GPA6_DICDI
ID GPA6_DICDI Reviewed; 347 AA.
AC P34044; Q54RH3;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 04-DEC-2007, sequence version 2.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Guanine nucleotide-binding protein alpha-6 subunit;
DE Short=G alpha-6;
GN Name=gpaF; ORFNames=DDB_G0283151;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 44-196.
RC STRAIN=AX3;
RX PubMed=1930158; DOI=10.1016/0006-291x(91)91690-e;
RA Wu L., Devreotes P.N.;
RT "Dictyostelium transiently expresses eight distinct G-protein alpha-
RT subunits during its developmental program.";
RL Biochem. Biophys. Res. Commun. 179:1141-1147(1991).
CC -!- FUNCTION: Guanine nucleotide-binding proteins (G proteins) are involved
CC as modulators or transducers in various transmembrane signaling
CC systems. G alpha-6 is involved in the folic acid chemotaxis signal
CC transduction pathway.
CC -!- SUBUNIT: G proteins are composed of 3 units; alpha, beta and gamma. The
CC alpha chain contains the guanine nucleotide binding site.
CC -!- DEVELOPMENTAL STAGE: Expressed primarily in vegetative cells.
CC -!- SIMILARITY: Belongs to the G-alpha family. {ECO:0000305}.
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DR EMBL; AAFI02000051; EAL65814.1; -; Genomic_DNA.
DR PIR; JH0247; JH0247.
DR RefSeq; XP_639172.1; XM_634080.1.
DR AlphaFoldDB; P34044; -.
DR SMR; P34044; -.
DR STRING; 44689.DDB0230127; -.
DR PaxDb; P34044; -.
DR EnsemblProtists; EAL65814; EAL65814; DDB_G0283151.
DR GeneID; 8623948; -.
DR KEGG; ddi:DDB_G0283151; -.
DR dictyBase; DDB_G0283151; gpaF.
DR eggNOG; KOG0082; Eukaryota.
DR HOGENOM; CLU_014184_6_0_1; -.
DR InParanoid; P34044; -.
DR OMA; ICKPDYM; -.
DR PhylomeDB; P34044; -.
DR Reactome; R-DDI-112043; PLC beta mediated events.
DR Reactome; R-DDI-170660; Adenylate cyclase activating pathway.
DR Reactome; R-DDI-170670; Adenylate cyclase inhibitory pathway.
DR Reactome; R-DDI-202040; G-protein activation.
DR Reactome; R-DDI-2485179; Activation of the phototransduction cascade.
DR Reactome; R-DDI-2514859; Inactivation, recovery and regulation of the phototransduction cascade.
DR Reactome; R-DDI-399997; Acetylcholine regulates insulin secretion.
DR Reactome; R-DDI-4086398; Ca2+ pathway.
DR Reactome; R-DDI-416476; G alpha (q) signalling events.
DR Reactome; R-DDI-416482; G alpha (12/13) signalling events.
DR Reactome; R-DDI-418594; G alpha (i) signalling events.
DR Reactome; R-DDI-418597; G alpha (z) signalling events.
DR Reactome; R-DDI-434316; Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion.
DR Reactome; R-DDI-9013148; CDC42 GTPase cycle.
DR Reactome; R-DDI-9013149; RAC1 GTPase cycle.
DR PRO; PR:P34044; -.
DR Proteomes; UP000002195; Chromosome 4.
DR GO; GO:0005834; C:heterotrimeric G-protein complex; IBA:GO_Central.
DR GO; GO:0001664; F:G protein-coupled receptor binding; IBA:GO_Central.
DR GO; GO:0031683; F:G-protein beta/gamma-subunit complex binding; IBA:GO_Central.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0007188; P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR CDD; cd00066; G-alpha; 1.
DR Gene3D; 1.10.400.10; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR001019; Gprotein_alpha_su.
DR InterPro; IPR011025; GproteinA_insert.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR10218; PTHR10218; 1.
DR Pfam; PF00503; G-alpha; 1.
DR PRINTS; PR00318; GPROTEINA.
DR SMART; SM00275; G_alpha; 1.
DR SUPFAM; SSF47895; SSF47895; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS51882; G_ALPHA; 1.
PE 2: Evidence at transcript level;
KW GTP-binding; Magnesium; Metal-binding; Nucleotide-binding;
KW Reference proteome; Transducer.
FT CHAIN 1..347
FT /note="Guanine nucleotide-binding protein alpha-6 subunit"
FT /id="PRO_0000203664"
FT DOMAIN 30..347
FT /note="G-alpha"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01230"
FT REGION 33..46
FT /note="G1 motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01230"
FT REGION 170..178
FT /note="G2 motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01230"
FT REGION 193..202
FT /note="G3 motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01230"
FT REGION 262..269
FT /note="G4 motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01230"
FT REGION 320..325
FT /note="G5 motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01230"
FT BINDING 38..45
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 45
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250"
FT BINDING 172..178
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 178
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250"
FT BINDING 197..201
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 266..269
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 322
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
SQ SEQUENCE 347 AA; 40085 MW; CB2F8AC934A0EAEC CRC64;
MAFLCKSNDN TKVSMEIEKS IKDDKRTFLG ITQVLLLGAG ESGKSTIFKQ LKILQENGLW
TTKELQEFSN TIYINIITQL QVLINAAESI GIQLQPENIQ LSKDYLELNH SSVTWTKEIG
ENSVRLWNDS GIKSTFEKRD KEFQLNDSAE YFFNNLQRVT QDNYIPTPQD ALRARVTTKG
IIEADVTFDN INMKIIDVGG QRSQRRKWIH CFDKVSAVIY VAGLSEYDQA LREDINVNKM
DESLSLFKEI CNSKWFDSSS IILFLNKKDM FIEKLKRVPF KTYDKKYTGE NSFEAVSLHI
QRKFESVKID PNKKIYTHFT IAVDTENIEF VFKVIKKIIV GHVMDLV
