GPA3_ORYSJ
ID GPA3_ORYSJ Reviewed; 501 AA.
AC Q10AZ7; Q75LJ6;
DT 10-OCT-2018, integrated into UniProtKB/Swiss-Prot.
DT 22-AUG-2006, sequence version 1.
DT 03-AUG-2022, entry version 95.
DE RecName: Full=Protein GLUTELIN PRECURSOR ACCUMULATION 3 {ECO:0000303|PubMed:24488962};
GN Name=GPA3 {ECO:0000303|PubMed:24488962};
GN OrderedLocusNames=Os03g0835800 {ECO:0000312|EMBL:BAS87250.1},
GN LOC_Os03g61950 {ECO:0000312|EMBL:ABF99751.1};
GN ORFNames=OsJ_13268 {ECO:0000312|EMBL:EEE60253.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16109971; DOI=10.1101/gr.3869505;
RG The rice chromosome 3 sequencing consortium;
RA Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA Jin W., Lee H.R., Jiang J., Jackson S.;
RT "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT and diverged grass species.";
RL Genome Res. 15:1284-1291(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [7]
RP FUNCTION, INTERACTION WITH VPS9A, SUBCELLULAR LOCATION, TISSUE SPECIFICITY,
RP DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=24488962; DOI=10.1105/tpc.113.121376;
RA Ren Y., Wang Y., Liu F., Zhou K., Ding Y., Zhou F., Wang Y., Liu K.,
RA Gan L., Ma W., Han X., Zhang X., Guo X., Wu F., Cheng Z., Wang J., Lei C.,
RA Lin Q., Jiang L., Wu C., Bao Y., Wang H., Wan J.;
RT "GLUTELIN PRECURSOR ACCUMULATION3 encodes a regulator of post-Golgi
RT vesicular traffic essential for vacuolar protein sorting in rice
RT endosperm.";
RL Plant Cell 26:410-425(2014).
CC -!- FUNCTION: Acts synergistically with RAB5A and VPS9A to regulate dense
CC vesicle-mediated post-Golgi trafficking of major storage proteins in
CC the seed endosperm during grain filling and maturation.
CC {ECO:0000269|PubMed:24488962}.
CC -!- SUBUNIT: Interacts (via C-terminus) with VPS9A.
CC {ECO:0000269|PubMed:24488962}.
CC -!- SUBCELLULAR LOCATION: Prevacuolar compartment
CC {ECO:0000269|PubMed:24488962}. Golgi apparatus, trans-Golgi network
CC {ECO:0000269|PubMed:24488962}. Protein storage vacuole
CC {ECO:0000269|PubMed:24488962}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q10AZ7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q10AZ7-2; Sequence=VSP_059820, VSP_059821;
CC -!- TISSUE SPECIFICITY: Highly expressed in leaves.
CC {ECO:0000269|PubMed:24488962}.
CC -!- DEVELOPMENTAL STAGE: During endosperm development, the expression of
CC GPA3 is low at an early stage, peaks at 18 days after flowering (DAF),
CC and slowly decreases after 21 DAF. {ECO:0000269|PubMed:24488962}.
CC -!- DISRUPTION PHENOTYPE: Accumulation of proglutelins in seed endosperm
CC (PubMed:24488962). Mistargeting of dense vesicles (DVs) to the type II
CC protein bodies (PBII) protein storage vacuoles and reduction of PBII
CC size in endosperm. Formation of paramural bodies (PMBs) secretory
CC vesicle-like structures charged with DVs in endosperm
CC (PubMed:24488962). {ECO:0000269|PubMed:24488962}.
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DR EMBL; AC092557; AAR88573.1; -; Genomic_DNA.
DR EMBL; DP000009; ABF99751.1; -; Genomic_DNA.
DR EMBL; DP000009; ABF99752.1; -; Genomic_DNA.
DR EMBL; AP008209; BAF13734.1; -; Genomic_DNA.
DR EMBL; AP014959; BAS87250.1; -; Genomic_DNA.
DR EMBL; AP014959; BAS87251.1; -; Genomic_DNA.
DR EMBL; CM000140; EEE60253.1; -; Genomic_DNA.
DR EMBL; AK102903; BAG95773.1; -; mRNA.
DR RefSeq; XP_015629757.1; XM_015774271.1. [Q10AZ7-1]
DR RefSeq; XP_015629759.1; XM_015774273.1. [Q10AZ7-1]
DR AlphaFoldDB; Q10AZ7; -.
DR SMR; Q10AZ7; -.
DR STRING; 4530.OS03T0835800-01; -.
DR PaxDb; Q10AZ7; -.
DR PRIDE; Q10AZ7; -.
DR EnsemblPlants; Os03t0835800-01; Os03t0835800-01; Os03g0835800. [Q10AZ7-2]
DR EnsemblPlants; Os03t0835800-02; Os03t0835800-02; Os03g0835800. [Q10AZ7-1]
DR GeneID; 4334700; -.
DR Gramene; Os03t0835800-01; Os03t0835800-01; Os03g0835800. [Q10AZ7-2]
DR Gramene; Os03t0835800-02; Os03t0835800-02; Os03g0835800. [Q10AZ7-1]
DR KEGG; osa:4334700; -.
DR eggNOG; KOG0379; Eukaryota.
DR HOGENOM; CLU_027031_0_0_1; -.
DR OMA; KAIHENQ; -.
DR OrthoDB; 1263405at2759; -.
DR PlantReactome; R-OSA-9626305; Regulatory network of nutrient accumulation.
DR Proteomes; UP000000763; Chromosome 3.
DR Proteomes; UP000007752; Chromosome 3.
DR Proteomes; UP000059680; Chromosome 3.
DR GO; GO:0000325; C:plant-type vacuole; IDA:UniProtKB.
DR GO; GO:0000326; C:protein storage vacuole; IDA:UniProtKB.
DR GO; GO:0005802; C:trans-Golgi network; IDA:UniProtKB.
DR GO; GO:0006886; P:intracellular protein transport; IMP:UniProtKB.
DR Gene3D; 2.120.10.80; -; 2.
DR InterPro; IPR015915; Kelch-typ_b-propeller.
DR InterPro; IPR006652; Kelch_1.
DR SMART; SM00612; Kelch; 3.
DR SUPFAM; SSF117281; SSF117281; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Golgi apparatus; Kelch repeat; Protein transport;
KW Reference proteome; Repeat; Transport; Vacuole.
FT CHAIN 1..501
FT /note="Protein GLUTELIN PRECURSOR ACCUMULATION 3"
FT /id="PRO_0000445093"
FT REPEAT 40..86
FT /note="Kelch 1"
FT /evidence="ECO:0000255"
FT REPEAT 96..145
FT /note="Kelch 2"
FT /evidence="ECO:0000255"
FT REPEAT 147..196
FT /note="Kelch 3"
FT /evidence="ECO:0000255"
FT REPEAT 198..247
FT /note="Kelch 4"
FT /evidence="ECO:0000255"
FT REPEAT 254..310
FT /note="Kelch 5"
FT /evidence="ECO:0000255"
FT REPEAT 312..357
FT /note="Kelch 6"
FT /evidence="ECO:0000255"
FT REGION 341..371
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 347..371
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 460..476
FT /note="ENSFTSFYEKEVHRFFH -> YGSHSCVFPLLSLFTLS (in isoform
FT 2)"
FT /id="VSP_059820"
FT VAR_SEQ 477..501
FT /note="Missing (in isoform 2)"
FT /id="VSP_059821"
SQ SEQUENCE 501 AA; 55248 MW; 256DF78FFFE49DCF CRC64;
MTPQLQPKQM HWARADSSDF GGQIPAPRSG HTAVSIGKSK VVVFGGFADK RFLSDIAVYD
VENRIWYTPE CNGSGSDGQA GPSPRAFHVA IVIDCNMFIF GGRSGGKRLG DFWMLDTDIW
QWSELTGFGD LPSPREFAAA SAIGNRKIVM YGGWDGKKWL SDVYIMDTMS LEWTELSVTG
SVPPPRCGHS ATMIEKRLLV FGGRGGAGPI MGDLWALKGV TEEDNETPGW TQLKLPGQSP
SPRCGHSVTS GGPYLLLFGG HGTGGWLSRY DVYYNECIIL DRVSVQWKLL ATSNEPPPPR
AYHSMTCIGS RFLLFGGFDG KNTFGDLWWL VPEGDPIAKR DLVPNVDSDS KPSNVTGGAQ
HSASQESQAG ESPMIDLAKR LGISLSLEAS ASFVDEINDK ELIELSSMLF GESPPTGDQH
ACIQALRDHW TSIPANSIQL QELGPLLRDY QRLILRRYLE NSFTSFYEKE VHRFFHLKNA
SELRMDDIPI LLMEYGKLLS T
