_su.
DR InterPro; IPR011025; GproteinA_insert.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR001849; PH_domain.
DR PANTHER; PTHR10218; PTHR10218; 1.
DR Pfam; PF00503; G-alpha; 1.
DR Pfam; PF00169; PH; 1.
DR PRINTS; PR00318; GPROTEINA.
DR SMART; SM00275; G_alpha; 1.
DR SMART; SM00233; PH; 1.
DR SUPFAM; SSF47895; SSF47895; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS51882; G_ALPHA; 1.
DR PROSITE; PS50003; PH_DOMAIN; 1.
PE 3: Inferred from homology;
KW GTP-binding; Magnesium; Metal-binding; Nucleotide-binding;
KW Reference proteome; Transducer.
FT CHAIN 1..570
FT /note="Guanine nucleotide-binding protein alpha-3 subunit"
FT /id="PRO_0000312406"
FT DOMAIN 10..113
FT /note="PH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00145"
FT DOMAIN 144..570
FT /note="G-alpha"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01230"
FT REGION 147..160
FT /note="G1 motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01230"
FT REGION 254..321
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 386..394
FT /note="G2 motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01230"
FT REGION 410..419
FT /note="G3 motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01230"
FT REGION 479..486
FT /note="G4 motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01230"
FT REGION 542..547
FT /note="G5 motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01230"
FT COMPBIAS 254..276
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 283..321
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 152..159
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 159
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250"
FT BINDING 388..394
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 394
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250"
FT BINDING 414..418
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 483..486
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 544
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
SQ SEQUENCE 570 AA; 64973 MW; E28CCDA8D9C78C5B CRC64;
MDFNPVPPPR ETLRAYLSKK GHVVKNWKLR LFVLKPGSSY MEYFVDETKE QQHLPQGRVP
LYGTRVMEYV YNSNNNPREF CLSVETENKN WIISTGSKAQ QLEWIEAIKH AIESAVETDE
IKLQKHKSIE KQLQLDGKNK NKIPVLKLLL LGTGESGKST VVKQMKILHH KGFSKEEEEF
YRNLIYINLL DGMALLIHVI KENNIEIPQE TLSAIKNYSS WYRVYIEKRN KNGIRNNPIG
SMIPISILNN NKDNNNSNSS SLKNNSGGSS SSELDKKMTH LSLDGSTCIN SNSASPNGPS
SSTTTSTINT HNRSNSDGSS NNIFNVQDFG IPPIITNYIS TIWSDPVVQS EVMLQAQKYH
INESTQYYLN EIKRIGKPTY QPVNLDILKS RATTNGVVET DFNVNGEVIF RIVDVAGQRG
ERKKWINFFD DVTAIVFVAA INEYDQKLVE DNCTNRLHES LNLFDSICND STFPKTSIIL
FLNKIDLFRE KLKRTSINIC FPDYNGDQSY EKSSNYIKNN FLSKKKGGNG INSQNFIYFH
FTCATDTKSF ETVFNSVRDI IISKTLEFYC
