GPA4_DICDI
ID GPA4_DICDI Reviewed; 345 AA.
AC P34042; Q54MU7;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1994, sequence version 1.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Guanine nucleotide-binding protein alpha-4 subunit;
DE Short=G alpha-4;
GN Name=gpaD; ORFNames=DDB_G0285425;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1910174; DOI=10.1073/pnas.88.18.8213;
RA Hadwiger J.A., Wilkie T.M., Strathmann M., Firtel R.A.;
RT "Identification of Dictyostelium G alpha genes expressed during
RT multicellular development.";
RL Proc. Natl. Acad. Sci. U.S.A. 88:8213-8217(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=AX2;
RX PubMed=16926386; DOI=10.1074/mcp.m600113-mcp200;
RA Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M.,
RA Soldati T.;
RT "Proteomics fingerprinting of phagosome maturation and evidence for the
RT role of a Galpha during uptake.";
RL Mol. Cell. Proteomics 5:2228-2243(2006).
CC -!- FUNCTION: Guanine nucleotide-binding proteins (G proteins) are involved
CC as modulators or transducers in various transmembrane signaling
CC systems. G alpha-4 plays a role in morphogenesis of the multicellular
CC structure.
CC -!- SUBUNIT: G proteins are composed of 3 units; alpha, beta and gamma. The
CC alpha chain contains the guanine nucleotide binding site.
CC -!- INTERACTION:
CC P34042; Q54QB1: erkB; NbExp=2; IntAct=EBI-2905587, EBI-2905550;
CC -!- SIMILARITY: Belongs to the G-alpha family. {ECO:0000305}.
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DR EMBL; S55498; AAB19793.1; -; Genomic_DNA.
DR EMBL; AAFI02000079; EAL64550.1; -; Genomic_DNA.
DR PIR; A40990; A40990.
DR RefSeq; XP_638196.1; XM_633104.1.
DR AlphaFoldDB; P34042; -.
DR SMR; P34042; -.
DR IntAct; P34042; 1.
DR STRING; 44689.DDB0216411; -.
DR PaxDb; P34042; -.
DR EnsemblProtists; EAL64550; EAL64550; DDB_G0285425.
DR GeneID; 8625242; -.
DR KEGG; ddi:DDB_G0285425; -.
DR dictyBase; DDB_G0285425; gpaD.
DR eggNOG; KOG0082; Eukaryota.
DR HOGENOM; CLU_014184_6_0_1; -.
DR InParanoid; P34042; -.
DR OMA; AVTYLWN; -.
DR PhylomeDB; P34042; -.
DR Reactome; R-DDI-112043; PLC beta mediated events.
DR Reactome; R-DDI-170660; Adenylate cyclase activating pathway.
DR Reactome; R-DDI-170670; Adenylate cyclase inhibitory pathway.
DR Reactome; R-DDI-202040; G-protein activation.
DR Reactome; R-DDI-2485179; Activation of the phototransduction cascade.
DR Reactome; R-DDI-2514859; Inactivation, recovery and regulation of the phototransduction cascade.
DR Reactome; R-DDI-399997; Acetylcholine regulates insulin secretion.
DR Reactome; R-DDI-4086398; Ca2+ pathway.
DR Reactome; R-DDI-416476; G alpha (q) signalling events.
DR Reactome; R-DDI-416482; G alpha (12/13) signalling events.
DR Reactome; R-DDI-418594; G alpha (i) signalling events.
DR Reactome; R-DDI-418597; G alpha (z) signalling events.
DR Reactome; R-DDI-434316; Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion.
DR Reactome; R-DDI-9013148; CDC42 GTPase cycle.
DR Reactome; R-DDI-9013149; RAC1 GTPase cycle.
DR PRO; PR:P34042; -.
DR Proteomes; UP000002195; Chromosome 4.
DR GO; GO:0005834; C:heterotrimeric G-protein complex; IBA:GO_Central.
DR GO; GO:0045335; C:phagocytic vesicle; HDA:dictyBase.
DR GO; GO:0005886; C:plasma membrane; TAS:dictyBase.
DR GO; GO:0001664; F:G protein-coupled receptor binding; IBA:GO_Central.
DR GO; GO:0031683; F:G-protein beta/gamma-subunit complex binding; IBA:GO_Central.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0019887; F:protein kinase regulator activity; IMP:dictyBase.
DR GO; GO:0007188; P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0031152; P:aggregation involved in sorocarp development; TAS:dictyBase.
DR GO; GO:0006935; P:chemotaxis; TAS:dictyBase.
DR GO; GO:0043326; P:chemotaxis to folate; IMP:dictyBase.
DR GO; GO:0000165; P:MAPK cascade; IMP:dictyBase.
DR GO; GO:0043406; P:positive regulation of MAP kinase activity; IMP:dictyBase.
DR GO; GO:1905301; P:regulation of macropinocytosis; IMP:dictyBase.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; NAS:dictyBase.
DR CDD; cd00066; G-alpha; 1.
DR Gene3D; 1.10.400.10; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR001019; Gprotein_alpha_su.
DR InterPro; IPR011025; GproteinA_insert.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR10218; PTHR10218; 1.
DR Pfam; PF00503; G-alpha; 1.
DR PRINTS; PR00318; GPROTEINA.
DR SMART; SM00275; G_alpha; 1.
DR SUPFAM; SSF47895; SSF47895; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS51882; G_ALPHA; 1.
PE 1: Evidence at protein level;
KW GTP-binding; Magnesium; Metal-binding; Nucleotide-binding;
KW Reference proteome; Transducer.
FT CHAIN 1..345
FT /note="Guanine nucleotide-binding protein alpha-4 subunit"
FT /id="PRO_0000203662"
FT DOMAIN 30..345
FT /note="G-alpha"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01230"
FT REGION 33..46
FT /note="G1 motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01230"
FT REGION 169..177
FT /note="G2 motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01230"
FT REGION 192..201
FT /note="G3 motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01230"
FT REGION 261..268
FT /note="G4 motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01230"
FT REGION 318..323
FT /note="G5 motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01230"
FT BINDING 38..45
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 45
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250"
FT BINDING 171..177
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 177
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250"
FT BINDING 196..200
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 265..268
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 320
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
SQ SEQUENCE 345 AA; 40138 MW; 6B92FF0FFF5775B9 CRC64;
MRFKCFGSEE TEQSSKIDKS IETDRRKLRK DVKLLLLGPG ESGKSTIFKQ MKIIQEDGGY
SVEELLEYRA FVYSNCISQM EALLTASAKL NIELEVENKQ RAANVLRRTI GNEPWLLLAA
DIKHLWEDKG IKETYAQKDK HFQLNDSAAY FFDNIDRYMR EDFVPNEQDV LRCRVRTTGI
QESEFTFDKI RLKIVDVGGQ RSQRRKWIHC FDCVTAVIFV AAMSDYDQVL REDESVNRTR
ESLALFKEIV NCDYFKETPI VLFLNKKDLF KEKLKRVPLQ SCFSDYTGPN KYKDAAMFIQ
SQYLAQGPSP RTIYTHATCA VDTENIKFVF RAVRQTILSQ ALEHF
