GPA4_CAEEL
ID GPA4_CAEEL Reviewed; 359 AA.
AC Q9BIG5; O44409;
DT 27-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=Guanine nucleotide-binding protein alpha-4 subunit;
GN Name=gpa-4; ORFNames=T07A9.7;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Bristol N2;
RA Cuppen E., Jansen G., Plasterk R.H.A.;
RT "Interaction analysis of the complete G-alpha subfamily of heterotrimeric G
RT proteins from Caenorhabditis elegans.";
RL Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=10192394; DOI=10.1038/7753;
RA Jansen G., Thijssen K.L., Werner P., van der Horst M., Hazendonk E.,
RA Plasterk R.H.A.;
RT "The complete family of genes encoding G proteins of Caenorhabditis
RT elegans.";
RL Nat. Genet. 21:414-419(1999).
CC -!- FUNCTION: Guanine nucleotide-binding proteins (G proteins) are involved
CC as modulators or transducers in various transmembrane signaling
CC systems.
CC -!- SUBUNIT: G proteins are composed of 3 units; alpha, beta and gamma. The
CC alpha chain contains the guanine nucleotide binding site.
CC -!- SIMILARITY: Belongs to the G-alpha family. G(i/o/t/z) subfamily.
CC {ECO:0000305}.
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DR EMBL; AY008127; AAG32080.1; -; mRNA.
DR EMBL; FO081716; CCD73978.1; -; Genomic_DNA.
DR PIR; T32578; T32578.
DR RefSeq; NP_499921.2; NM_067520.6.
DR AlphaFoldDB; Q9BIG5; -.
DR SMR; Q9BIG5; -.
DR STRING; 6239.T07A9.7; -.
DR PaxDb; Q9BIG5; -.
DR PRIDE; Q9BIG5; -.
DR EnsemblMetazoa; T07A9.7.1; T07A9.7.1; WBGene00001666.
DR GeneID; 176865; -.
DR KEGG; cel:CELE_T07A9.7; -.
DR UCSC; T07A9.7; c. elegans.
DR CTD; 176865; -.
DR WormBase; T07A9.7; CE31599; WBGene00001666; gpa-4.
DR eggNOG; KOG0082; Eukaryota.
DR GeneTree; ENSGT00940000165593; -.
DR HOGENOM; CLU_014184_6_0_1; -.
DR InParanoid; Q9BIG5; -.
DR OMA; XYLNDER; -.
DR OrthoDB; 754573at2759; -.
DR PhylomeDB; Q9BIG5; -.
DR Reactome; R-CEL-170670; Adenylate cyclase inhibitory pathway.
DR Reactome; R-CEL-2485179; Activation of the phototransduction cascade.
DR Reactome; R-CEL-2514859; Inactivation, recovery and regulation of the phototransduction cascade.
DR Reactome; R-CEL-392170; ADP signalling through P2Y purinoceptor 12.
DR Reactome; R-CEL-4086398; Ca2+ pathway.
DR Reactome; R-CEL-418594; G alpha (i) signalling events.
DR Reactome; R-CEL-9717207; Sensory perception of sweet, bitter, and umami (glutamate) taste.
DR PRO; PR:Q9BIG5; -.
DR Proteomes; UP000001940; Chromosome IV.
DR GO; GO:0005929; C:cilium; IDA:UniProtKB.
DR GO; GO:0005834; C:heterotrimeric G-protein complex; IBA:GO_Central.
DR GO; GO:0097730; C:non-motile cilium; IDA:UniProtKB.
DR GO; GO:0001664; F:G protein-coupled receptor binding; IBA:GO_Central.
DR GO; GO:0031683; F:G-protein beta/gamma-subunit complex binding; IBA:GO_Central.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0007188; P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR CDD; cd00066; G-alpha; 1.
DR Gene3D; 1.10.400.10; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR001408; Gprotein_alpha_I.
DR InterPro; IPR001019; Gprotein_alpha_su.
DR InterPro; IPR011025; GproteinA_insert.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR10218; PTHR10218; 1.
DR Pfam; PF00503; G-alpha; 1.
DR PRINTS; PR00318; GPROTEINA.
DR PRINTS; PR00441; GPROTEINAI.
DR SMART; SM00275; G_alpha; 1.
DR SUPFAM; SSF47895; SSF47895; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS51882; G_ALPHA; 1.
PE 2: Evidence at transcript level;
KW GTP-binding; Lipoprotein; Magnesium; Metal-binding; Myristate;
KW Nucleotide-binding; Palmitate; Reference proteome; Transducer.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000255"
FT CHAIN 2..359
FT /note="Guanine nucleotide-binding protein alpha-4 subunit"
FT /id="PRO_0000203635"
FT DOMAIN 31..359
FT /note="G-alpha"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01230"
FT REGION 34..47
FT /note="G1 motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01230"
FT REGION 176..184
FT /note="G2 motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01230"
FT REGION 199..208
FT /note="G3 motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01230"
FT REGION 268..275
FT /note="G4 motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01230"
FT REGION 329..334
FT /note="G5 motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01230"
FT BINDING 39..46
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 46
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250"
FT BINDING 178..184
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 203..207
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 272..275
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 331
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT LIPID 2
FT /note="N-myristoyl glycine"
FT /evidence="ECO:0000255"
FT LIPID 3
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 359 AA; 41671 MW; 04CD363CE9E90D2B CRC64;
MGCFHSTGSE AKKRSKLIDE QLRHDHERCV GEIKLLLLGA GESGKSTIVR QMRILHETGF
NKQEQMAYRP VVFSNMVQSM LAILKAMQPL NISFTDAARE EDARMFISHF LHVNNAELSE
AFSLELSDLM KQLWMDEGVK KCVKRAHEYQ LNDSAEYYFN ALDRISSSSY LPTQDDILRA
RVKSTGIVET TFMYKDLCFK MFDVGGQRSE RKKWIHCFDS VTAVIFCVAL SEYDLRLAED
QTMNRMHESM QLFDSIVNNC WFTETSIILF LNKMDIFEER IRYTPLTVCF PEYQGGMTIT
ETSTFIQSRF EILNKRQTPA QKEIYSHFTC ATDTNNIRFV FDAVTDIIIR NNLYLCGLY
